(Circular) (Six-base) MAPSORT of: pF1KB6610.seq Check: 4187 from: 1 to: 4403 pF1KB6610 4403 bp With 182 enzymes: SgfI * October 7, 2009 14:21 .. AccI GT'mk_AC Cuts at: 1441 1441 Size: 4403 Acc65I G'GTAC_C Cuts at: 1419 1419 Size: 4403 AclI AA'CG_TT Cuts at: 4243 4243 Size: 4403 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 451 497 2228 2660 3407 3675 451 Size: 46 1731 432 747 268 1179 Fragments arranged by size: 1731 1179 747 432 268 46 AfeI AGC'GCT Cuts at: 140 775 140 Size: 635 3768 AflIII A'CryG_T Cuts at: 1755 2859 3836 1755 Size: 1104 977 2322 Fragments arranged by size: 2322 1104 977 AgeI A'CCGG_T Cuts at: 2791 2791 Size: 4403 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 881 1184 881 Size: 303 4100 AlwNI CAG_nnn'CTG Cuts at: 644 1015 1708 3275 644 Size: 371 693 1567 1772 Fragments arranged by size: 1772 1567 693 371 ApaI G_GGCC'C Cuts at: 441 441 Size: 4403 ApaLI G'TGCA_C Cuts at: 871 3173 871 Size: 2302 2101 ApoI r'AATT_y Cuts at: 340 1407 3756 4012 340 Size: 1067 2349 256 731 Fragments arranged by size: 2349 1067 731 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4403 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4403 AvaI C'yCGr_G Cuts at: 876 1288 1423 876 Size: 412 135 3856 Fragments arranged by size: 3856 412 135 BamHI G'GATC_C Cuts at: 1428 1428 Size: 4403 BanI G'GyrC_C Cuts at: 517 1059 1168 1419 1833 2081 2116 517 Size: 542 109 251 414 248 35 2804 Fragments arranged by size: 2804 542 414 251 248 109 35 BanII G_rGCy'C Cuts at: 195 278 441 586 958 1214 1229 1391 1417 Size: 83 163 145 372 256 15 162 26 Cuts at: 1417 2447 195 Size: 1030 2151 Fragments arranged by size: 2151 1030 372 256 163 162 145 83 26 15 BbeI G_GCGC'C Cuts at: 1063 1837 2085 1063 Size: 774 248 3381 Fragments arranged by size: 3381 774 248 BbsI GAAGACnn'nnnn_ Cuts at: 604 604 Size: 4403 BbvCI CC'TCA_GC Cuts at: 935 949 1049 935 Size: 14 100 4289 Fragments arranged by size: 4289 100 14 BciVI GTATCCnnnnn_n' Cuts at: 2301 3062 4403 2301 Size: 761 1341 2301 Fragments arranged by size: 2301 1341 761 BclI T'GATC_A Cuts at: 1923 1923 Size: 4403 BglI GCCn_nnn'nGGC Cuts at: 112 706 1361 112 Size: 594 655 3154 Fragments arranged by size: 3154 655 594 BglII A'GATC_T Cuts at: 1038 1274 1918 3955 1038 Size: 236 644 2037 1486 Fragments arranged by size: 2037 1486 644 236 BlpI GC'TnA_GC Cuts at: 1515 1515 Size: 4403 Bme1580I G_kGCm'C Cuts at: 441 875 1236 1242 2028 2121 3177 441 Size: 434 361 6 786 93 1056 1667 Fragments arranged by size: 1667 1056 786 434 361 93 6 BmgBI CAC'GTC Cuts at: 408 561 815 408 Size: 153 254 3996 Fragments arranged by size: 3996 254 153 BmrI ACTGGGnnnn_n' Cuts at: 198 571 1615 1696 2031 4175 198 Size: 373 1044 81 335 2144 426 Fragments arranged by size: 2144 1044 426 373 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2770 2770 Size: 4403 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 277 703 742 912 1103 277 Size: 426 39 170 191 3577 Fragments