Restriction map for pF1KB6677
##################################
# NO CUT ENZYMES (  143 enzymes) #
##################################

AatII       AccI        AccIII      AciI        AclI        
AcyI        AflIII      AgeI        AhdI        AlwNI       
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AvaI        AviII       
AvrII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BcnI        BfaI        BglI        
BglII       BlnI        BpuAI       BsaAI       BsaBI       
BsaHI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstZ17I     Cfr10I      ClaI        CpoI        
DraII       DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO109I    EcoT22I     FauI        FseI        FspI        
Hin1I       HindIII     HpaI        KasI        KspI        
MaeI        MaeII       MamI        MfeI        MluI        
MroI        MspA1I      MunI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tsp45I      
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

Acc65I        678  GGTACC               G^GTACC
AccII         348  CGCG                 CG^CG
AflII         892  CTTAAG               C^TTAAG
Alw44I        807  GTGCAC               G^TGCAC
AocI           15  CCTCAGG              CC^T[ACGT]AGG
ApaLI         807  GTGCAC               G^TGCAC
Asp718I       678  GGTACC               G^GTACC
BalI          647  TGGCCA               TGG^CCA
BamHI         629  GGATCC               G^GATCC
BanI          678  GGTACC               G^G[CT][GA]CC
BanII         130  GAGCTC               G[GA]GC[CT]^C
BclI          444  TGATCA               T^GATCA
BfrI          892  CTTAAG               C^TTAAG
BlpI          132  GCTCAGC              GC^T[ACGT]AGC
BpmI          567  CTGGAG               CTGGAG(16/14)
Bpu1102I      132  GCTCAGC              GC^T[ACGT]AGC
BsaI          374  GAGACC               GGTCTC(1/5)
BsaWI        1253  ACCGGA               [AT]^CCGG[AT]
BsmFI        1192  GTCCC                GGGAC(10/14)
BsmI          291  GAATGC               GAATGC(1/-1)
BspHI         830  TCATGA               T^CATGA
BsrDI         182  GCAATG               GCAATG(2/0)
BstEII        144  GGTTACC              G^GT[ACGT]ACC
BstPI         144  GGTTACC              G^GT[ACGT]ACC
BstUI         348  CGCG                 CG^CG
BstXI         161  CCACTAATTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         629  GGATCC               [GA]^GATC[CT]
Bsu36I         15  CCTCAGG              CC^T[ACGT]AGG
CelII         132  GCTCAGC              GC^T[ACGT]AGC
DraI         1126  TTTAAA               TTT^AAA
EaeI          647  TGGCCA               [CT]^GGCC[GA]
Eco81I         15  CCTCAGG              CC^T[ACGT]AGG
EcoO65I       144  GGTTACC              G^GT[ACGT]ACC
EcoRI        1080  GAATTC               G^AATTC
EcoRV         255  GATATC               GAT^ATC
FbaI          444  TGATCA               T^GATCA
HaeII         431  GGCGCT               [GA]GCGC^[CT]
HapII        1254  CCGG                 C^CGG
HgaI          900  GCGTC                GACGC(5/10)
HincII        541  GTTGAC               GT[CT]^[GA]AC
HindII        541  GTTGAC               GT[CT]^[GA]AC
HpaII        1254  CCGG                 C^CGG
KpnI          678  GGTACC               GGTAC^C
MflI          629  GGATCC               [GA]^GATC[CT]
MluNI         647  TGGCCA               TGG^CCA
MscI          647  TGGCCA               TGG^CCA
MspI         1254  CCGG                 C^CGG
MvnI          348  CGCG                 CG^CG
PleI          570  GAGTC                GAGTC(4/5)
PshAI        1185  GACCTCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          830  TCATGA               T^CATGA
SacI          130  GAGCTC               GAGCT^C
SapI          774  GCTCTTC              GCTCTTC(1/4)
TaqI          903  TCGA                 T^CGA
TfiI          591  GATTC                G^A[AT]TC
TthHB8I       903  TCGA                 T^CGA
XhoII         629  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AspHI         130  GAGCTC               G[AT]GC[AT]^C
              807  GTGCAC              
AvaII         171  GGTCC                G^G[AT]CC
             1191  GGTCC               
BmyI          130  GAGCTC               G[AGT]GC[ACT]^C
              807  GTGCAC              
BsiHKAI       130  GAGCTC               G[AT]GC[AT]^C
              807  GTGCAC              
BsmAI         374  GAGAC                GTCTC(1/5)
              619  GTCTC               
Bsp1286I      130  GAGCTC               G[AGT]GC[ACT]^C
              807  GTGCAC              
EarI          775  CTCTTC               CTCTTC(1/4)
              962  CTCTTC              
EcoT14I       369  CCTTGG               C^C[AT][AT]GG
              800  CCTTGG              
HinfI         570  GAGTC                G^A[ACGT]TC
              591  GATTC               
Ksp632I       775  CTCTTC               CTCTTC(1/4)
              962  CTCTTC              
MslI           97  CATGTGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              825  CACCCTCATG          
SfaNI          22  GCATC                GCATC(5/9)
             1211  GCATC               
SmlI          867  CTTGAG               C^T[CT][GA]AG
              892  CTTAAG              
StyI          369  CCTTGG               C^C[AT][AT]GG
              800  CCTTGG              


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
BbvI        BsaJI       BsiYI       BslI        BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EcoRII      Fnu4HI      
FokI        HaeIII      HhaI        HinP1I      HphI        
ItaI        MaeIII      MboI        MboII       MnlI        
MseI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
Tru9I       TseI        Tsp509I     TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com