Restriction map for pF1KE0934
##################################
# NO CUT ENZYMES (  157 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspHI       AspI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BlnI        BlpI        BpmI        Bpu1102I    BsaAI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsmBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       FauI        FbaI        FseI        FspI        
HaeII       HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MaeII       MfeI        MluI        MluNI       
MroI        MscI        MunI        MvnI        NaeI        
NarI        NcoI        NgoMIV      NotI        NruI        
NspI        NspV        PacI        PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     Van91I      XbaI        XcmI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AhdI          601  GACATTTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         601  GACATTTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         1141  CTCGAG               C^[CT]CG[GA]G
AvaII         340  GGTCC                G^G[AT]CC
BanII         921  GGGCTC               G[GA]GC[CT]^C
BbvI          439  GCTGC                GCAGC(8/12)
BcnI         1177  CCCGG                CC^[GC]GG
BglII        1032  AGATCT               A^GATCT
BmyI          921  GGGCTC               G[AGT]GC[ACT]^C
BsaBI         661  GATATGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI         984  CGTACG               C^GTACG
BsiYI           6  CCACTCGCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI            6  CCACTCGCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI        1175  GTCCC                GGGAC(10/14)
BsmI          405  GCATTC               GAATGC(1/-1)
BsoBI        1141  CTCGAG               C^[CT]CG[GA]G
Bsp1286I      921  GGGCTC               G[AGT]GC[ACT]^C
BspMI        1074  ACCTGC               ACCTGC(4/8)
BsrFI          12  GCCGGT               [GA]^CCGG[CT]
BsrI          321  ACTGG                ACTGG(1/-1)
BstXI          24  CCAAGTGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI           84  GCGC                 GCG^C
Cfr10I         12  GCCGGT               [GA]^CCGG[CT]
Eam1105I      601  GACATTTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I       638  CCAAGG               C^C[AT][AT]GG
EcoT22I       664  ATGCAT               ATGCA^T
HaeIII          3  GGCC                 GG^CC
HhaI           84  GCGC                 GCG^C
HinP1I         84  GCGC                 G^CGC
MamI          661  GATATGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI          895  CACAGTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        502  CAGCTG               C[AC]G^C[GT]G
NciI         1177  CCCGG                CC^[GC]GG
NdeI          239  CATATG               CA^TATG
NheI         1114  GCTAGC               G^CTAGC
NlaIII        369  CATG                 CATG^
NlaIV         558  GGCTCC               GG[ACGT]^[ACGT]CC
NsiI          664  ATGCAT               ATGCA^T
PaeR7I       1141  CTCGAG               C^TCGAG
PvuII         502  CAGCTG               CAG^CTG
SfcI          788  CTACAG               C^T[GA][CT]AG
SgrAI          11  CGCCGGTG             C[GA]^CCGG[CT]G
StyI          638  CCAAGG               C^C[AT][AT]GG
TseI          439  GCTGC                G^C[AT]GC
Tsp45I        230  GTGAC                ^GT[GC]AC
XhoI         1141  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AciI         1169  CCGC                 CCGC(-3/-1)
              308  GCGG                
BbsI          193  GAAGAC               GAAGAC(2/6)
              837  GAAGAC              
BfaI          634  CTAG                 C^TAG
             1115  CTAG                
BpuAI         193  GAAGAC               GAAGAC(2/6)
              837  GAAGAC              
BsmAI         428  GAGAC                GTCTC(1/5)
              722  GAGAC               
BstNI          31  CCTGG                CC^[AT]GG
              303  CCTGG               
Cac8I         757  GCTTGC               GC[ACGT]^[ACGT]GC
             1114  GCTAGC              
Cfr13I          3  GGCCC                G^G[ACGT]CC
              340  GGTCC               
DraI          267  TTTAAA               TTT^AAA
              384  TTTAAA              
EcoRII         31  CCTGG                ^CC[AT]GG
              303  CCTGG               
Fnu4HI        439  GCTGC                GC^[ACGT]GC
             1168  GCCGC               
HapII          13  CCGG                 C^CGG
             1178  CCGG                
HpaII          13  CCGG                 C^CGG
             1178  CCGG                
HphI         1197  GGTGA                GGTGA(8/7)
              423  TCACC               
ItaI          439  GCTGC                GC^[ACGT]GC
             1168  GCCGC               
MaeI          634  CTAG                 C^TAG
             1115  CTAG                
MaeIII        230  GTGAC                ^GT[ACGT]AC
              244  GTAAC               
MspI           13  CCGG                 C^CGG
             1178  CCGG                
MvaI           31  CCTGG                CC^[AT]GG
              303  CCTGG               
MwoI           30  GCCTGGGATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1162  GCTGAAGCCGC         
PleI          589  GACTC                GAGTC(4/5)
              222  GAGTC               
Sau96I          3  GGCCC                G^G[ACGT]CC
              340  GGTCC               
TaqI          333  TCGA                 T^CGA
             1142  TCGA                
TspRI         320  CACTG                CA[GC]TG[ACGT][ACGT]^
              897  CAGTG               
TthHB8I       333  TCGA                 T^CGA
             1142  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
Asp700I     BsaJI       BssKI       BstF5I      BstYI       
DdeI        DpnII       FokI        HinfI       MboI        
MboII       MflI        MnlI        MseI        NdeII       
RsaI        Sau3AI      ScrFI       SfaNI       SmlI        
TfiI        Tru9I       Tsp509I     XhoII       XmnI        
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