Restriction map for pF1KB0405
 (Linear) (Six-base) MAPSORT of: KIBB0405.seq  Check: 5050  from: 1  to: 1395



KIBB0405.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:13  ..

AccI GT'mk_AC

Cuts at:      0     806    1395 
   Size:        806     589
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     559    1395 
   Size:        244     315     836

  Fragments arranged by size: 

                 836     315     244
 
AhdI GACnn_n'nnGTC

Cuts at:      0     471    1395 
   Size:        471     924
 
AleI CACnn'nnGTG

Cuts at:      0    1187    1395 
   Size:       1187     208
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1341    1395 
   Size:       1341      54
 
ApaI G_GGCC'C

Cuts at:      0      54     298     573     826    1112    1395 
   Size:         54     244     275     253     286     283

  Fragments arranged by size: 

                 286     283     275     253     244      54
 
ApoI r'AATT_y

Cuts at:      0     613    1395 
   Size:        613     782
 
AvaI C'yCGr_G

Cuts at:      0     515    1165    1253    1395 
   Size:        515     650      88     142

  Fragments arranged by size: 

                 650     515     142      88
 
BanII G_rGCy'C

Cuts at:      0      54     298     445     573     826    1112    1147    1395 
   Size:         54     244     147     128     253     286      35     248

  Fragments arranged by size: 

                 286     253     248     244     147     128      54      35
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     656    1395 
   Size:        656     739
 
BbvCI CC'TCA_GC

Cuts at:      0     310    1395 
   Size:        310    1085
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1208    1395 
   Size:       1208     187
 
Bme1580I G_kGCm'C

Cuts at:      0      54      65     298     573     738     826    1000    1112 
   Size:         54      11     233     275     165      88     174     112

Cuts at:   1112    1395 
   Size:        283

  Fragments arranged by size: 

                 283     275     233     174     165     112      88      54
                  11
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     302    1395 
   Size:        302    1093
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     201     324    1395 
   Size:        201     123    1071

  Fragments arranged by size: 

                1071     201     123
 
Bpu10I CC'TnA_GC

Cuts at:      0     310    1395 
   Size:        310    1085
 
BsaBI GATnn'nnATC

Cuts at:      0     976    1395 
   Size:        976     419
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     372     402     847     877    1395 
   Size:        372      30     445      30     518

  Fragments arranged by size: 

                 518     445     372      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1153    1271    1395 
   Size:       1153     118     124

  Fragments arranged by size: 

                1153     124     118
 
BseYI C'CCAG_C

Cuts at:      0     126     297     383     566     589    1105    1387    1395 
   Size:        126     171      86     183      23     516     282       8

  Fragments arranged by size: 

                 516     282     183     171     126      86      23       8
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1211    1327    1395 
   Size:       1211     116      68

  Fragments arranged by size: 

                1211     116      68
 
BsiHKAI G_wGCw'C

Cuts at:      0     348    1395 
   Size:        348    1047
 
BsmI GAATG_Cn'

Cuts at:      0     125    1395 
   Size:        125    1270
 
Bsp1286I G_dGCh'C

Cuts at:      0      54      65     298     348     445     573     738     826 
   Size:         54      11     233      50      97     128     165      88

Cuts at:    826    1000    1112    1147    1395 
   Size:        174     112      35     248

  Fragments arranged by size: 

                 248     233     174     165     128     112      97      88
                  54      50      35      11
 
BsrDI GCAATG_nn'

Cuts at:      0     809    1395 
   Size:        809     586
 
BsrFI r'CCGG_y

Cuts at:      0    1046    1395 
   Size:       1046     349
 
BstEII G'GTnAC_C

Cuts at:      0     522    1395 
   Size:        522     873
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     328     603    1395 
   Size:        328     275     792

  Fragments arranged by size: 

                 792     328     275
 
BstYI r'GATC_y

Cuts at:      0     623     971    1395 
   Size:        623     348     424

  Fragments arranged by size: 

                 623     424     348
 
Bsu36I CC'TnA_GG

Cuts at:      0    1379    1395 
   Size:       1379      16
 
BtgI C'CryG_G

Cuts at:      0     327    1395 
   Size:        327    1068
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1203    1395 
   Size:       1203     192
 
BtsI GCAGTG_nn'

Cuts at:      0     542    1182    1194    1395 
   Size:        542     640      12     201

  Fragments arranged by size: 

                 640     542     201      12
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1212    1247    1395 
   Size:       1212      35     148

  Fragments arranged by size: 

                1212     148      35
 
EaeI y'GGCC_r

Cuts at:      0     666    1078    1200    1395 
   Size:        666     412     122     195

  Fragments arranged by size: 

                 666     412     195     122
 
EarI CTCTTCn'nnn_

Cuts at:      0     941    1034    1395 
   Size:        941      93     361

  Fragments arranged by size: 

                 941     361      93
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     173     380    1395 
   Size:        173     207    1015

  Fragments arranged by size: 

                1015     207     173
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     201     244     324     559    1395 
   Size:        201      43      80     235     836

