Restriction map for
pF1KB0408
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# NO CUT ENZYMES ( 97 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI ApoI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BbeI BciVI BclI
BfaI BfrI BglII BlnI BsaHI
BsaWI BseAI BsiWI BspDI BspEI
BspHI BspLU11I BsrDI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII DrdI Eam1105I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MfeI MluI MroI MunI NarI
NcoI NdeI NheI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI TaqI Tsp509I TthHB8I
XbaI XhoI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccI 1876 GTAGAC GT^[AC][GT]AC
AflIII 1505 ACGTGT A^C[GA][CT]GT
AocI 1454 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1682 AGCGCT AGC^GCT
ApaI 1414 GGGCCC GGGCC^C
AspI 1981 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1481 GGATCC G^GATCC
BglI 2289 GCCTGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1398 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2071 GAGACG CGTCTC(1/5)
BsmI 1565 GCATTC GAATGC(1/-1)
Bsp1407I 230 TGTACA T^GTACA
BsrBI 1272 CCGCTC CCGCTC(-3/-3)
BsrGI 230 TGTACA T^GTACA
BssHII 1162 GCGCGC G^CGCGC
Bsu36I 1454 CCTCAGG CC^T[ACGT]AGG
DsaI 281 CCGTGG C^C[GA][CT]GG
Eco47III 1682 AGCGCT AGC^GCT
Eco81I 1454 CCTCAGG CC^T[ACGT]AGG
MamI 1398 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 104 TTAA T^TAA
NaeI 348 GCCGGC GCC^GGC
NgoMIV 348 GCCGGC G^CCGGC
NotI 1268 GCGGCCGC GC^GGCCGC
NruI 2334 TCGCGA TCG^CGA
PflFI 1981 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
ScaI 140 AGTACT AGT^ACT
SexAI 1367 ACCAGGT A^CC[AT]GGT
SspBI 230 TGTACA T^GTACA
Tru9I 104 TTAA T^TAA
Tth111I 1981 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AfaI 141 GTAC GT^AC
231 GTAC
BcgI 1691 GCAAGAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1775 GCAAGGCCTTCG
BsaI 1312 GAGACC GGTCTC(1/5)
2368 GAGACC
BsrFI 348 GCCGGC [GA]^CCGG[CT]
2043 GCCGGT
BssSI 603 CACGAG CACGAG(-5/-1)
1002 CTCGTG
BstXI 1709 CCAGCCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2186 CCAACAGCCTGG
Cfr10I 348 GCCGGC [GA]^CCGG[CT]
2043 GCCGGT
EagI 1037 CGGCCG C^GGCCG
1269 CGGCCG
EclXI 1037 CGGCCG C^GGCCG
1269 CGGCCG
Eco52I 1037 CGGCCG C^GGCCG
1269 CGGCCG
EcoNI 207 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
492 CCTCAAGGAGG
EcoT14I 380 CCTTGG C^C[AT][AT]GG
893 CCAAGG
NspI 24 GCATGT [GA]CATG^[CT]
2202 GCATGT
PflMI 1221 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2223 CCAGGCACTGG
PpuMI 488 GGGACCT [GA]G^G[AT]CC[CT]
1796 GGGACCT
PshAI 2016 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2073 GACGGAAGTC
RsaI 141 GTAC GT^AC
231 GTAC
SmaI 566 CCCGGG CCC^GGG
851 CCCGGG
StyI 380 CCTTGG C^C[AT][AT]GG
893 CCAAGG
TfiI 1697 GATTC G^A[AT]TC
2210 GAATC
Van91I 1221 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2223 CCAGGCACTGG
XcmI 602 CCACGAGTGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2275 CCAGGTGGCACGTGG
XmaCI 566 CCCGGG C^CCGGG
851 CCCGGG
XmaI 566 CCCGGG C^CCGGG
851 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AluI Alw44I AlwI
AlwNI ApaLI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbsI BbvCI BbvI BcnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PmaCI PmlI
PstI PvuII SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI TseI Tsp45I TspRI XhoII
XmnI