Restriction map for pF1KB6630
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        Asp718I     AspEI       AspI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsiEI       BsiWI       BsmBI       BspDI       BspHI       
BspLU11I    BsrBI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraII       
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        SspI        SwaI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccII          63  CGCG                 CG^CG
AcyI          586  GACGCC               G[GA]^CG[CT]C
AseI          922  ATTAAT               AT^TAAT
AsnI          922  ATTAAT               AT^TAAT
Asp700I       296  GAAATGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         592  GTGCTC               G[AT]GC[AT]^C
AvaI          267  CCCGAG               C^[CT]CG[GA]G
BanII          77  GAGCCC               G[GA]GC[CT]^C
BbsI         1069  GTCTTC               GAAGAC(2/6)
BcnI          241  CCGGG                CC^[GC]GG
BfaI         1120  CTAG                 C^TAG
BglI          628  GCCCACAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI        1069  GTCTTC               GAAGAC(2/6)
BsaHI         586  GACGCC               G[GA]^CG[CT]C
BsaI          164  GAGACC               GGTCTC(1/5)
BsiHKAI       592  GTGCTC               G[AT]GC[AT]^C
BsmAI         164  GAGAC                GTCTC(1/5)
BsoBI         267  CCCGAG               C^[CT]CG[GA]G
Bsp1407I      434  TGTACA               T^GTACA
BsrDI        1212  GCAATG               GCAATG(2/0)
BsrFI          42  GCCGGT               [GA]^CCGG[CT]
BsrGI         434  TGTACA               T^GTACA
BstAPI        180  GCAGCCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          63  CGCG                 CG^CG
BstXI        1274  CCAGCCACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1100  AGATCC               [GA]^GATC[CT]
Cfr10I         42  GCCGGT               [GA]^CCGG[CT]
DraIII       1240  CACCCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI         344  CCTTCCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI          637  GCGGG                CCCGC(4/6)
HaeII        1013  AGCGCC               [GA]GCGC^[CT]
Hin1I         586  GACGCC               G[GA]^CG[CT]C
MaeI         1120  CTAG                 C^TAG
MaeII          98  ACGT                 A^CGT
MflI         1100  AGATCC               [GA]^GATC[CT]
MvnI           63  CGCG                 CG^CG
NciI          241  CCGGG                CC^[GC]GG
NspI          533  ACATGC               [GA]CATG^[CT]
PleI          385  GACTC                GAGTC(4/5)
PshBI         922  ATTAAT               AT^TAAT
ScaI          668  AGTACT               AGT^ACT
SspBI         434  TGTACA               T^GTACA
TaqI           70  TCGA                 T^CGA
Tsp509I       925  AATT                 ^AATT
TthHB8I        70  TCGA                 T^CGA
XhoII        1100  AGATCC               [GA]^GATC[CT]
XmnI          296  GAAATGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccIII        278  TCCGGA               T^CCGGA
              500  TCCGGA              
AlwNI         130  CAGTGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1138  CAGGGACTG           
BalI          680  TGGCCA               TGG^CCA
             1019  TGGCCA              
BanI         1217  GGCACC               G^G[CT][GA]CC
             1297  GGCACC              
BsaWI         278  TCCGGA               [AT]^CCGG[AT]
              500  TCCGGA              
BseAI         278  TCCGGA               T^CCGGA
              500  TCCGGA              
BsmI          262  GAATGC               GAATGC(1/-1)
              778  GCATTC              
BspEI         278  TCCGGA               T^CCGGA
              500  TCCGGA              
BspMI         413  ACCTGC               ACCTGC(4/8)
             1300  ACCTGC              
DsaI          440  CCGTGG               C^C[GA][CT]GG
             1244  CCGTGG              
HphI          287  TCACC                GGTGA(8/7)
              614  TCACC               
MaeIII         95  GTGAC                ^GT[ACGT]AC
              613  GTCAC               
MluNI         680  TGGCCA               TGG^CCA
             1019  TGGCCA              
MroI          278  TCCGGA               T^CCGGA
              500  TCCGGA              
MscI          680  TGGCCA               TGG^CCA
             1019  TGGCCA              
MseI          374  TTAA                 T^TAA
              923  TTAA                
PflMI         878  CCATCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1137  CCAGGGACTGG         
PvuII        1105  CAGCTG               CAG^CTG
             1186  CAGCTG              
SapI          118  GAAGAGC              GCTCTTC(1/4)
              594  GCTCTTC             
SbfI          135  CCTGCAGG             CCTGCA^GG
             1301  CCTGCAGG            
Sse8387I      135  CCTGCAGG             CCTGCA^GG
             1301  CCTGCAGG            
StuI          352  AGGCCT               AGG^CCT
              708  AGGCCT              
TfiI          272  GATTC                G^A[AT]TC
              953  GAATC               
Tru9I         374  TTAA                 T^TAA
              923  TTAA                
Tsp45I         95  GTGAC                ^GT[GC]AC
              613  GTCAC               
Van91I        878  CCATCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1137  CCAGGGACTGG         
XcmI          350  CCAGGCCTAATCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              827  CCAGACACTTTCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BbvI        BmyI        BpmI        BsaJI       BseRI       
BsgI        BsiYI       BslI        BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       EaeI        
EarI        EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       ItaI        Ksp632I     MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TseI        TspRI       
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