Restriction map for pF1KB9953
 (Linear) (Six-base) MAPSORT of: KIBB9953.seq  Check: 9567  from: 1  to: 1608



KIBB9953.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     129    1140    1182    1608 
   Size:        129    1011      42     426

  Fragments arranged by size: 

                1011     426     129      42
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     715    1608 
   Size:        715     893
 
AfeI AGC'GCT

Cuts at:      0     529    1608 
   Size:        529    1079
 
AflIII A'CryG_T

Cuts at:      0     323    1608 
   Size:        323    1285
 
AleI CACnn'nnGTG

Cuts at:      0     575    1608 
   Size:        575    1033
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1168    1608 
   Size:       1168     440
 
AlwNI CAG_nnn'CTG

Cuts at:      0     354     396     735     789     846     870     987    1218 
   Size:        354      42     339      54      57      24     117     231

Cuts at:   1218    1251    1329    1608 
   Size:         33      78     279

  Fragments arranged by size: 

                 354     339     279     231     117      78      57      54
                  42      33      24
 
AvaI C'yCGr_G

Cuts at:      0     238     776    1115    1462    1608 
   Size:        238     538     339     347     146

  Fragments arranged by size: 

                 538     347     339     238     146
 
BanI G'GyrC_C

Cuts at:      0     589    1478    1608 
   Size:        589     889     130

  Fragments arranged by size: 

                 889     589     130
 
BanII G_rGCy'C

Cuts at:      0     254     415     524    1273    1313    1381    1606    1608 
   Size:        254     161     109     749      40      68     225       2

  Fragments arranged by size: 

                 749     254     225     161     109      68      40       2
            
 
BbeI G_GCGC'C

Cuts at:      0     593    1482    1608 
   Size:        593     889     126

  Fragments arranged by size: 

                 889     593     126
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     297    1608 
   Size:        297    1311
 
BclI T'GATC_A

Cuts at:      0     314    1608 
   Size:        314    1294
 
BglI GCCn_nnn'nGGC

Cuts at:      0     221    1367    1608 
   Size:        221    1146     241

  Fragments arranged by size: 

                1146     241     221
 
BlpI GC'TnA_GC

Cuts at:      0     145     409    1608 
   Size:        145     264    1199

  Fragments arranged by size: 

                1199     264     145
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     268     643     754     811     868    1273    1351 
   Size:         61     207     375     111      57      57     405      78

Cuts at:   1351    1608 
   Size:        257

  Fragments arranged by size: 

                 405     375     257     207     111      78      61      57
                  57
 
Bpu10I CC'TnA_GC

Cuts at:      0     700    1237    1267    1444    1608 
   Size:        700     537      30     177     164

  Fragments arranged by size: 

                 700     537     177     164      30
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     475     508     622    1608 
   Size:        475      33     114     986

  Fragments arranged by size: 

                 986     475     114      33
 
BsaAI yAC'GTr

Cuts at:      0     228     997    1608 
   Size:        228     769     611

  Fragments arranged by size: 

                 769     611     228
 
BsaHI Gr'CG_yC

Cuts at:      0     590     942    1479    1608 
   Size:        590     352     537     129

  Fragments arranged by size: 

                 590     537     352     129
 
BsaWI w'CCGG_w

Cuts at:      0     947    1608 
   Size:        947     661
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     724     754    1608 
   Size:        724      30     854

  Fragments arranged by size: 

                 854     724      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     148     511     553     595     865     973    1066    1108 
   Size:        148     363      42      42     270     108      93      42

Cuts at:   1108    1570    1608 
   Size:        462      38

  Fragments arranged by size: 

                 462     363     270     148     108      93      42      42
                  42      38
 
BseYI C'CCAG_C

Cuts at:      0     724    1161    1340    1608 
   Size:        724     437     179     268

  Fragments arranged by size: 

                 724     437     268     179
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     409     502     802    1084    1387    1608 
   Size:        409      93     300     282     303     221

  Fragments arranged by size: 

                 409     303     300     282     221      93
 
BsiHKAI G_wGCw'C

Cuts at:      0     146     191     254     415     524    1273    1313    1608 
   Size:        146      45      63     161     109     749      40     295

  Fragments arranged by size: 

                 749     295     161     146     109      63      45      40
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1249    1608 
   Size:       1249     359
 
Bsp1286I G_dGCh'C

Cuts at:      0     146     191     254     415     524    1273    1313    1381 
   Size:        146      45      63     161     109     749      40      68

Cuts at:   1381    1606    1608 
   Size:        225       2

  Fragments arranged by size: 

                 749     225     161     146     109      68      63      45
                  40       2
 
