Restriction map for pF1KB9953
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AlwI        ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvrII       BamHI       BbrPI       BbvCI       
BcgI        BciVI       BfaI        BfrI        BglII       
BlnI        BsaBI       BsaI        BseAI       BsiEI       
BsiWI       BsmI        BspDI       BspEI       BspLU11I    
BsrDI       BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstYI       BstZ17I     ClaI        DraI        
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco52I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        HincII      HindII      HindIII     
HpaI        KpnI        Ksp632I     KspI        MaeI        
MamI        MfeI        MflI        MroI        MseI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        SacII       SalI        SapI        
ScaI        SfiI        SfuI        SgrAI       SpeI        
SphI        SspI        SwaI        TfiI        Tru9I       
Tsp509I     Tth111I     XbaI        XcmI        XhoII       
XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AflIII        323  ACGCGT               A^C[GA][CT]GT
AocI         1521  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       527  AGCGCT               AGC^GCT
BalI          554  TGGCCA               TGG^CCA
BbsI          304  GTCTTC               GAAGAC(2/6)
BclI          314  TGATCA               T^GATCA
BpuAI         304  GTCTTC               GAAGAC(2/6)
BsaWI         947  TCCGGT               [AT]^CCGG[AT]
BsmAI        1255  GAGAC                GTCTC(1/5)
BsmBI        1255  GAGACG               CGTCTC(1/5)
BssHII       1515  GCGCGC               G^CGCGC
BstXI         557  CCATGCGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1521  CCTCAGG              CC^T[ACGT]AGG
CpoI          208  CGGACCG              CG^G[AT]CCG
DpnII         315  GATC                 ^GATC
Eco47III      527  AGCGCT               AGC^GCT
Eco81I       1521  CCTCAGG              CC^T[ACGT]AGG
EcoT14I       710  CCATGG               C^C[AT][AT]GG
FbaI          314  TGATCA               T^GATCA
MboI          315  GATC                 ^GATC
MluI          323  ACGCGT               A^CGCGT
MluNI         554  TGGCCA               TGG^CCA
MscI          554  TGGCCA               TGG^CCA
MslI          571  CACCAGAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI          710  CCATGG               C^CATGG
NdeII         315  GATC                 ^GATC
PaeR7I        238  CTCGAG               C^TCGAG
RsrII         208  CGGACCG              CG^G[AT]CCG
Sau3AI        315  GATC                 ^GATC
SbfI         1122  CCTGCAGG             CCTGCA^GG
SexAI         158  ACCTGGT              A^CC[AT]GGT
SnaBI         995  TACGTA               TAC^GTA
Sse8387I     1122  CCTGCAGG             CCTGCA^GG
StyI          710  CCATGG               C^C[AT][AT]GG
XhoI          238  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

BanI          589  GGCGCC               G^G[CT][GA]CC
             1478  GGCGCC              
BbeI          589  GGCGCC               GGCGC^C
             1478  GGCGCC              
BglI          215  GCCACAAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1361  GCCTTGCCGGC         
BlpI          144  GCTCAGC              GC^T[ACGT]AGC
              408  GCTGAGC             
Bpu1102I      144  GCTCAGC              GC^T[ACGT]AGC
              408  GCTGAGC             
BsaAI         226  TACGTG               [CT]AC^GT[GA]
              995  TACGTA              
Bsp1407I      269  TGTACA               T^GTACA
             1013  TGTACA              
BspHI         368  TCATGA               T^CATGA
              650  TCATGA              
BsrBI        1066  GAGCGG               CCGCTC(-3/-3)
             1471  GAGCGG              
BsrGI         269  TGTACA               T^GTACA
             1013  TGTACA              
BstAPI        864  GCAGGCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1413  GCAGGTTTTGC         
CelII         144  GCTCAGC              GC^T[ACGT]AGC
              408  GCTGAGC             
KasI          589  GGCGCC               G^GCGCC
             1478  GGCGCC              
NarI          589  GGCGCC               GG^CGCC
             1478  GGCGCC              
PflMI         840  CCAGGTACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1323  CCAGGACCTGG         
PshAI         360  GACTGAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1257  GACGCTCGTC          
RcaI          368  TCATGA               T^CATGA
              650  TCATGA              
SfoI          589  GGCGCC               GGC^GCC
             1478  GGCGCC              
SmaI          776  CCCGGG               CCC^GGG
             1115  CCCGGG              
SspBI         269  TGTACA               T^GTACA
             1013  TGTACA              
Van91I        840  CCAGGTACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1323  CCAGGACCTGG         
XmaCI         776  CCCGGG               C^CCGGG
             1115  CCCGGG              
XmaI          776  CCCGGG               C^CCGGG
             1115  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       AspHI       AvaI        AvaII       AviII       
BanII       BbvI        BcnI        BmyI        BpmI        
BsaHI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BspMI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DraII       DsaI        EaeI        
EcoNI       EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboII       MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NciI        NgoMIV      
NlaIII      NlaIV       PleI        PpuMI       PstI        
PvuII       RsaI        SacI        Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StuI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     
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