Restriction map for pF1KB6653
##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AflII       AflIII      AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BcgI        BfrI        
BglI        BglII       BlnI        BpmI        BsaAI       
BsaBI       BsaHI       BseRI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        BspDI       BspHI       
BspLU11I    BsrDI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HaeII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MflI        
MluI        MluNI       MscI        MseI        MspA1I      
MunI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PflFI       PflMI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        ScaI        
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SspI        SwaI        Tru9I       
Tth111I     Van91I      XbaI        XcmI        XhoII       
XmnI        


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# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI          427  GTAGAC               GT^[AC][GT]AC
AccIII       1086  TCCGGA               T^CCGGA
AcsI         1123  GAATTT               [GA]^AATT[CT]
AgeI          584  ACCGGT               A^CCGGT
AlwI          504  GATCC                GGATC(4/5)
ApoI         1123  GAATTT               [GA]^AATT[CT]
BbvCI         315  GCTGAGG              CCTCAGC(-5/-2)
BciVI         405  GGATAC               GTATCC(6/5)
BclI         1215  TGATCA               T^GATCA
BfaI          463  CTAG                 C^TAG
BlpI          834  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      834  GCTCAGC              GC^T[ACGT]AGC
BsaI          859  GGTCTC               GGTCTC(1/5)
BseAI        1086  TCCGGA               T^CCGGA
Bsp1407I      815  TGTACA               T^GTACA
BspEI        1086  TCCGGA               T^CCGGA
BspMI         175  ACCTGC               ACCTGC(4/8)
BsrBI        1082  CCGCTC               CCGCTC(-3/-3)
BsrGI         815  TGTACA               T^GTACA
BssSI         886  CACGAG               CACGAG(-5/-1)
CelII         834  GCTCAGC              GC^T[ACGT]AGC
CfoI          179  GCGC                 GCG^C
DsaI          630  CCATGG               C^C[GA][CT]GG
EaeI          192  TGGCCG               [CT]^GGCC[GA]
EcoNI         609  CCTGGACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       630  CCATGG               C^C[AT][AT]GG
FbaI         1215  TGATCA               T^GATCA
HhaI          179  GCGC                 GCG^C
HinP1I        179  GCGC                 G^CGC
MaeI          463  CTAG                 C^TAG
MaeII         659  ACGT                 A^CGT
MroI         1086  TCCGGA               T^CCGGA
MslI          912  CACCATCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI          630  CCATGG               C^CATGG
NspI          333  GCATGC               [GA]CATG^[CT]
PinAI         584  ACCGGT               A^CCGGT
SbfI          300  CCTGCAGG             CCTGCA^GG
SexAI         782  ACCAGGT              A^CC[AT]GGT
SmaI           13  CCCGGG               CCC^GGG
SphI          333  GCATGC               GCATG^C
Sse8387I      300  CCTGCAGG             CCTGCA^GG
SspBI         815  TGTACA               T^GTACA
StyI          630  CCATGG               C^C[AT][AT]GG
Tsp509I      1124  AATT                 ^AATT
XmaCI          13  CCCGGG               C^CCGGG
XmaI           13  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

BanI          270  GGTGCC               G^G[CT][GA]CC
              928  GGTGCC              
BanII          39  GAGCCC               G[GA]GC[CT]^C
              637  GAGCCC              
BbsI          199  GAAGAC               GAAGAC(2/6)
               25  GTCTTC              
BpuAI         199  GAAGAC               GAAGAC(2/6)
               25  GTCTTC              
BsaWI         584  ACCGGT               [AT]^CCGG[AT]
             1086  TCCGGA              
BsgI          592  CTGCAC               GTGCAG(16/14)
              514  GTGCAG              
BsrFI         308  ACCGGC               [GA]^CCGG[CT]
              584  ACCGGT              
BsrI          744  CCAGT                ACTGG(1/-1)
             1074  CCAGT               
Cfr10I        308  ACCGGC               [GA]^CCGG[CT]
              584  ACCGGT              
DdeI          316  CTGAG                C^T[ACGT]AG
              835  CTCAG               
EarI          730  CTCTTC               CTCTTC(1/4)
              466  GAAGAG              
Ksp632I       730  CTCTTC               CTCTTC(1/4)
              466  GAAGAG              
PaeR7I        598  CTCGAG               C^TCGAG
              992  CTCGAG              
PstI          301  CTGCAG               CTGCA^G
              388  CTGCAG              
StuI          257  AGGCCT               AGG^CCT
              416  AGGCCT              
TspRI         386  CACTG                CA[GC]TG[ACGT][ACGT]^
              830  CACTG               
XhoI          598  CTCGAG               C^TCGAG
              992  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AciI        AfaI        AluI        AvaI        AvaII       
BbvI        BcnI        BmyI        BsaJI       BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DpnII       EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        MaeIII      MboI        MboII       MnlI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PshAI       RsaI        Sau3AI      
Sau96I      ScrFI       SfcI        SmlI        TaqI        
TfiI        TseI        Tsp45I      TthHB8I     
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