BspHI T'CATG_A

Cuts at:      0     368     650    1608 
   Size:        368     282     958

  Fragments arranged by size: 

                 958     368     282
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     107     129     623    1140    1182    1404    1608 
   Size:        107      22     494     517      42     222     204

  Fragments arranged by size: 

                 517     494     222     204     107      42      22
 
BsrBI CCG'CTC

Cuts at:      0    1068    1473    1608 
   Size:       1068     405     135

  Fragments arranged by size: 

                1068     405     135
 
BsrFI r'CCGG_y

Cuts at:      0     211     294     508    1366    1608 
   Size:        211      83     214     858     242

  Fragments arranged by size: 

                 858     242     214     211      83
 
BsrGI T'GTAC_A

Cuts at:      0     269    1013    1608 
   Size:        269     744     595

  Fragments arranged by size: 

                 744     595     269
 
BssHII G'CGCG_C

Cuts at:      0    1515    1608 
   Size:       1515      93
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     870    1419    1608 
   Size:        870     549     189

  Fragments arranged by size: 

                 870     549     189
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     564    1608 
   Size:        564    1044
 
Bsu36I CC'TnA_GG

Cuts at:      0    1522    1608 
   Size:       1522      86
 
BtgI C'CryG_G

Cuts at:      0     710    1223    1574    1608 
   Size:        710     513     351      34

  Fragments arranged by size: 

                 710     513     351      34
 
BtsI GCAGTG_nn'

Cuts at:      0     112     988    1608 
   Size:        112     876     620

  Fragments arranged by size: 

                 876     620     112
 
EaeI y'GGCC_r

Cuts at:      0     213     554    1220    1608 
   Size:        213     341     666     388

  Fragments arranged by size: 

                 666     388     341     213
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     913    1189    1608 
   Size:        913     276     419

  Fragments arranged by size: 

                 913     419     276
 
EcoICRI GAG'CTC

Cuts at:      0     252     413     522    1271    1311    1608 
   Size:        252     161     109     749      40     297

  Fragments arranged by size: 

                 749     297     252     161     109      40
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     268     643     715     754     811     868    1273 
   Size:         61     207     375      72      39      57      57     405

Cuts at:   1273    1351    1608 
   Size:         78     257

  Fragments arranged by size: 

                 405     375     257     207      78      72      61      57
                  57      39
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     652     769     913     925    1608 
   Size:        652     117     144      12     683

  Fragments arranged by size: 

                 683     652     144     117      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0     114     393     762     918    1119    1301    1326    1608 
   Size:        114     279     369     156     201     182      25     282

  Fragments arranged by size: 

                 369     282     279     201     182     156     114      25
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1378    1608 
   Size:       1378     230
 
FspI TGC'GCA

Cuts at:      0     463     913    1144    1608 
   Size:        463     450     231     464

  Fragments arranged by size: 

                 464     463     450     231
 
HaeII r_GCGC'y

Cuts at:      0     531     593     871    1482    1608 
   Size:        531      62     278     611     126

  Fragments arranged by size: 

                 611     531     278     126      62
 
KasI G'GCGC_C

Cuts at:      0     589    1478    1608 
   Size:        589     889     130

  Fragments arranged by size: 

                 889     589     130
 
MluI A'CGCG_T

Cuts at:      0     323    1608 
   Size:        323    1285
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1298    1608 
   Size:       1298     310
 
MscI TGG'CCA

Cuts at:      0     556    1608 
   Size:        556    1052
 
MslI CAynn'nnrTG

Cuts at:      0     575    1608 
   Size:        575    1033
 
MspA1I CmG'CkG

Cuts at:      0     207     267     351     621     720    1188    1251    1451 
   Size:        207      60      84     270      99     468      63     200

Cuts at:   1451    1608 
   Size:        157

  Fragments arranged by size: 

                 468     270     207     200     157      99      84      63
                  60
 
NaeI GCC'GGC

Cuts at:      0     296     510    1368    1608 
   Size:        296     214     858     240

  Fragments arranged by size: 

                 858     296     240     214
 
NarI GG'CG_CC

Cuts at:      0     590    1479    1608 
   Size:        590     889     129

  Fragments arranged by size: 

                 889     590     129
 
NcoI C'CATG_G

Cuts at:      0     710    1608 
   Size:        710     898
 
NgoMIV G'CCGG_C

Cuts at:      0     294     508    1366    1608 
   Size:        294     214     858     242

  Fragments arranged by size: 

                 858     294     242     214
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     846    1329    1608 
   Size:        846     483     279

  Fragments arranged by size: 

