Restriction map for
pF1KB0405
(Circular) (Six-base) MAPSORT of: pF1KB0405.seq Check: 7266 from: 1 to: 4508
pF1KB0405 4508 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AccI GT'mk_AC
Cuts at: 914 1546 914
Size: 632 3876
Acc65I G'GTAC_C
Cuts at: 1524 1524
Size: 4508
AclI AA'CG_TT
Cuts at: 4348 4348
Size: 4508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 667 2333 2765 3512 3780 352
Size: 315 1666 432 747 268 1080
Fragments arranged by size:
1666 1080 747 432 315 268
AflIII A'CryG_T
Cuts at: 1860 2964 3941 1860
Size: 1104 977 2427
Fragments arranged by size:
2427 1104 977
AgeI A'CCGG_T
Cuts at: 2896 2896
Size: 4508
AhdI GACnn_n'nnGTC
Cuts at: 579 579
Size: 4508
AleI CACnn'nnGTG
Cuts at: 1295 1295
Size: 4508
AlwNI CAG_nnn'CTG
Cuts at: 1449 1813 3380 1449
Size: 364 1567 2577
Fragments arranged by size:
2577 1567 364
ApaI G_GGCC'C
Cuts at: 162 406 681 934 1220 162
Size: 244 275 253 286 3450
Fragments arranged by size:
3450 286 275 253 244
ApaLI G'TGCA_C
Cuts at: 3278 3278
Size: 4508
ApoI r'AATT_y
Cuts at: 721 1512 3861 4117 721
Size: 791 2349 256 1112
Fragments arranged by size:
2349 1112 791 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4508
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4508
AvaI C'yCGr_G
Cuts at: 623 1273 1361 1528 623
Size: 650 88 167 3603
Fragments arranged by size:
3603 650 167 88
BamHI G'GATC_C
Cuts at: 1533 1533
Size: 4508
BanI G'GyrC_C
Cuts at: 1524 1938 2186 2221 1524
Size: 414 248 35 3811
Fragments arranged by size:
3811 414 248 35
BanII G_rGCy'C
Cuts at: 162 406 553 681 934 1220 1255 1522 2552
Size: 244 147 128 253 286 35 267 1030
Cuts at: 2552 162
Size: 2118
Fragments arranged by size:
2118 1030 286 267 253 244 147 128
35
BbeI G_GCGC'C
Cuts at: 1942 2190 1942
Size: 248 4260
BbsI GAAGACnn'nnnn_
Cuts at: 764 764
Size: 4508
BbvCI CC'TCA_GC
Cuts at: 418 418
Size: 4508
BciVI GTATCCnnnnn_n'
Cuts at: 2406 3167 4508 2406
Size: 761 1341 2406
Fragments arranged by size:
2406 1341 761
BclI T'GATC_A
Cuts at: 2028 2028
Size: 4508
BglI GCCn_nnn'nGGC
Cuts at: 1316 1316
Size: 4508
BglII A'GATC_T
Cuts at: 2023 4060 2023
Size: 2037 2471
BlpI GC'TnA_GC
Cuts at: 1620 1620
Size: 4508
Bme1580I G_kGCm'C
Cuts at: 162 173 406 681 846 934 1108 1220 2133
Size: 11 233 275 165 88 174 112 913
Cuts at: 2133 2226 3282 162
Size: 93 1056 1388
Fragments arranged by size:
1388 1056 913 275 233 174 165 112
93 88 11
BmrI ACTGGGnnnn_n'
Cuts at: 1720 1801 2136 4280 1720
Size: 81 335 2144 1948
Fragments arranged by size:
2144 1948 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 410 2875 410
Size: 2465 2043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 432 309
Size: 123 4385
Bpu10I CC'TnA_GC
Cuts at: 418 418
Size: 4508
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1684 2563 3055 3353 1684
Size: 879 492 298 2839
Fragments arranged by size:
2839 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4212 4212
Size: 4508
BsaAI yAC'GTr
Cuts at: 1861 1861
Size: 4508
BsaBI GATnn'nnATC
Cuts at: 6 1084 2022 4059 6
Size: 1078 938 2037 455
Fragments arranged by size:
2037 1078 938 455
BsaHI Gr'CG_yC
Cuts at: 1939 2187 2889 4385 1939
Size: 248 702 1496 2062
Fragments arranged by size:
2062 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1697 2218 2896 3170 3317 3728 1697
Size: 521 678 274 147 411 2477
Fragments arranged by size:
2477 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 480 510 955 985 480
Size: 30 445 30 4003
Fragments arranged by size:
4003 445 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1261 1379 1261
Size: 118 4390
BseYI C'CCAG_C
Cuts at: 234 405 491 674 697 1213 1495 1933 2691
Size: 171 86 183 23 516 282 438 758
Cuts at: 2691 3268 3764 234
Size: 577 496 978
Fragments arranged by size:
978 758 577 516 496 438 282 183
171 86 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1319 1435 1319
Size: 116 4392
