Restriction map for pF1KB3524
 (Linear) (Six-base) MAPSORT of: KIBB3524.seq  Check: 9139  from: 1  to: 1380



KIBB3524.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AatII G_ACGT'C

Cuts at:      0     456    1380 
   Size:        456     924
 
AccI GT'mk_AC

Cuts at:      0     309    1380 
   Size:        309    1071
 
AclI AA'CG_TT

Cuts at:      0     749    1380 
   Size:        749     631
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     817     943    1106    1380 
   Size:        817     126     163     274

  Fragments arranged by size: 

                 817     274     163     126
 
AlwNI CAG_nnn'CTG

Cuts at:      0     514     835    1380 
   Size:        514     321     545

  Fragments arranged by size: 

                 545     514     321
 
ApoI r'AATT_y

Cuts at:      0     382     394     716     862    1380 
   Size:        382      12     322     146     518

  Fragments arranged by size: 

                 518     382     322     146      12
 
AvaI C'yCGr_G

Cuts at:      0      79    1380 
   Size:         79    1301
 
BamHI G'GATC_C

Cuts at:      0     705    1380 
   Size:        705     675
 
BanII G_rGCy'C

Cuts at:      0      80     845    1380 
   Size:         80     765     535

  Fragments arranged by size: 

                 765     535      80
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1373    1380 
   Size:       1373       7
 
BmtI G_CTAG'C

Cuts at:      0     937    1380 
   Size:        937     443
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     701    1380 
   Size:        112     589     679

  Fragments arranged by size: 

                 679     589     112
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1372    1380 
   Size:       1372       8
 
BsaAI yAC'GTr

Cuts at:      0    1189    1380 
   Size:       1189     191
 
BsaBI GATnn'nnATC

Cuts at:      0     950    1380 
   Size:        950     430
 
BsaHI Gr'CG_yC

Cuts at:      0     453    1380 
   Size:        453     927
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     747     777    1380 
   Size:        747      30     603

  Fragments arranged by size: 

                 747     603      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     580     655     658     661     664     667     670     673 
   Size:        580      75       3       3       3       3       3       3

Cuts at:    673     715    1115    1380 
   Size:         42     400     265

  Fragments arranged by size: 

                 580     400     265      75      42       3       3       3
                   3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     115     157     199    1380 
   Size:        115      42      42    1181

  Fragments arranged by size: 

                1181     115      42      42
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     119    1380 
   Size:        119    1261
 
Bsp1286I G_dGCh'C

Cuts at:      0      80     845    1380 
   Size:         80     765     535

  Fragments arranged by size: 

                 765     535      80
 
BspHI T'CATG_A

Cuts at:      0     399     543    1380 
   Size:        399     144     837

  Fragments arranged by size: 

                 837     399     144
 
BsrBI CCG'CTC

Cuts at:      0     364    1380 
   Size:        364    1016
 
BstYI r'GATC_y

Cuts at:      0     705     831    1058    1380 
   Size:        705     126     227     322

  Fragments arranged by size: 

                 705     322     227     126
 
Bsu36I CC'TnA_GG

Cuts at:      0     388     710    1380 
   Size:        388     322     670

  Fragments arranged by size: 

                 670     388     322
 
DraI TTT'AAA

Cuts at:      0     743    1380 
   Size:        743     637
 
EaeI y'GGCC_r

Cuts at:      0       2     365    1380 
   Size:          2     363    1015

  Fragments arranged by size: 

                1015     363       2
 
EarI CTCTTCn'nnn_

Cuts at:      0      41     482     542     632     680     692    1380 
   Size:         41     441      60      90      48      12     688

  Fragments arranged by size: 

                 688     441      90      60      48      41      12
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     701     817     943    1106    1380 
   Size:        112     589     116     126     163     274

  Fragments arranged by size: 

                 589     274     163     126     116     112
 
EcoRI G'AATT_C

Cuts at:      0     716    1380 
   Size:        716     664
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     841    1380 
   Size:        841     539
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     229     261     443     475    1380 
   Size:        229      32     182      32     905

  Fragments arranged by size: 

                 905     229     182      32      32
 
HincII GTy'rAC

Cuts at:      0     905    1380 
   Size:        905     475
 
HindIII A'AGCT_T

Cuts at:      0     349     823    1334    1380 
   Size:        349     474     511      46

  Fragments arranged by size: 

                 511     474     349      46
 
MfeI C'AATT_G

Cuts at:      0    1319    1380 
   Size:       1319      61
 
MslI CAynn'nnrTG

Cuts at:      0     584    1380 
   Size:        584     796
 
MspA1I CmG'CkG

Cuts at:      0     112     157    1380 
   Size:        112      45    1223

  Fragments arranged by size: 

                1223     112      45
 
NheI G'CTAG_C

Cuts at:      0     933    1380 
   Size:        933     447
 
SfcI C'TryA_G

Cuts at:      0     482     957    1380 
   Size:        482     475     423

  Fragments arranged by size: 

                 482     475     423
 
SmlI C'TyrA_G

Cuts at:      0    1351    1380 
   Size:       1351      29
 
SnaBI TAC'GTA

Cuts at:      0    1189    1380 
   Size:       1189     191
 
SspI AAT'ATT

Cuts at:      0     886    1380 
   Size:        886     494
 
StyI C'CwwG_G

Cuts at:      0    1097    1380 
   Size:       1097     283
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     126     158     210     242    1380 
   Size:        126      32      52      32    1138

  Fragments arranged by size: 

                1138     126      52      32      32
 
XmnI GAAnn'nnTTC

Cuts at:      0    1091    1380 
   Size:       1091     289
 
ZraI GAC'GTC

Cuts at:      0     454    1380 
   Size:        454     926
 

 Enzymes that do cut:

    AatII     AccI     AclI     AcuI    AlwNI     ApoI     AvaI    BamHI
    BanII     BbsI     BmtI     BpmI    BpuEI    BsaAI    BsaBI    BsaHI
    BsaXI    BseRI    BseYI    BsmBI Bsp1286I    BspHI    BsrBI    BstYI
   Bsu36I     DraI     EaeI     EarI  Eco57MI    EcoRI     FalI    Hin4I
   HincII  HindIII     MfeI     MslI   MspA1I     NheI     SfcI     SmlI
    SnaBI     SspI     StyI     TstI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI   Bpu10I
     BsaI    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI
    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV
     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MluI
     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SpeI     SphI     SrfI     StuI     SwaI    TaqII
     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com