Restriction map for pF1KB6625
##################################
# NO CUT ENZYMES (  135 enzymes) #
##################################

Acc65I      AccI        AccIII      AcsI        AflII       
AflIII      AgeI        AhdI        AlwNI       AocI        
Aor51HI     ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvrII       
BbeI        BbrPI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BsaWI       BseAI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraIII      Eam1105I    EarI        Eco47III    
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HincII      HindII      
HindIII     HpaI        KasI        KpnI        Ksp632I     
MaeI        MfeI        MluI        MroI        MslI        
MunI        NarI        NciI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       PstI        PvuI        RcaI        RsrII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
Tsp509I     Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AatII         865  GACGTC               GACGT^C
AclI           58  AACGTT               AA^CGTT
Alw44I        436  GTGCAC               G^TGCAC
ApaLI         436  GTGCAC               G^TGCAC
AviII         762  TGCGCA               TGC^GCA
BamHI         697  GGATCC               G^GATCC
BanI         1156  GGCACC               G^G[CT][GA]CC
BbsI          493  GAAGAC               GAAGAC(2/6)
BlpI         1003  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1003  GCTGAGC              GC^T[ACGT]AGC
BpuAI         493  GAAGAC               GAAGAC(2/6)
BsaAI         547  TACGTG               [CT]AC^GT[GA]
BsaBI         274  GATTACGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI           43  GTGCAG               GTGCAG(16/14)
BsiEI        1178  CGGCCG               CG[GA][CT]^CG
BsmI          237  GAATGC               GAATGC(1/-1)
BspMI         387  ACCTGC               ACCTGC(4/8)
CelII        1003  GCTGAGC              GC^T[ACGT]AGC
EagI         1178  CGGCCG               C^GGCCG
EclXI        1178  CGGCCG               C^GGCCG
Eco52I       1178  CGGCCG               C^GGCCG
FspI          762  TGCGCA               TGC^GCA
HaeII         682  GGCGCT               [GA]GCGC^[CT]
MamI          274  GATTACGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI           29  TTAA                 T^TAA
NaeI         1176  GCCGGC               GCC^GGC
NcoI          431  CCATGG               C^CATGG
NgoMIV       1176  GCCGGC               G^CCGGC
Psp1406I       58  AACGTT               AA^CGTT
SacI         1077  GAGCTC               GAGCT^C
SfaNI         113  GCATC                GCATC(5/9)
TfiI          540  GAATC                G^A[AT]TC
Tru9I          29  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AfaI          306  GTAC                 GT^AC
              621  GTAC                
ApaI          530  GGGCCC               GGGCC^C
              897  GGGCCC              
BalI            2  TGGCCA               TGG^CCA
              450  TGGCCA              
BbvCI         165  CCTCAGC              CCTCAGC(-5/-2)
              409  CCTCAGC             
BsrFI         656  GCCGGT               [GA]^CCGG[CT]
             1176  GCCGGC              
BstF5I         48  GGATG                GGATG(2/0)
              856  GGATG               
BstYI         119  GGATCT               [GA]^GATC[CT]
              697  GGATCC              
Cfr10I        656  GCCGGT               [GA]^CCGG[CT]
             1176  GCCGGC              
DrdI          420  GACGCTGCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1017  GACCCGCAAGTC        
FokI           48  GGATG                GGATG(9/13)
              856  GGATG               
HapII         657  CCGG                 C^CGG
             1177  CCGG                
HpaII         657  CCGG                 C^CGG
             1177  CCGG                
KspI          147  CCGCGG               CCGC^GG
              320  CCGCGG              
MflI          119  GGATCT               [GA]^GATC[CT]
              697  GGATCC              
MluNI           2  TGGCCA               TGG^CCA
              450  TGGCCA              
MscI            2  TGGCCA               TGG^CCA
              450  TGGCCA              
MspI          657  CCGG                 C^CGG
             1177  CCGG                
NlaIII        432  CATG                 CATG^
              609  CATG                
PvuII        1041  CAGCTG               CAG^CTG
             1086  CAGCTG              
RsaI          306  GTAC                 GT^AC
              621  GTAC                
SacII         147  CCGCGG               CCGC^GG
              320  CCGCGG              
SmlI          214  CTTGAG               C^T[CT][GA]AG
              754  CTCAAG              
XhoII         119  GGATCT               [GA]^GATC[CT]
              697  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwI        
AspHI       AvaI        AvaII       BanII       BbvI        
BmyI        BpmI        BsaHI       BsaI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrI        BssKI       
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
HaeIII      HgaI        HhaI        Hin1I       HinP1I      
HinfI       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MnlI        MspA1I      MvaI        
MvnI        MwoI        NdeII       NlaIV       PleI        
Sau3AI      Sau96I      ScrFI       StyI        TseI        
Tsp45I      TspRI       
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