BsiEI CG_ry'CG
Cuts at: 104 2050 2096 3304 104
Size: 1946 46 1208 1308
Fragments arranged by size:
1946 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 456 1522 2300 2490 3282 456
Size: 1066 778 190 792 1682
Fragments arranged by size:
1682 1066 792 778 190
BsmI GAATG_Cn'
Cuts at: 233 233
Size: 4508
Bsp1286I G_dGCh'C
Cuts at: 162 173 406 456 553 681 846 934 1108
Size: 11 233 50 97 128 165 88 174
Cuts at: 1108 1220 1255 1522 2133 2226 2300 2490 2552
Size: 112 35 267 611 93 74 190 62
Cuts at: 2552 3282 162
Size: 730 1388
Fragments arranged by size:
1388 730 611 267 233 190 174 165
128 112 97 93 88 74 62 50
35 11
BspEI T'CCGG_A
Cuts at: 3728 3728
Size: 4508
BspHI T'CATG_A
Cuts at: 3684 3684
Size: 4508
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1558 1772 2074 2455 1558
Size: 214 302 381 3611
Fragments arranged by size:
3611 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2800 2854 4338 4503 50
Size: 2750 54 1484 165 55
Fragments arranged by size:
2750 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 917 2420 917
Size: 1503 3005
BsrFI r'CCGG_y
Cuts at: 1154 2506 2687 2896 1154
Size: 1352 181 209 2766
Fragments arranged by size:
2766 1352 209 181
BssSI C'ACGA_G
Cuts at: 2779 3137 2779
Size: 358 4150
BstAPI GCAn_nnn'nTGC
Cuts at: 1885 1885
Size: 4508
BstBI TT'CG_AA
Cuts at: 2869 2869
Size: 4508
BstEII G'GTnAC_C
Cuts at: 630 630
Size: 4508
BstXI CCAn_nnnn'nTGG
Cuts at: 436 711 436
Size: 275 4233
BstYI r'GATC_y
Cuts at: 1 731 1079 1533 2000 2023 2358 2604 3616
Size: 730 348 454 467 23 335 246 1012
Cuts at: 3616 3702 3714 4060 1
Size: 86 12 346 449
Fragments arranged by size:
1012 730 467 454 449 348 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1487 1487
Size: 4508
BtgI C'CryG_G
Cuts at: 107 435 107
Size: 328 4180
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1311 2458 2639 2802 1311
Size: 1147 181 163 3017
Fragments arranged by size:
3017 1147 181 163
BtsI GCAGTG_nn'
Cuts at: 650 1290 1302 1751 650
Size: 640 12 449 3407
Fragments arranged by size:
3407 640 449 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1320 1355 1320
Size: 35 4473
DraI TTT'AAA
Cuts at: 1507 1507
Size: 4508
DrdI GACnn_nn'nnGTC
Cuts at: 2214 3072 2214
Size: 858 3650
EaeI y'GGCC_r
Cuts at: 774 1186 1308 2093 2267 2658 2685 4030 774
Size: 412 122 785 174 391 27 1345 1252
Fragments arranged by size:
1345 1252 785 412 391 174 122 27
EagI C'GGCC_G
Cuts at: 2093 2093
Size: 4508
EarI CTCTTCn'nnn_
Cuts at: 1049 1142 2531 2741 1049
Size: 93 1389 210 2816
Fragments arranged by size:
2816 1389 210 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 281 488 2716 3026 3172 4318 281
Size: 207 2228 310 146 1146 471
Fragments arranged by size:
2228 1146 471 310 207 146
EcoICRI GAG'CTC
Cuts at: 1520 1520
Size: 4508
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 352 432 667 2333 2765 3512 3780 309
Size: 43 80 235 1666 432 747 268 1037
Fragments arranged by size:
1666 1037 747 432 268 235 80 43
EcoO109I rG'GnC_Cy
Cuts at: 151 159 678 930 931 1217 1647 151
Size: 8 519 252 1 286 430 3012
Fragments arranged by size:
3012 519 430 286 252 8 1
EcoRI G'AATT_C
Cuts at: 721 1512 721
Size: 791 3717
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 822 2527 822
Size: 1705 2803
FspI TGC'GCA
Cuts at: 649 2289 649
Size: 1640 2868
HaeII r_GCGC'y
Cuts at: 447 690 1155 1942 2190 3212 4191 447
Size: 243 465 787 248 1022 979 764
Fragments arranged by size:
1022 979 787 764 465 248 243
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 480 512 565 597 854 886 970 1002 1141
Size: 32 53 32 257 32 84 32 139
Cuts at: 1141 1173 2350 2380 2382 2412 480
Size: 32 1177 30 2 30 2576
Fragments arranged by size:
2576 1177 257 139 84 53 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 464 1348 1547 464
Size: 884 199 3425
Fragments arranged by size:
3425 884 199
