Restriction map for pF1KE0834
 (Linear) (Six-base) MAPSORT of: KIEE0834.seq  Check: 9759  from: 1  to: 807



KIEE0834.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      88     807 
   Size:         88     719
 
Acc65I G'GTAC_C

Cuts at:      0     625     807 
   Size:        625     182
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     477     807 
   Size:        477     330
 
AleI CACnn'nnGTG

Cuts at:      0     706     807 
   Size:        706     101
 
BamHI G'GATC_C

Cuts at:      0     548     807 
   Size:        548     259
 
BanI G'GyrC_C

Cuts at:      0     625     807 
   Size:        625     182
 
BanII G_rGCy'C

Cuts at:      0     103     807 
   Size:        103     704
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     483     807 
   Size:        483     324
 
BbvCI CC'TCA_GC

Cuts at:      0      55     807 
   Size:         55     752
 
BfrBI ATG'CAT

Cuts at:      0     355     807 
   Size:        355     452
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     647     807 
   Size:        647     160
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     497     807 
   Size:        497     310
 
Bpu10I CC'TnA_GC

Cuts at:      0      55     807 
   Size:         55     752
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     762     807 
   Size:        762      45
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      78     807 
   Size:         78     729
 
Bsp1286I G_dGCh'C

Cuts at:      0     103     807 
   Size:        103     704
 
BspHI T'CATG_A

Cuts at:      0     326     807 
   Size:        326     481
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      88     807 
   Size:         88     719
 
BsrBI CCG'CTC

Cuts at:      0     554     807 
   Size:        554     253
 
BsrGI T'GTAC_A

Cuts at:      0     645     807 
   Size:        645     162
 
BstYI r'GATC_y

Cuts at:      0     548     672     807 
   Size:        548     124     135

  Fragments arranged by size: 

                 548     135     124
 
Bsu36I CC'TnA_GG

Cuts at:      0     544     807 
   Size:        544     263
 
BtgI C'CryG_G

Cuts at:      0     218     807 
   Size:        218     589
 
BtsI GCAGTG_nn'

Cuts at:      0     175     713     807 
   Size:        175     538      94

  Fragments arranged by size: 

                 538     175      94
 
EaeI y'GGCC_r

Cuts at:      0     239     536     807 
   Size:        239     297     271

  Fragments arranged by size: 

                 297     271     239
 
EarI CTCTTCn'nnn_

Cuts at:      0     124     597     807 
   Size:        124     473     210

  Fragments arranged by size: 

                 473     210     124
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     477     497     807 
   Size:        477      20     310

  Fragments arranged by size: 

                 477     310      20
 
EcoO109I rG'GnC_Cy

Cuts at:      0     307     807 
   Size:        307     500
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     768     800     807 
   Size:        768      32       7

  Fragments arranged by size: 

                 768      32       7
 
KpnI G_GTAC'C

Cuts at:      0     629     807 
   Size:        629     178
 
MscI TGG'CCA

Cuts at:      0     241     538     807 
   Size:        241     297     269

  Fragments arranged by size: 

                 297     269     241
 
MslI CAynn'nnrTG

Cuts at:      0      40     532     653     706     807 
   Size:         40     492     121      53     101

  Fragments arranged by size: 

                 492     121     101      53      40
 
MspA1I CmG'CkG

Cuts at:      0     607     807 
   Size:        607     200
 
NcoI C'CATG_G

Cuts at:      0     218     807 
   Size:        218     589
 
NsiI A_TGCA'T

Cuts at:      0     357     807 
   Size:        357     450
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     300     807 
   Size:        300     507
 
PpuMI rG'GwC_Cy

Cuts at:      0     307     807 
   Size:        307     500
 
PvuII CAG'CTG

Cuts at:      0     607     807 
   Size:        607     200
 
SanDI GG'GwC_CC

Cuts at:      0     307     807 
   Size:        307     500
 
SapI GCTCTTCn'nnn_

Cuts at:      0     597     807 
   Size:        597     210
 
SfcI C'TryA_G

Cuts at:      0     735     807 
   Size:        735      72
 
SmlI C'TyrA_G

Cuts at:      0     741     807 
   Size:        741      66
 
StyI C'CwwG_G

Cuts at:      0     150     218     807 
   Size:        150      68     589

  Fragments arranged by size: 

                 589     150      68
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      54     807 
   Size:         54     753
 
TatI w'GTAC_w

Cuts at:      0      44     645     807 
   Size:         44     601     162

  Fragments arranged by size: 

                 601     162      44
 
XmnI GAAnn'nnTTC

Cuts at:      0      21     807 
   Size:         21     786
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AleI    BamHI     BanI    BanII     BbsI
    BbvCI    BfrBI     BmrI     BpmI   Bpu10I    BpuEI    BseRI Bsp1286I
    BspHI    BspMI    BsrBI    BsrGI    BstYI   Bsu36I     BtgI     BtsI
     EaeI     EarI  Eco57MI EcoO109I    Hin4I     KpnI     MscI     MslI
   MspA1I     NcoI     NsiI    PflMI    PpuMI    PvuII    SanDI     SapI
     SfcI     SmlI     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BbeI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     MfeI     MluI     MmeI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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