Restriction map for pF1KB6679
 (Linear) (Six-base) MAPSORT of: KIBB6679.seq  Check: 6818  from: 1  to: 1281



KIBB6679.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     505    1281 
   Size:        505     776
 
AflIII A'CryG_T

Cuts at:      0    1118    1281 
   Size:       1118     163
 
AlwNI CAG_nnn'CTG

Cuts at:      0     186    1281 
   Size:        186    1095
 
ApoI r'AATT_y

Cuts at:      0     417     803    1195    1281 
   Size:        417     386     392      86

  Fragments arranged by size: 

                 417     392     386      86
 
AvaI C'yCGr_G

Cuts at:      0      94    1281 
   Size:         94    1187
 
BanI G'GyrC_C

Cuts at:      0     356    1281 
   Size:        356     925
 
BanII G_rGCy'C

Cuts at:      0      95     124     269     903    1281 
   Size:         95      29     145     634     378

  Fragments arranged by size: 

                 634     378     145      95      29
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      37    1281 
   Size:         37    1244
 
BglI GCCn_nnn'nGGC

Cuts at:      0     861     971    1281 
   Size:        861     110     310

  Fragments arranged by size: 

                 861     310     110
 
BmgBI CAC'GTC

Cuts at:      0    1119    1281 
   Size:       1119     162
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     488     948    1281 
   Size:        488     460     333

  Fragments arranged by size: 

                 488     460     333
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     922    1281 
   Size:        922     359
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     109     253     485    1272    1281 
   Size:        103       6     144     232     787       9

  Fragments arranged by size: 

                 787     232     144     103       9       6
 
BsaWI w'CCGG_w

Cuts at:      0     124     302    1281 
   Size:        124     178     979

  Fragments arranged by size: 

                 979     178     124
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      60      90     647     677    1281 
   Size:         60      30     557      30     604

  Fragments arranged by size: 

                 604     557      60      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      81     513    1281 
   Size:         81     432     768

  Fragments arranged by size: 

                 768     432      81
 
BseYI C'CCAG_C

Cuts at:      0     231     370    1281 
   Size:        231     139     911

  Fragments arranged by size: 

                 911     231     139
 
BsiEI CG_ry'CG

Cuts at:      0    1115    1281 
   Size:       1115     166
 
BsmI GAATG_Cn'

Cuts at:      0     722    1281 
   Size:        722     559
 
Bsp1286I G_dGCh'C

Cuts at:      0      95     124     269     903    1281 
   Size:         95      29     145     634     378

  Fragments arranged by size: 

                 634     378     145      95      29
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     821    1281 
   Size:        821     460
 
BsrDI GCAATG_nn'

Cuts at:      0    1235    1281 
   Size:       1235      46
 
BstEII G'GTnAC_C

Cuts at:      0    1234    1281 
   Size:       1234      47
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     378    1281 
   Size:        378     903
 
BstYI r'GATC_y

Cuts at:      0     182     932    1071    1281 
   Size:        182     750     139     210

  Fragments arranged by size: 

                 750     210     182     139
 
BtgI C'CryG_G

Cuts at:      0     377     914    1205    1281 
   Size:        377     537     291      76

  Fragments arranged by size: 

                 537     377     291      76
 
DraI TTT'AAA

Cuts at:      0     558    1281 
   Size:        558     723
 
EaeI y'GGCC_r

Cuts at:      0     247     773    1281 
   Size:        247     526     508

  Fragments arranged by size: 

                 526     508     247
 
EarI CTCTTCn'nnn_

Cuts at:      0     919    1281 
   Size:        919     362
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     109     253     485     505    1272    1281 
   Size:        103       6     144     232      20     767       9

  Fragments arranged by size: 

                 767     232     144     103      20       9       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     330     817    1281 
   Size:        330     487     464

  Fragments arranged by size: 

                 487     464     330
 
EcoO109I rG'GnC_Cy

Cuts at:      0     212     810    1281 
   Size:        212     598     471

  Fragments arranged by size: 

                 598     471     212
 
EcoRI G'AATT_C

Cuts at:      0    1195    1281 
   Size:       1195      86
 
EcoRV GAT'ATC

Cuts at:      0     405    1281 
   Size:        405     876
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      60      92     458     490    1117    1134    1149    1153 
   Size:         60      32     366      32     627      17      15       4

Cuts at:   1153    1166    1185    1281 
   Size:         13      19      96

  Fragments arranged by size: 

                 627     366      96      60      32      32      19      17
                  15      13       4
 
HincII GTy'rAC

Cuts at:      0     873    1281 
   Size:        873     408
 
MscI TGG'CCA

Cuts at:      0     249     775    1281 
   Size:        249     526     506

  Fragments arranged by size: 

                 526     506     249
 
MslI CAynn'nnrTG

Cuts at:      0    1054    1281 
   Size:       1054     227
 
NcoI C'CATG_G

Cuts at:      0    1205    1281 
   Size:       1205      76
 
NspI r_CATG'y

Cuts at:      0     742    1281 
   Size:        742     539
 
PasI CC'CwG_GG

Cuts at:      0     969    1281 
   Size:        969     312
 
PpuMI rG'GwC_Cy

Cuts at:      0     212     810    1281 
   Size:        212     598     471

  Fragments arranged by size: 

                 598     471     212
 
PvuI CG_AT'CG

Cuts at:      0    1115    1281 
   Size:       1115     166
 
SapI GCTCTTCn'nnn_

Cuts at:      0     919    1281 
   Size:        919     362
 
StyI C'CwwG_G

Cuts at:      0     347    1205    1281 
   Size:        347     858      76

  Fragments arranged by size: 

                 858     347      76
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     150     374    1029    1281 
   Size:        150     224     655     252

  Fragments arranged by size: 

                 655     252     224     150
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     647     679    1281 
   Size:        647      32     602

  Fragments arranged by size: 

                 647     602      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     761    1281 
   Size:        761     520
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApoI     AvaI     BanI    BanII     BbsI
     BglI    BmgBI     BmrI     BplI     BpmI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI     BsmI Bsp1286I    BspMI    BsrDI   BstEII    BstXI
    BstYI     BtgI     DraI     EaeI     EarI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV    Hin4I   HincII     MscI     MslI     NcoI     NspI
     PasI    PpuMI     PvuI     SapI     StyI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI Bme1580I     BmtI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI     BsgI  BsiHKAI    BsiWI    BsmBI
    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI   DraIII
     DrdI     EagI     EciI  EcoICRI     FalI     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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