Restriction map for pF1KE0815
##################################
# NO CUT ENZYMES (  198 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BamHI       BanII       
BbeI        BbrPI       BbvCI       BbvI        BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmI        BsoBI       Bsp1286I    BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DdeI        DraI        
DraII       DraIII      DrdI        DsaI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HpaI        HpaII       
KasI        KpnI        Ksp632I     KspI        MaeI        
MamI        MfeI        MflI        MluI        MroI        
MseI        MspA1I      MspI        MunI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    StuI        StyI        SwaI        
TaqI        Tru9I       TseI        Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AciI          198  CCGC                 CCGC(-3/-1)
AcsI           18  AAATTT               [GA]^AATT[CT]
AfaI            3  GTAC                 GT^AC
AlwNI         509  CAGAGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI           18  AAATTT               [GA]^AATT[CT]
BalI           65  TGGCCA               TGG^CCA
BanI          445  GGCACC               G^G[CT][GA]CC
BsmFI         361  GTCCC                GGGAC(10/14)
Bsp1407I        2  TGTACA               T^GTACA
BsrGI           2  TGTACA               T^GTACA
BstF5I        500  GGATG                GGATG(2/0)
Cfr13I        325  GGCCC                G^G[ACGT]CC
EaeI           65  TGGCCA               [CT]^GGCC[GA]
EcoNI         173  CCTTTGACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I       216  ATGCAT               ATGCA^T
Fnu4HI        197  GCCGC                GC^[ACGT]GC
FokI          500  GGATG                GGATG(9/13)
HindIII       527  AAGCTT               A^AGCTT
ItaI          197  GCCGC                GC^[ACGT]GC
MaeII         207  ACGT                 A^CGT
MaeIII        562  GTCAC                ^GT[ACGT]AC
MluNI          65  TGGCCA               TGG^CCA
MscI           65  TGGCCA               TGG^CCA
NlaIV         445  GGCACC               GG[ACGT]^[ACGT]CC
NsiI          216  ATGCAT               ATGCA^T
RsaI            3  GTAC                 GT^AC
Sau96I        325  GGCCC                G^G[ACGT]CC
SspBI           2  TGTACA               T^GTACA
SspI          331  AATATT               AAT^ATT
Tsp45I        562  GTCAC                ^GT[GC]AC
TspRI         240  CACTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AlwI          355  GATCC                GGATC(4/5)
              607  GGATC               
BbsI          388  GAAGAC               GAAGAC(2/6)
              433  GTCTTC              
BpuAI         388  GAAGAC               GAAGAC(2/6)
              433  GTCTTC              
BsiYI         173  CCTTTGACAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              285  CCTAACTCTGG         
BslI          173  CCTTTGACAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              285  CCTAACTCTGG         
BspHI         101  TCATGA               T^CATGA
              221  TCATGA              
BsrI          241  ACTGG                ACTGG(1/-1)
              619  CCAGT               
BssKI          30  CCTGG                ^CC[ACGT]GG
              604  CCTGG               
BstNI          30  CCTGG                CC^[AT]GG
              604  CCTGG               
Cac8I          63  GCTGGC               GC[ACGT]^[ACGT]GC
              514  GCTGGC              
DpnII         355  GATC                 ^GATC
              608  GATC                
EcoRII         30  CCTGG                ^CC[AT]GG
              604  CCTGG               
HinfI         297  GATTC                G^A[ACGT]TC
              483  GATTC               
MboI          355  GATC                 ^GATC
              608  GATC                
MslI          260  CATGGTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              585  CATGGTGATG          
MvaI           30  CCTGG                CC^[AT]GG
              604  CCTGG               
NdeII         355  GATC                 ^GATC
              608  GATC                
RcaI          101  TCATGA               T^CATGA
              221  TCATGA              
Sau3AI        355  GATC                 ^GATC
              608  GATC                
ScrFI          30  CCTGG                CC^[ACGT]GG
              604  CCTGG               
TfiI          297  GATTC                G^A[AT]TC
              483  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (    8 enzymes) #
#########################################

AluI        HaeIII      HphI        MboII       MnlI        
NlaIII      SfaNI       Tsp509I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com