Restriction map for pF1KB6687
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AflII       AflIII      AgeI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspI        AvaII       AvrII       
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BcnI        BfrI        BglII       
BlnI        BpmI        BsaAI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmAI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
Cfr10I      ClaI        CpoI        DraII       DraIII      
DrdI        DsaI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
FseI        HaeII       HapII       HindIII     HpaI        
HpaII       HphI        KasI        KpnI        KspI        
MfeI        MluI        MroI        MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIV       NotI        
NruI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshBI       PvuI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
Tth111I     TthHB8I     Van91I      XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AciI          204  GCGG                 CCGC(-3/-1)
AclI          613  AACGTT               AA^CGTT
AcyI          546  GACGCC               G[GA]^CG[CT]C
AhdI          619  GACATTTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         619  GACATTTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          580  CCCGAG               C^[CT]CG[GA]G
AviII         121  TGCGCA               TGC^GCA
BalI          515  TGGCCA               TGG^CCA
BbsI         1197  GTCTTC               GAAGAC(2/6)
BlpI          168  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I      168  GCTAAGC              GC^T[ACGT]AGC
BpuAI        1197  GTCTTC               GAAGAC(2/6)
BsaBI         334  GATGCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         546  GACGCC               G[GA]^CG[CT]C
BseRI        1090  GAGGAG               GAGGAG(10/8)
BsgI         1139  GTGCAG               GTGCAG(16/14)
BsiYI         591  CCTTGGCATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          591  CCTTGGCATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         449  GGGAC                GGGAC(10/14)
BsoBI         580  CCCGAG               C^[CT]CG[GA]G
BstXI         896  CCAAAATGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         168  GCTAAGC              GC^T[ACGT]AGC
CfoI          122  GCGC                 GCG^C
DraI           84  TTTAAA               TTT^AAA
EaeI          515  TGGCCA               [CT]^GGCC[GA]
Eam1105I      619  GACATTTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI         739  GAATTC               G^AATTC
EcoRV          74  GATATC               GAT^ATC
EcoT14I       591  CCTTGG               C^C[AT][AT]GG
EcoT22I       914  ATGCAT               ATGCA^T
FauI          204  GCGGG                CCCGC(4/6)
FspI          121  TGCGCA               TGC^GCA
HgaI          546  GACGC                GACGC(5/10)
HhaI          122  GCGC                 GCG^C
Hin1I         546  GACGCC               G[GA]^CG[CT]C
HinP1I        122  GCGC                 G^CGC
MamI          334  GATGCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         515  TGGCCA               TGG^CCA
MscI          515  TGGCCA               TGG^CCA
MslI          315  CATAGTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI          914  ATGCAT               ATGCA^T
Psp1406I      613  AACGTT               AA^CGTT
PstI          524  CTGCAG               CTGCA^G
SmlI          107  CTCAAG               C^T[CT][GA]AG
StyI          591  CCTTGG               C^C[AT][AT]GG
XbaI         1245  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AfaI          680  GTAC                 GT^AC
              957  GTAC                
AlwNI         400  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1253  CAGCAGCTG           
BanII         104  GAGCTC               G[GA]GC[CT]^C
              325  GAGCTC              
BglI          199  GCCAAGCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              871  GCCAGTCAGGC         
BspHI         372  TCATGA               T^CATGA
              743  TCATGA              
BsrI          461  CCAGT                ACTGG(1/-1)
              872  CCAGT               
BssKI         518  CCAGG                ^CC[ACGT]GG
              676  CCAGG               
BstNI         518  CCAGG                CC^[AT]GG
              676  CCAGG               
Cac8I          11  GCAAGC               GC[ACGT]^[ACGT]GC
              204  GCGGGC              
Cfr13I        206  GGGCC                G^G[ACGT]CC
             1261  GGGCC               
EarI          820  CTCTTC               CTCTTC(1/4)
             1048  GAAGAG              
EcoRII        518  CCAGG                ^CC[AT]GG
              676  CCAGG               
HincII        616  GTTGAC               GT[CT]^[GA]AC
             1206  GTCAAC              
HindII        616  GTTGAC               GT[CT]^[GA]AC
             1206  GTCAAC              
Ksp632I       820  CTCTTC               CTCTTC(1/4)
             1048  GAAGAG              
MaeII         614  ACGT                 A^CGT
              776  ACGT                
MaeIII         17  GTGAC                ^GT[ACGT]AC
              559  GTGAC               
MspA1I        842  CAGCTG               C[AC]G^C[GT]G
             1256  CAGCTG              
MvaI          518  CCAGG                CC^[AT]GG
              676  CCAGG               
PshAI         505  GACTTCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1237  GACAGCAGTC          
PvuII         842  CAGCTG               CAG^CTG
             1256  CAGCTG              
RcaI          372  TCATGA               T^CATGA
              743  TCATGA              
RsaI          680  GTAC                 GT^AC
              957  GTAC                
SacI          104  GAGCTC               GAGCT^C
              325  GAGCTC              
Sau96I        206  GGGCC                G^G[ACGT]CC
             1261  GGGCC               
ScrFI         518  CCAGG                CC^[ACGT]GG
              676  CCAGG               
Tsp45I         17  GTGAC                ^GT[GC]AC
              559  GTGAC               
TspRI         697  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1145  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AcsI        AluI        AlwI        ApoI        Asp700I     
AspHI       BbvI        BclI        BfaI        BmyI        
BsaJI       BsiHKAI     Bsp1286I    BstF5I      BstYI       
DdeI        DpnII       FbaI        Fnu4HI      FokI        
HaeIII      HinfI       ItaI        MaeI        MboI        
MboII       MflI        MnlI        MseI        MwoI        
NdeII       NlaIII      PleI        Sau3AI      SfaNI       
SfcI        TfiI        Tru9I       TseI        Tsp509I     
XhoII       XmnI        
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