  Fragments arranged by size: 

                 836     235     201      80      43
 
EcoO109I rG'GnC_Cy

Cuts at:      0      43      51     570     822     823    1109    1395 
   Size:         43       8     519     252       1     286     286

  Fragments arranged by size: 

                 519     286     286     252      43       8       1
 
EcoRI G'AATT_C

Cuts at:      0     613    1395 
   Size:        613     782
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     714    1395 
   Size:        714     681
 
FspI TGC'GCA

Cuts at:      0     541    1395 
   Size:        541     854
 
HaeII r_GCGC'y

Cuts at:      0     339     582    1047    1395 
   Size:        339     243     465     348

  Fragments arranged by size: 

                 465     348     339     243
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     372     404     457     489     746     778     862     894 
   Size:        372      32      53      32     257      32      84      32

Cuts at:    894    1033    1065    1395 
   Size:        139      32     330

  Fragments arranged by size: 

                 372     330     257     139      84      53      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     356    1240    1395 
   Size:        356     884     155

  Fragments arranged by size: 

                 884     356     155
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     824    1395 
   Size:        824     571
 
MscI TGG'CCA

Cuts at:      0     668    1080    1395 
   Size:        668     412     315

  Fragments arranged by size: 

                 668     412     315
 
MslI CAynn'nnrTG

Cuts at:      0     889    1187    1395 
   Size:        889     298     208

  Fragments arranged by size: 

                 889     298     208
 
MspA1I CmG'CkG

Cuts at:      0      96     126     227     255     566    1105    1245    1374 
   Size:         96      30     101      28     311     539     140     129

Cuts at:   1374    1395 
   Size:         21

  Fragments arranged by size: 

                 539     311     140     129     101      96      30      28
                  21
 
NcoI C'CATG_G

Cuts at:      0     327    1395 
   Size:        327    1068
 
NspI r_CATG'y

Cuts at:      0     421    1395 
   Size:        421     974
 
PasI CC'CwG_GG

Cuts at:      0      33     291     477     819    1395 
   Size:         33     258     186     342     576

  Fragments arranged by size: 

                 576     342     258     186      33
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      39    1353    1395 
   Size:         39    1314      42

  Fragments arranged by size: 

                1314      42      39
 
PfoI T'CCnGG_A

Cuts at:      0     178     980    1395 
   Size:        178     802     415

  Fragments arranged by size: 

                 802     415     178
 
PspOMI G'GGCC_C

Cuts at:      0      50     294     569     822    1108    1395 
   Size:         50     244     275     253     286     287

  Fragments arranged by size: 

                 287     286     275     253     244      50
 
PstI C_TGCA'G

Cuts at:      0     431    1189    1395 
   Size:        431     758     206

  Fragments arranged by size: 

                 758     431     206
 
PvuII CAG'CTG

Cuts at:      0     126     227     255    1105    1245    1374    1395 
   Size:        126     101      28     850     140     129      21

  Fragments arranged by size: 

                 850     140     129     126     101      28      21
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1034    1395 
   Size:       1034     361
 
SfcI C'TryA_G

Cuts at:      0     427    1185    1395 
   Size:        427     758     210

  Fragments arranged by size: 

                 758     427     210
 
SmaI CCC'GGG

Cuts at:      0     517    1167    1395 
   Size:        517     650     228

  Fragments arranged by size: 

                 650     517     228
 
StuI AGG'CCT

Cuts at:      0      13    1395 
   Size:         13    1382
 
StyI C'CwwG_G

Cuts at:      0      46     327     677    1395 
   Size:         46     281     350     718

  Fragments arranged by size: 

                 718     350     281      46
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      40     129     223     461     603     698     832    1395 
   Size:         40      89      94     238     142      95     134     563

  Fragments arranged by size: 

                 563     238     142     134      95      94      89      40
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     847     879    1395 
   Size:        847      32     516

  Fragments arranged by size: 

                 847     516      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     360    1395 
   Size:        360    1035
 
XmaI C'CCGG_G

Cuts at:      0     515    1165    1395 
   Size:        515     650     230

  Fragments arranged by size: 

                 650     515     230
 

 Enzymes that do cut:

     AccI     AcuI     AhdI     AleI    AlwNI     ApaI     ApoI     AvaI
    BanII     BbsI    BbvCI     BglI Bme1580I     BplI     BpmI   Bpu10I
    BsaBI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BsrDI    BsrFI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI    CspCI     EaeI     EarI     EciI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     MmeI     MscI     MslI
   MspA1I     NcoI     NspI     PasI    PflMI     PfoI   PspOMI     PstI
    PvuII     SapI     SfcI     SmaI     StuI     StyI    TaqII     TstI
  Tth111I     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI    BmgBI     BmrI     BmtI    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I     ClaI     DraI
   DraIII     DrdI     EagI  EcoICRI    EcoNI    EcoRV     FseI    FspAI
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     TatI     XbaI     XcmI     XhoI
     XmnI     ZraI
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