arranged by size: 3577 426 191 170 39 Bpu10I CC'TnA_GC Cuts at: 121 935 949 1049 121 Size: 814 14 100 3475 Fragments arranged by size: 3475 814 100 14 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1008 1579 2458 2950 3248 1008 Size: 571 879 492 298 2163 Fragments arranged by size: 2163 879 571 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 982 1123 4107 982 Size: 141 2984 1278 Fragments arranged by size: 2984 1278 141 BsaAI yAC'GTr Cuts at: 156 1383 1756 156 Size: 1227 373 2803 Fragments arranged by size: 2803 1227 373 BsaBI GATnn'nnATC Cuts at: 6 1917 3954 6 Size: 1911 2037 455 Fragments arranged by size: 2037 1911 455 BsaHI Gr'CG_yC Cuts at: 1060 1834 2082 2784 4280 1060 Size: 774 248 702 1496 1183 Fragments arranged by size: 1496 1183 774 702 248 BsaWI w'CCGG_w Cuts at: 1592 2113 2791 3065 3212 3623 1592 Size: 521 678 274 147 411 2372 Fragments arranged by size: 2372 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 469 499 1090 1120 469 Size: 30 591 30 3752 Fragments arranged by size: 3752 591 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 274 286 481 679 682 718 966 1061 1066 Size: 12 195 198 3 36 248 95 5 Cuts at: 1066 1111 274 Size: 45 3566 Fragments arranged by size: 3566 248 198 195 95 45 36 12 5 3 BseYI C'CCAG_C Cuts at: 1063 1828 2586 3163 3659 1063 Size: 765 758 577 496 1807 Fragments arranged by size: 1807 765 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 743 1105 1177 743 Size: 362 72 3969 Fragments arranged by size: 3969 362 72 BsiEI CG_ry'CG Cuts at: 104 185 1945 1991 3199 104 Size: 81 1760 46 1208 1308 Fragments arranged by size: 1760 1308 1208 81 46 BsiHKAI G_wGCw'C Cuts at: 278 875 1184 1391 1417 2195 2385 3177 278 Size: 597 309 207 26 778 190 792 1504 Fragments arranged by size: 1504 792 778 597 309 207 190 26 Bsp1286I G_dGCh'C Cuts at: 195 278 441 586 875 958 1184 1214 1229 Size: 83 163 145 289 83 226 30 15 Cuts at: 1229 1236 1242 1391 1417 2028 2121 2195 2385 Size: 7 6 149 26 611 93 74 190 Cuts at: 2385 2447 3177 195 Size: 62 730 1421 Fragments arranged by size: 1421 730 611 289 226 190 163 149 145 93 83 83 74 62 30 26 15 7 6 BspEI T'CCGG_A Cuts at: 3623 3623 Size: 4403 BspHI T'CATG_A Cuts at: 3579 3579 Size: 4403 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1453 1667 1969 2350 1453 Size: 214 302 381 3506 Fragments arranged by size: 3506 381 302 214 BsrBI CCG'CTC Cuts at: 50 2695 2749 4233 4398 50 Size: 2645 54 1484 165 55 Fragments arranged by size: 2645 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 225 2315 225 Size: 2090 2313 BsrFI r'CCGG_y Cuts at: 1056 1171 2401 2582 2791 1056 Size: 115 1230 181 209 2668 Fragments arranged by size: 2668 1230 209 181 115 BssSI C'ACGA_G Cuts at: 2674 3032 2674 Size: 358 4045 BstAPI GCAn_nnn'nTGC Cuts at: 1780 1780 Size: 4403 BstBI TT'CG_AA Cuts at: 2764 2764 Size: 4403 BstEII G'GTnAC_C Cuts at: 165 165 Size: 4403 BstXI CCAn_nnnn'nTGG Cuts at: 1383 1383 Size: 4403 BstYI r'GATC_y Cuts at: 1 1038 1274 1428 1895 1918 2253 2499 3511 Size: 1037 236 154 467 23 335 246 1012 Cuts at: 3511 3597 3609 3955 1 Size: 86 12 346 449 Fragments arranged by size: 1037 1012 467 449 346 335 246 236 154 86 23 12 BtgI C'CryG_G Cuts at: 107 416 107 Size: 309 4094 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2353 2534 2697 2353 Size: 181 163 4059 Fragments arranged by size: 4059 181 163 BtsI GCAGTG_nn' Cuts at: 1646 1646 Size: 4403 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 525 560 525 Size: 35 4368 DraI TTT'AAA Cuts at: 1402 1402 Size: 4403 DrdI GACnn_nn'nnGTC Cuts at: 2109 2967 2109 Size: 858 3545 EaeI y'GGCC_r Cuts at: 419 503 546 1353 1988 2162 2553 2580 3925 Size: 84 43 807 635 174 391 27 1345 Cuts at: 3925 419 Size: 897 Fragments arranged by size: 1345 897 807 635 391 174 84 43 27 EagI C'GGCC_G Cuts at: 1988 1988 Size: 4403 EarI CTCTTCn'nnn_ Cuts at: 1088 2426 2636 1088 Size: 1338 210 2855 Fragments arranged by size: 2855 1338 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2611 2921 3067 4213 2611 Size: 310 146 1146 2801 Fragments arranged by size: 2801 1146 310 146 EcoICRI GAG'CTC Cuts at: 276 1389 1415 276 Size: 1113 26 3264 Fragments arranged by size: 3264 1113 26 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 277 451 497 703 742 912 1103 2228 2660 Size: 174 46 206 39 170 191 1125 432 Cuts at: 2660 3407 3675 277 Size: 747 268 1005 Fragments arranged by size: 1125 1005 747 432 268 206 191 174 170 46 39 EcoO109I rG'GnC_Cy Cuts at: 318 437 565 573 1542 318 Size: 119 128 8 969 3179 Fragments arranged by size: 3179 969 128 119 8 EcoRI G'AATT_C Cuts at: 1407 1407 Size: 4403 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2422 2422 Size: 4403 FspI TGC'GCA Cuts at: 2184 2184 Size: 4403 HaeII r_GCGC'y Cuts at: 142 735 777 1063 1837 2085 3107 4086 142 Size: 593 42 286 774 248 1022 979 459 Fragments arranged by size: 1022 979 774 593 459 286 248 42 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 310 342 847 879 2245 2275 2277 2307 310 Size: 32 505 32 1366 30 2 30 2406 Fragments arranged by size: 2406 1366 505 32 32 30 30 2 HincII GTy'rAC Cuts at: 1442 1442 Size: 4403 KasI G'GCGC_C Cuts at: 1059 1833 2081 1059 Size: 774 248 3381 Fragments arranged by size: 3381 774 248 KpnI G_GTAC'C Cuts at: 1423 1423 Size: 4403 MluI A'CGCG_T Cuts at: 3836 3836 Size: 4403 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3074 3258 3074 Size: 184 4219 MscI TGG'CCA Cuts at: 421 505 1355 2164 421 Size: 84 850 809 2660 Fragments arranged by size: 2660 850 809 84 MslI CAynn'nnrTG Cuts at: 229 1312 2392 2519 229 Size: 1083 1080 127 2113 Fragments arranged by size: 2113 1083 1080 127 MspA1I CmG'CkG Cuts at: 284 714 720 1012 1514 1828 2188 3201 3446 Size: 430 6 292 502 314 360 1013 245 Cuts at: 3446 284 Size: 1241 Fragments arranged by size: 1241 1013 502 430 360 314 292 245 6 NaeI GCC'GGC Cuts at: 1058 1173 2584 1058 Size: 115 1411 2877 Fragments arranged by size: 2877 1411 115 NarI GG'CG_CC Cuts at: 1060 1834 2082 1060 Size: 774 248 3381 Fragments arranged by size: 3381 774 248 NcoI C'CATG_G Cuts at: 107 416 107 Size: 309 4094 NgoMIV G'CCGG_C Cuts at: 1056 1171 2582 1056 Size: 115 1411 2877 Fragments arranged by size: 2877 1411 115 NspI r_CATG'y Cuts at: 1368 1456 1956 2863 1368 Size: 88 500 907 2908 Fragments arranged by size: 2908 907 500 88 PasI CC'CwG_GG Cuts at: 1031 1031 Size: 4403 PciI A'CATG_T Cuts at: 2859 2859 Size: 4403 PflMI CCAn_nnn'nTGG Cuts at: 561 561 Size: 4403 PfoI T'CCnGG_A Cuts at: 554 554 Size: 4403 PmeI GTTT'AAAC Cuts at: 1402 1402 Size: 4403 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3542 3574 3542 Size: 32 4371 PpuMI rG'GwC_Cy Cuts at: 318 565 318 Size: 247 4156 PspOMI G'GGCC_C Cuts at: 437 437 Size: 4403 PstI C_TGCA'G Cuts at: 310 968 1450 2135 310 Size: 658 482 685 2578 Fragments arranged by size: 2578 685 658 482 PvuI CG_AT'CG Cuts at: 104 104 Size: 4403 PvuII CAG'CTG Cuts at: 720 1012 1828 2188 720 Size: 292 816 360 2935 Fragments arranged by size: 2935 816 360 292 RsrII CG'GwC_CG Cuts at: 2598 2598 Size: 4403 SacI G_AGCT'C Cuts at: 278 1391 1417 278 Size: 1113 26 3264 Fragments arranged by size: 3264 1113 26 SalI G'TCGA_C Cuts at: 1440 1440 Size: 4403 SanDI GG'GwC_CC Cuts at: 565 565 Size: 4403 SapI GCTCTTCn'nnn_ Cuts at: 2426 2636 2426 Size: 210 4193 SbfI CC_TGCA'GG Cuts at: 1450 1450 Size: 4403 ScaI AGT'ACT Cuts at: 3920 3920 Size: 4403 SfcI C'TryA_G Cuts at: 33 306 525 964 1446 2131 3124 3315 33 Size: 273 219 439 482 685 993 191 1121 Fragments arranged by size: 1121 993 685 482 439 273 219 191 SfoI GGC'GCC Cuts at: 1061 1835 2083 1061 Size: 774 248 3381 Fragments arranged by size: 3381 774 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4403 SmaI CCC'GGG Cuts at: 1425 1425 Size: 4403 SmlI C'TyrA_G Cuts at: 1023 1558 2473 2965 3227 1023 Size: 535 915 492 262 2199 Fragments arranged by size: 2199 915 535 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4403 SphI G_CATG'C Cuts at: 1456 1956 1456 Size: 500 3903 StuI AGG'CCT Cuts at: 151 151 Size: 4403 StyI C'CwwG_G Cuts at: 107 416 506 1537 1891 107 Size: 309 90 1031 354 2619 Fragments arranged by size: 2619 1031 354 309 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 199 994 1986 2119 2578 2787 199 Size: 795 992 133 459 209 1815 Fragments arranged by size: 1815 992 795 459 209 133 TatI w'GTAC_w Cuts at: 3918 3918 Size: 4403 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 469 501 3920 3952 469 Size: 32 3419 32 920 Fragments arranged by size: 3419 920 32 32 Tth111I GACn'n_nGTC Cuts at: 2200 2200 Size: 4403 XbaI T'CTAG_A Cuts at: 1434 1434 Size: 4403 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1213 1898 1213 Size: 685 3718 XmaI C'CCGG_G Cuts at: 1423 1423 Size: 4403 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AflII AhdI AleI AloI AscI AvrII BaeI BcgI BfrBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI