Restriction map for pF1KE0934
 (Linear) (Six-base) MAPSORT of: KIEE0934.seq  Check: 7610  from: 1  to: 1248



KIEE0934.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1083    1248 
   Size:       1083     165
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     584    1184    1248 
   Size:        584     600      64

  Fragments arranged by size: 

                 600     584      64
 
AhdI GACnn_n'nnGTC

Cuts at:      0     606    1248 
   Size:        606     642
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     164    1248 
   Size:        164    1084
 
ApoI r'AATT_y

Cuts at:      0     380     419     814     828    1216    1248 
   Size:        380      39     395      14     388      32

  Fragments arranged by size: 

                 395     388     380      39      32      14
 
AvaI C'yCGr_G

Cuts at:      0    1141    1248 
   Size:       1141     107
 
BanII G_rGCy'C

Cuts at:      0     925    1248 
   Size:        925     323
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     200     844    1248 
   Size:        200     644     404

  Fragments arranged by size: 

                 644     404     200
 
BfrBI ATG'CAT

Cuts at:      0     666    1248 
   Size:        666     582
 
BglII A'GATC_T

Cuts at:      0    1032    1248 
   Size:       1032     216
 
BmtI G_CTAG'C

Cuts at:      0    1118    1248 
   Size:       1118     130
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     240    1248 
   Size:        190      50    1008

  Fragments arranged by size: 

                1008     190      50
 
BsaBI GATnn'nnATC

Cuts at:      0     665    1248 
   Size:        665     583
 
BseYI C'CCAG_C

Cuts at:      0     137    1248 
   Size:        137    1111
 
BsiWI C'GTAC_G

Cuts at:      0     984    1248 
   Size:        984     264
 
BsmI GAATG_Cn'

Cuts at:      0     405    1248 
   Size:        405     843
 
Bsp1286I G_dGCh'C

Cuts at:      0     925    1248 
   Size:        925     323
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1083    1248 
   Size:       1083     165
 
BsrFI r'CCGG_y

Cuts at:      0      12    1248 
   Size:         12    1236
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      31    1248 
   Size:         31    1217
 
BstYI r'GATC_y

Cuts at:      0     324     771    1032    1248 
   Size:        324     447     261     216

  Fragments arranged by size: 

                 447     324     261     216
 
DraI TTT'AAA

Cuts at:      0     269     386    1248 
   Size:        269     117     862

  Fragments arranged by size: 

                 862     269     117
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     584    1184    1248 
   Size:        584     600      64

  Fragments arranged by size: 

                 600     584      64
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     592     624     831     863     892     919     924     951 
   Size:        592      32     207      32      29      27       5      27

Cuts at:    951    1248 
   Size:        297

  Fragments arranged by size: 

                 592     297     207      32      32      29      27      27
                   5
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     699     733    1248 
   Size:        699      34     515

  Fragments arranged by size: 

                 699     515      34
 
MslI CAynn'nnrTG

Cuts at:      0     899    1248 
   Size:        899     349
 
MspA1I CmG'CkG

Cuts at:      0     504    1248 
   Size:        504     744
 
NdeI CA'TA_TG

Cuts at:      0     240    1248 
   Size:        240    1008
 
NheI G'CTAG_C

Cuts at:      0    1114    1248 
   Size:       1114     134
 
NsiI A_TGCA'T

Cuts at:      0     668    1248 
   Size:        668     580
 
PvuII CAG'CTG

Cuts at:      0     504    1248 
   Size:        504     744
 
SfcI C'TryA_G

Cuts at:      0     788    1248 
   Size:        788     460
 
SgrAI Cr'CCGG_yG

Cuts at:      0      12    1248 
   Size:         12    1236
 
SmlI C'TyrA_G

Cuts at:      0     169     219    1141    1248 
   Size:        169      50     922     107

  Fragments arranged by size: 

                 922     169     107      50
 
StyI C'CwwG_G

Cuts at:      0     638    1248 
   Size:        638     610
 
TatI w'GTAC_w

Cuts at:      0    1011    1248 
   Size:       1011     237
 
XhoI C'TCGA_G

Cuts at:      0    1141    1248 
   Size:       1141     107
 
XmnI GAAnn'nnTTC

Cuts at:      0     197     569    1217    1248 
   Size:        197     372     648      31

  Fragments arranged by size: 

                 648     372     197      31
 

 Enzymes that do cut:

     AarI     AcuI     AhdI     AlfI     ApoI     AvaI    BanII     BbsI
    BfrBI    BglII     BmtI    BpuEI    BsaBI    BseYI    BsiWI     BsmI
 Bsp1286I    BspMI    BsrFI    BstXI    BstYI     DraI  Eco57MI    Hin4I
     MmeI     MslI   MspA1I     NdeI     NheI     NsiI    PvuII     SfcI
    SgrAI     SmlI     StyI     TatI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI  BsiHKAI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI   NgoMIV
     NotI     NruI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI
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