                 846     483     279
 
PfoI T'CCnGG_A

Cuts at:      0      38     191     803    1608 
   Size:         38     153     612     805

  Fragments arranged by size: 

                 805     612     153      38
 
PpuMI rG'GwC_Cy

Cuts at:      0     114     393     918    1301    1326    1608 
   Size:        114     279     525     383      25     282

  Fragments arranged by size: 

                 525     383     282     279     114      25
 
PshAI GACnn'nnGTC

Cuts at:      0     364    1261    1608 
   Size:        364     897     347

  Fragments arranged by size: 

                 897     364     347
 
PstI C_TGCA'G

Cuts at:      0     206     380     620     752     983    1046    1127    1187 
   Size:        206     174     240     132     231      63      81      60

Cuts at:   1187    1193    1608 
   Size:          6     415

  Fragments arranged by size: 

                 415     240     231     206     174     132      81      63
                  60       6
 
PvuII CAG'CTG

Cuts at:      0     267     351     621    1188    1251    1608 
   Size:        267      84     270     567      63     357

  Fragments arranged by size: 

                 567     357     270     267      84      63
 
RsrII CG'GwC_CG

Cuts at:      0     209    1608 
   Size:        209    1399
 
SacI G_AGCT'C

Cuts at:      0     254     415     524    1273    1313    1608 
   Size:        254     161     109     749      40     295

  Fragments arranged by size: 

                 749     295     254     161     109      40
 
SbfI CC_TGCA'GG

Cuts at:      0    1127    1608 
   Size:       1127     481
 
SexAI A'CCwGG_T

Cuts at:      0     158    1608 
   Size:        158    1450
 
SfcI C'TryA_G

Cuts at:      0     202     376     616     748     979    1042    1123    1183 
   Size:        202     174     240     132     231      63      81      60

Cuts at:   1183    1189    1608 
   Size:          6     419

  Fragments arranged by size: 

                 419     240     231     202     174     132      81      63
                  60       6
 
SfoI GGC'GCC

Cuts at:      0     591    1480    1608 
   Size:        591     889     128

  Fragments arranged by size: 

                 889     591     128
 
SmaI CCC'GGG

Cuts at:      0     778    1117    1608 
   Size:        778     339     491

  Fragments arranged by size: 

                 778     491     339
 
SmlI C'TyrA_G

Cuts at:      0     238     490     523     637    1608 
   Size:        238     252      33     114     971

  Fragments arranged by size: 

                 971     252     238     114      33
 
SnaBI TAC'GTA

Cuts at:      0     997    1608 
   Size:        997     611
 
StuI AGG'CCT

Cuts at:      0     341     796    1442    1608 
   Size:        341     455     646     166

  Fragments arranged by size: 

                 646     455     341     166
 
StyI C'CwwG_G

Cuts at:      0     710    1608 
   Size:        710     898
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      57     172    1308    1354    1608 
   Size:         57     115    1136      46     254

  Fragments arranged by size: 

                1136     254     115      57      46
 
TatI w'GTAC_w

Cuts at:      0     269    1013    1608 
   Size:        269     744     595

  Fragments arranged by size: 

                 744     595     269
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     168     200     724     756    1608 
   Size:        168      32     524      32     852

  Fragments arranged by size: 

                 852     524     168      32      32
 
XhoI C'TCGA_G

Cuts at:      0     238    1608 
   Size:        238    1370
 
XmaI C'CCGG_G

Cuts at:      0     776    1115    1608 
   Size:        776     339     493

  Fragments arranged by size: 

                 776     493     339
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AleI     AlfI    AlwNI     AvaI
     BanI    BanII     BbeI     BbsI     BclI     BglI     BlpI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII   BstAPI    BstXI   Bsu36I     BtgI     BtsI     EaeI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FspI    HaeII
     KasI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV    PflMI     PfoI    PpuMI    PshAI     PstI    PvuII
    RsrII     SacI     SbfI    SexAI     SfcI     SfoI     SmaI     SmlI
    SnaBI     StuI     StyI    TaqII     TatI     TstI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AloI
     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI    BbvCI     BcgI    BciVI    BfrBI    BglII Bme1580I    BmgBI
     BmrI     BmtI     BplI     BsaI    BsaBI    BsiEI    BsiWI     BsmI
    BspEI    BsrDI    BssSI    BstBI   BstEII    BstYI  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI    EcoRI
    EcoRV     FseI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI
     PsrI     PvuI    SacII     SalI    SanDI     SapI     ScaI     SfiI
     SgfI    SgrAI     SpeI     SphI     SrfI     SspI     SwaI  Tth111I
     XbaI     XcmI     XmnI     ZraI
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