Restriction map for pF1KE0834
##################################
# NO CUT ENZYMES (  160 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BbeI        BbrPI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       BspDI       BspEI       BspLU11I    BsrDI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Cac8I       CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRI       EcoRV       FbaI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HpaII       KasI        KspI        
MaeII       MamI        MfeI        MluI        MroI        
MseI        MspI        MunI        MvnI        MwoI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NotI        NruI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        TaqI        Tru9I       Tth111I     TthHB8I     
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Acc65I        625  GGTACC               G^GTACC
AocI          543  CCTGAGG              CC^T[ACGT]AGG
Asp700I        17  GAATTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       625  GGTACC               G^GTACC
AvaII         307  GGTCC                G^G[AT]CC
BamHI         548  GGATCC               G^GATCC
BanI          625  GGTACC               G^G[CT][GA]CC
BanII          99  GAGCCC               G[GA]GC[CT]^C
BbsI          490  GTCTTC               GAAGAC(2/6)
BbvCI          54  CCTCAGC              CCTCAGC(-5/-2)
BfaI          298  CTAG                 C^TAG
BmyI           99  GAGCCC               G[AGT]GC[ACT]^C
BpmI          476  CTGGAG               CTGGAG(16/14)
BpuAI         490  GTCTTC               GAAGAC(2/6)
BseRI          87  CTCCTC               GAGGAG(10/8)
BsmAI         362  GTCTC                GTCTC(1/5)
Bsp1286I       99  GAGCCC               G[AGT]GC[ACT]^C
Bsp1407I      645  TGTACA               T^GTACA
BspHI         326  TCATGA               T^CATGA
BspMI          79  ACCTGC               ACCTGC(4/8)
BsrBI         552  CCGCTC               CCGCTC(-3/-3)
BsrGI         645  TGTACA               T^GTACA
BsrI          653  CCAGT                ACTGG(1/-1)
Bsu36I        543  CCTGAGG              CC^T[ACGT]AGG
DraII         306  GGGTCCC              [GA]G^G[ACGT]CC[CT]
DsaI          218  CCATGG               C^C[GA][CT]GG
Eco81I        543  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      306  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoT22I       353  ATGCAT               ATGCA^T
FauI           26  CCCGC                CCCGC(4/6)
HphI          597  TCACC                GGTGA(8/7)
KpnI          625  GGTACC               GGTAC^C
MaeI          298  CTAG                 C^TAG
MspA1I        605  CAGCTG               C[AC]G^C[GT]G
NcoI          218  CCATGG               C^CATGG
NsiI          353  ATGCAT               ATGCA^T
PflMI         294  CCAGCTAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         306  GGGTCCC              [GA]G^G[AT]CC[CT]
PvuII         605  CAGCTG               CAG^CTG
RcaI          326  TCATGA               T^CATGA
SapI          590  GCTCTTC              GCTCTTC(1/4)
SfcI          735  CTACAG               C^T[GA][CT]AG
SmlI          741  CTTGAG               C^T[CT][GA]AG
SspBI         645  TGTACA               T^GTACA
Tsp45I        781  GTCAC                ^GT[GC]AC
Tsp509I        18  AATT                 ^AATT
Van91I        294  CCAGCTAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI           17  GAATTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AciI           27  CCGC                 CCGC(-3/-1)
              552  CCGC                
BalI          239  TGGCCA               TGG^CCA
              536  TGGCCA              
BbvI          165  GCAGC                GCAGC(8/12)
              193  GCAGC               
BssKI         443  CCAGG                ^CC[ACGT]GG
              669  CCTGG               
BstNI         443  CCAGG                CC^[AT]GG
              669  CCTGG               
BstYI         548  GGATCC               [GA]^GATC[CT]
              672  GGATCT              
Cfr13I        307  GGTCC                G^G[ACGT]CC
              376  GGCCC               
DpnII         549  GATC                 ^GATC
              673  GATC                
EaeI          239  TGGCCA               [CT]^GGCC[GA]
              536  TGGCCA              
EarI          591  CTCTTC               CTCTTC(1/4)
              129  GAAGAG              
EcoRII        443  CCAGG                ^CC[AT]GG
              669  CCTGG               
EcoT14I       150  CCTTGG               C^C[AT][AT]GG
              218  CCATGG              
Fnu4HI        165  GCAGC                GC^[ACGT]GC
              193  GCAGC               
ItaI          165  GCAGC                GC^[ACGT]GC
              193  GCAGC               
Ksp632I       591  CTCTTC               CTCTTC(1/4)
              129  GAAGAG              
MaeIII        248  GTAAC                ^GT[ACGT]AC
              781  GTCAC               
MboI          549  GATC                 ^GATC
              673  GATC                
MflI          548  GGATCC               [GA]^GATC[CT]
              672  GGATCT              
MluNI         239  TGGCCA               TGG^CCA
              536  TGGCCA              
MscI          239  TGGCCA               TGG^CCA
              536  TGGCCA              
MvaI          443  CCAGG                CC^[AT]GG
              669  CCTGG               
NdeII         549  GATC                 ^GATC
              673  GATC                
Sau3AI        549  GATC                 ^GATC
              673  GATC                
Sau96I        307  GGTCC                G^G[ACGT]CC
              376  GGCCC               
ScrFI         443  CCAGG                CC^[ACGT]GG
              669  CCTGG               
StyI          150  CCTTGG               C^C[AT][AT]GG
              218  CCATGG              
TfiI           22  GATTC                G^A[AT]TC
              711  GATTC               
TseI          165  GCAGC                G^C[AT]GC
              193  GCAGC               
XhoII         548  GGATCC               [GA]^GATC[CT]
              672  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AfaI        AluI        AlwI        BsaJI       BsiYI       
BslI        BsmFI       BstF5I      DdeI        FokI        
HaeIII      HinfI       MboII       MnlI        MslI        
NlaIII      NlaIV       PleI        RsaI        SfaNI       
TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com