KasI G'GCGC_C
Cuts at: 1938 2186 1938
Size: 248 4260
KpnI G_GTAC'C
Cuts at: 1528 1528
Size: 4508
MluI A'CGCG_T
Cuts at: 3941 3941
Size: 4508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 932 3179 3363 932
Size: 2247 184 2077
Fragments arranged by size:
2247 2077 184
MscI TGG'CCA
Cuts at: 776 1188 2269 776
Size: 412 1081 3015
Fragments arranged by size:
3015 1081 412
MslI CAynn'nnrTG
Cuts at: 997 1295 2497 2624 997
Size: 298 1202 127 2881
Fragments arranged by size:
2881 1202 298 127
MspA1I CmG'CkG
Cuts at: 204 234 335 363 674 1213 1353 1482 1619
Size: 30 101 28 311 539 140 129 137
Cuts at: 1619 1933 2293 3306 3551 204
Size: 314 360 1013 245 1161
Fragments arranged by size:
1161 1013 539 360 314 311 245 140
137 129 101 30 28
NaeI GCC'GGC
Cuts at: 2689 2689
Size: 4508
NarI GG'CG_CC
Cuts at: 1939 2187 1939
Size: 248 4260
NcoI C'CATG_G
Cuts at: 107 435 107
Size: 328 4180
NgoMIV G'CCGG_C
Cuts at: 2687 2687
Size: 4508
NspI r_CATG'y
Cuts at: 529 1561 2061 2968 529
Size: 1032 500 907 2069
Fragments arranged by size:
2069 1032 907 500
PasI CC'CwG_GG
Cuts at: 141 399 585 927 141
Size: 258 186 342 3722
Fragments arranged by size:
3722 342 258 186
PciI A'CATG_T
Cuts at: 2964 2964
Size: 4508
PflMI CCAn_nnn'nTGG
Cuts at: 147 1461 147
Size: 1314 3194
PfoI T'CCnGG_A
Cuts at: 286 1088 286
Size: 802 3706
PmeI GTTT'AAAC
Cuts at: 1507 1507
Size: 4508
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3647 3679 3647
Size: 32 4476
PspOMI G'GGCC_C
Cuts at: 158 402 677 930 1216 158
Size: 244 275 253 286 3450
Fragments arranged by size:
3450 286 275 253 244
PstI C_TGCA'G
Cuts at: 539 1297 1555 2240 539
Size: 758 258 685 2807
Fragments arranged by size:
2807 758 685 258
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4508
PvuII CAG'CTG
Cuts at: 234 335 363 1213 1353 1482 1933 2293 234
Size: 101 28 850 140 129 451 360 2449
Fragments arranged by size:
2449 850 451 360 140 129 101 28
RsrII CG'GwC_CG
Cuts at: 2703 2703
Size: 4508
SacI G_AGCT'C
Cuts at: 1522 1522
Size: 4508
SalI G'TCGA_C
Cuts at: 1545 1545
Size: 4508
SapI GCTCTTCn'nnn_
Cuts at: 1142 2531 2741 1142
Size: 1389 210 2909
Fragments arranged by size:
2909 1389 210
SbfI CC_TGCA'GG
Cuts at: 1555 1555
Size: 4508
ScaI AGT'ACT
Cuts at: 4025 4025
Size: 4508
SfcI C'TryA_G
Cuts at: 33 535 1293 1551 2236 3229 3420 33
Size: 502 758 258 685 993 191 1121
Fragments arranged by size:
1121 993 758 685 502 258 191
SfoI GGC'GCC
Cuts at: 1940 2188 1940
Size: 248 4260
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4508
SmaI CCC'GGG
Cuts at: 625 1275 1530 625
Size: 650 255 3603
Fragments arranged by size:
3603 650 255
SmlI C'TyrA_G
Cuts at: 1663 2578 3070 3332 1663
Size: 915 492 262 2839
Fragments arranged by size:
2839 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4508
SphI G_CATG'C
Cuts at: 1561 2061 1561
Size: 500 4008
StuI AGG'CCT
Cuts at: 121 121
Size: 4508
StyI C'CwwG_G
Cuts at: 107 154 435 785 1642 1996 107
Size: 47 281 350 857 354 2619
Fragments arranged by size:
2619 857 354 350 281 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 148 237 331 569 711 806 940 2091 2224
Size: 89 94 238 142 95 134 1151 133
Cuts at: 2224 2683 2892 148
Size: 459 209 1764
Fragments arranged by size:
1764 1151 459 238 209 142 134 133
95 94 89
TatI w'GTAC_w
Cuts at: 4023 4023
Size: 4508
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 955 987 4025 4057 955
Size: 32 3038 32 1406
Fragments arranged by size:
3038 1406 32 32
Tth111I GACn'n_nGTC
Cuts at: 468 2305 468
Size: 1837 2671
XbaI T'CTAG_A
Cuts at: 1539 1539
Size: 4508
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2003 2003
Size: 4508
XmaI C'CCGG_G
Cuts at: 623 1273 1528 623
Size: 650 255 3603
Fragments arranged by size:
3603 650 255
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmBI BsrGI
BssHII BstZ17I ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI