Restriction map for pF1KB0405
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AvrII       BamHI       BanI        BbeI        
BbrPI       BcgI        BciVI       BclI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaHI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstZ17I     CelII       ClaI        CpoI        DraI        
DraIII      DrdI        EagI        EclXI       Eco47III    
Eco52I      EcoNI       EcoRV       EcoT22I     FbaI        
FseI        HgaI        Hin1I       HindIII     HpaI        
KasI        KpnI        KspI        MfeI        MluI        
MroI        MseI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        TaqI        
Tru9I       TthHB8I     XbaI        XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccI          805  GTCTAC               GT^[AC][GT]AC
AccII        1226  CGCG                 CG^CG
AcsI          613  GAATTC               [GA]^AATT[CT]
AhdI          466  GACGTTCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        1336  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1378  CCTGAGG              CC^T[ACGT]AGG
ApoI          613  GAATTC               [GA]^AATT[CT]
AspEI         466  GACGTTCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         344  GAGCAC               G[AT]GC[AT]^C
AspI          357  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         539  TGCGCA               TGC^GCA
BbsI          649  GAAGAC               GAAGAC(2/6)
BbvCI         309  CCTCAGC              CCTCAGC(-5/-2)
BglI         1202  GCCGGACGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         649  GAAGAC               GAAGAC(2/6)
BsaBI         972  GATCCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI       344  GAGCAC               G[AT]GC[AT]^C
BsmAI        1150  GAGAC                GTCTC(1/5)
BsmI          119  GAATGC               GAATGC(1/-1)
BsrDI         810  CATTGC               GCAATG(2/0)
BsrFI        1046  GCCGGT               [GA]^CCGG[CT]
BstEII        522  GGTCACC              G^GT[ACGT]ACC
BstPI         522  GGTCACC              G^GT[ACGT]ACC
BstUI        1226  CGCG                 CG^CG
Bsu36I       1378  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       1046  GCCGGT               [GA]^CCGG[CT]
DsaI          327  CCATGG               C^C[GA][CT]GG
Eam1105I      466  GACGTTCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1378  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       522  GGTCACC              G^GT[ACGT]ACC
EcoRI         613  GAATTC               G^AATTC
FspI          539  TGCGCA               TGC^GCA
HinfI        1099  GAATC                G^A[ACGT]TC
MaeII         467  ACGT                 A^CGT
MaeIII        523  GTCAC                ^GT[ACGT]AC
MamI          972  GATCCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI         1226  CGCG                 CG^CG
NcoI          327  CCATGG               C^CATGG
NspI          417  GCATGT               [GA]CATG^[CT]
PflFI         357  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SapI         1039  GAAGAGC              GCTCTTC(1/4)
StuI           11  AGGCCT               AGG^CCT
TfiI         1099  GAATC                G^A[AT]TC
Tsp45I        523  GTCAC                ^GT[GC]AC
Tth111I       357  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AfaI         1032  GTAC                 GT^AC
             1332  GTAC                
BalI          666  TGGCCA               TGG^CCA
             1078  TGGCCA              
BpmI          339  CTCCAG               CTGGAG(16/14)
              180  CTGGAG              
BseRI        1138  GAGGAG               GAGGAG(10/8)
             1256  GAGGAG              
BsgI         1342  CTGCAC               GTGCAG(16/14)
             1190  GTGCAG              
BsmFI         474  GTCCC                GGGAC(10/14)
              879  GTCCC               
BstXI         321  CCAAGCCCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              596  CCAAGCAACTGG        
BstYI         623  AGATCC               [GA]^GATC[CT]
              971  AGATCC              
EarI          946  GAAGAG               CTCTTC(1/4)
             1039  GAAGAG              
HincII        354  GTTGAC               GT[CT]^[GA]AC
             1238  GTCAAC              
HindII        354  GTTGAC               GT[CT]^[GA]AC
             1238  GTCAAC              
Ksp632I       946  GAAGAG               CTCTTC(1/4)
             1039  GAAGAG              
MflI          623  AGATCC               [GA]^GATC[CT]
              971  AGATCC              
MluNI         666  TGGCCA               TGG^CCA
             1078  TGGCCA              
MscI          666  TGGCCA               TGG^CCA
             1078  TGGCCA              
MslI          885  CATTGTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1183  CACTGCAGTG          
PflMI          33  CCAGGGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1347  CCACACTTTGG         
PstI          427  CTGCAG               CTGCA^G
             1185  CTGCAG              
RsaI         1032  GTAC                 GT^AC
             1332  GTAC                
SfaNI         459  GATGC                GCATC(5/9)
              977  GCATC               
SfcI          427  CTGCAG               C^T[GA][CT]AG
             1185  CTGCAG              
SmaI          515  CCCGGG               CCC^GGG
             1165  CCCGGG              
Tsp509I       614  AATT                 ^AATT
             1230  AATT                
Van91I         33  CCAGGGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1347  CCACACTTTGG         
XhoII         623  AGATCC               [GA]^GATC[CT]
              971  AGATCC              
XmaCI         515  CCCGGG               C^CCGGG
             1165  CCCGGG              
XmaI          515  CCCGGG               C^CCGGG
             1165  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AciI        AluI        AlwI        ApaI        AvaI        
AvaII       BanII       BbvI        BcnI        BfaI        
BmyI        BsaJI       BsiYI       BslI        BsoBI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HpaII       HphI        
ItaI        MaeI        MboI        MboII       MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PvuII       Sau3AI      
Sau96I      ScrFI       StyI        TseI        TspRI       
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