Restriction map for pF1KB6653
 (Linear) (Six-base) MAPSORT of: KIBB6653.seq  Check: 846  from: 1  to: 1227



KIBB6653.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     184    1227 
   Size:        184    1043
 
AccI GT'mk_AC

Cuts at:      0     428    1227 
   Size:        428     799
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     533     717    1207    1227 
   Size:        166     367     184     490      20

  Fragments arranged by size: 

                 490     367     184     166      20
 
AgeI A'CCGG_T

Cuts at:      0     584    1227 
   Size:        584     643
 
AleI CACnn'nnGTG

Cuts at:      0     916    1227 
   Size:        916     311
 
ApoI r'AATT_y

Cuts at:      0    1123    1227 
   Size:       1123     104
 
AvaI C'yCGr_G

Cuts at:      0      13     598     992    1227 
   Size:         13     585     394     235

  Fragments arranged by size: 

                 585     394     235      13
 
BanI G'GyrC_C

Cuts at:      0     270     928    1227 
   Size:        270     658     299

  Fragments arranged by size: 

                 658     299     270
 
BanII G_rGCy'C

Cuts at:      0      43     641    1227 
   Size:         43     598     586

  Fragments arranged by size: 

                 598     586      43
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      18     206    1227 
   Size:         18     188    1021

  Fragments arranged by size: 

                1021     188      18
 
BbvCI CC'TCA_GC

Cuts at:      0     316    1227 
   Size:        316     911
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     399    1227 
   Size:        399     828
 
BclI T'GATC_A

Cuts at:      0    1215    1227 
   Size:       1215      12
 
BlpI GC'TnA_GC

Cuts at:      0     835    1227 
   Size:        835     392
 
Bme1580I G_kGCm'C

Cuts at:      0     275    1227 
   Size:        275     952
 
BmgBI CAC'GTC

Cuts at:      0     660    1227 
   Size:        660     567
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     738    1068    1227 
   Size:        738     330     159

  Fragments arranged by size: 

                 738     330     159
 
Bpu10I CC'TnA_GC

Cuts at:      0     316    1227 
   Size:        316     911
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     865    1227 
   Size:        865     362
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     865    1227 
   Size:        865     362
 
BsaWI w'CCGG_w

Cuts at:      0     584    1086    1227 
   Size:        584     502     141

  Fragments arranged by size: 

                 584     502     141
 
BseYI C'CCAG_C

Cuts at:      0     213     324     542     793     945    1227 
   Size:        213     111     218     251     152     282

  Fragments arranged by size: 

                 282     251     218     213     152     111
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     535     577    1227 
   Size:        535      42     650

  Fragments arranged by size: 

                 650     535      42
 
Bsp1286I G_dGCh'C

Cuts at:      0      43     275     641    1227 
   Size:         43     232     366     586

  Fragments arranged by size: 

                 586     366     232      43
 
BspEI T'CCGG_A

Cuts at:      0    1086    1227 
   Size:       1086     141
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     184    1227 
   Size:        184    1043
 
BsrBI CCG'CTC

Cuts at:      0    1084    1227 
   Size:       1084     143
 
BsrFI r'CCGG_y

Cuts at:      0     308     584    1227 
   Size:        308     276     643

  Fragments arranged by size: 

                 643     308     276
 
BsrGI T'GTAC_A

Cuts at:      0     815    1227 
   Size:        815     412
 
BssSI C'ACGA_G

Cuts at:      0     886    1227 
   Size:        886     341
 
BtgI C'CryG_G

Cuts at:      0     630    1227 
   Size:        630     597
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     228    1227 
   Size:        228     999
 
BtsI GCAGTG_nn'

Cuts at:      0     385     829    1227 
   Size:        385     444     398

  Fragments arranged by size: 

                 444     398     385
 
EaeI y'GGCC_r

Cuts at:      0     192    1227 
   Size:        192    1035
 
EarI CTCTTCn'nnn_

Cuts at:      0     461     736    1227 
   Size:        461     275     491

  Fragments arranged by size: 

                 491     461     275
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     533     717    1207    1227 
   Size:        166     367     184     490      20

  Fragments arranged by size: 

                 490     367     184     166      20
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     613    1227 
   Size:        613     614
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      96    1227 
   Size:         96    1131
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     772     804    1227 
   Size:        772      32     423

  Fragments arranged by size: 

                 772     423      32
 
MslI CAynn'nnrTG

Cuts at:      0     916    1227 
   Size:        916     311
 
NcoI C'CATG_G

Cuts at:      0     630    1227 
   Size:        630     597
 
NspI r_CATG'y

Cuts at:      0     337    1227 
   Size:        337     890
 
PshAI GACnn'nnGTC

Cuts at:      0      22    1074    1096    1227 
   Size:         22    1052      22     131

  Fragments arranged by size: 

                1052     131      22      22
 
PspXI vC'TCGA_Gb

Cuts at:      0     598    1227 
   Size:        598     629
 
PstI C_TGCA'G

Cuts at:      0     305     392    1227 
   Size:        305      87     835

  Fragments arranged by size: 

                 835     305      87
 
SbfI CC_TGCA'GG

Cuts at:      0     305    1227 
   Size:        305     922
 
SexAI A'CCwGG_T

Cuts at:      0     782    1227 
   Size:        782     445
 
SfcI C'TryA_G

Cuts at:      0     110     301     388     963    1227 
   Size:        110     191      87     575     264

  Fragments arranged by size: 

                 575     264     191     110      87
 
SmaI CCC'GGG

Cuts at:      0      15    1227 
   Size:         15    1212
 
SmlI C'TyrA_G

Cuts at:      0     598     880     992    1227 
   Size:        598     282     112     235

  Fragments arranged by size: 

                 598     282     235     112
 
SphI G_CATG'C

Cuts at:      0     337    1227 
   Size:        337     890
 
StuI AGG'CCT

Cuts at:      0     259     418    1227 
   Size:        259     159     809

  Fragments arranged by size: 

                 809     259     159
 
StyI C'CwwG_G

Cuts at:      0     630    1227 
   Size:        630     597
 
TatI w'GTAC_w

Cuts at:      0      62     623     746     815    1227 
   Size:         62     561     123      69     412

  Fragments arranged by size: 

                 561     412     123      69      62
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     554     586     941     973    1227 
   Size:        554      32     355      32     254

  Fragments arranged by size: 

                 554     355     254      32      32
 
XhoI C'TCGA_G

Cuts at:      0     598     992    1227 
   Size:        598     394     235

  Fragments arranged by size: 

                 598     394     235
 
XmaI C'CCGG_G

Cuts at:      0      13    1227 
   Size:         13    1214
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AgeI     AleI     ApoI     AvaI     BanI
    BanII     BbsI    BbvCI    BciVI     BclI     BlpI Bme1580I    BmgBI
     BmrI   Bpu10I    BpuEI     BsaI    BsaWI    BseYI     BsgI Bsp1286I
    BspEI    BspMI    BsrBI    BsrFI    BsrGI    BssSI     BtgI    BtgZI
     BtsI     EaeI     EarI  Eco57MI    EcoNI     FalI    Hin4I     MslI
     NcoI     NspI    PshAI    PspXI     PstI     SbfI    SexAI     SfcI
     SmaI     SmlI     SphI     StuI     StyI     TatI     TstI     XhoI
     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AhdI     AlfI
     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI     BcgI    BfrBI     BglI    BglII     BmtI
     BplI     BpmI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspHI    BsrDI   BssHII   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
     PsiI   PspOMI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     ScaI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     SspI     SwaI    TaqII  Tth111I     XbaI
     XcmI     XmnI     ZraI
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