Restriction map for pF1KB6606
##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvrII       BbrPI       BbvCI       BciVI       
BclI        BfaI        BfrI        BlnI        BlpI        
Bpu1102I    BsaAI       BseAI       BsiEI       BsiWI       
BsmI        BspDI       BspEI       BspLU11I    BsrDI       
BssHII      BssSI       Bst1107I    BstBI       BstUI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HindIII     HpaI        
KpnI        KspI        MaeI        MfeI        MluI        
MroI        MseI        MunI        MvnI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SapI        ScaI        SfuI        
SgrAI       SnaBI       SpeI        SphI        SspI        
SwaI        Tru9I       Tth111I     Van91I      XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AcsI          283  AAATTT               [GA]^AATT[CT]
AfaI          384  GTAC                 GT^AC
ApaI          864  GGGCCC               GGGCC^C
ApoI          283  AAATTT               [GA]^AATT[CT]
AviII         341  TGCGCA               TGC^GCA
BalI          677  TGGCCA               TGG^CCA
BamHI        1118  GGATCC               G^GATCC
BbeI          234  GGCGCC               GGCGC^C
BcgI          260  CGAGCTTCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          820  GCCGCCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         554  AGATCT               A^GATCT
BsaBI         549  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1135  GAGACC               GGTCTC(1/5)
BsaWI         174  ACCGGA               [AT]^CCGG[AT]
Bsp1407I      383  TGTACA               T^GTACA
BsrBI         367  GAGCGG               CCGCTC(-3/-3)
BsrFI         104  GCCGGC               [GA]^CCGG[CT]
BsrGI         383  TGTACA               T^GTACA
BstEII        723  GGTCACC              G^GT[ACGT]ACC
BstPI         723  GGTCACC              G^GT[ACGT]ACC
Cfr10I        104  GCCGGC               [GA]^CCGG[CT]
EaeI          677  TGGCCA               [CT]^GGCC[GA]
EarI          952  GAAGAG               CTCTTC(1/4)
EcoO65I       723  GGTCACC              G^GT[ACGT]ACC
FspI          341  TGCGCA               TGC^GCA
HaeII         234  GGCGCC               [GA]GCGC^[CT]
HincII        628  GTCAAC               GT[CT]^[GA]AC
HindII        628  GTCAAC               GT[CT]^[GA]AC
HphI          725  TCACC                GGTGA(8/7)
KasI          234  GGCGCC               G^GCGCC
Ksp632I       952  GAAGAG               CTCTTC(1/4)
MaeII         626  ACGT                 A^CGT
MaeIII        724  GTCAC                ^GT[ACGT]AC
MamI          549  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         677  TGGCCA               TGG^CCA
MscI          677  TGGCCA               TGG^CCA
MslI         1074  CATGTGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          104  GCCGGC               GCC^GGC
NarI          234  GGCGCC               GG^CGCC
NgoMIV        104  GCCGGC               G^CCGGC
RsaI          384  GTAC                 GT^AC
SacI         1195  GAGCTC               GAGCT^C
SbfI         1128  CCTGCAGG             CCTGCA^GG
SexAI         734  ACCAGGT              A^CC[AT]GGT
SfiI          819  GGCCGCCCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          234  GGCGCC               GGC^GCC
SmlI          583  CTCAAG               C^T[CT][GA]AG
Sse8387I     1128  CCTGCAGG             CCTGCA^GG
SspBI         383  TGTACA               T^GTACA
StuI           33  AGGCCT               AGG^CCT
Tsp45I        724  GTCAC                ^GT[GC]AC
Tsp509I       284  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcyI          234  GGCGCC               G[GA]^CG[CT]C
              957  GACGCC              
AlwNI         154  CAGTCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1123  CAGGGCCTG           
BanII         864  GGGCCC               G[GA]GC[CT]^C
             1195  GAGCTC              
BsaHI         234  GGCGCC               G[GA]^CG[CT]C
              957  GACGCC              
BsgI          277  GTGCAG               GTGCAG(16/14)
              764  GTGCAG              
BsmBI         532  GAGACG               CGTCTC(1/5)
              955  GAGACG              
BspHI         620  TCATGA               T^CATGA
             1092  TCATGA              
BsrI           24  ACTGG                ACTGG(1/-1)
              640  CCAGT               
BstAPI        270  GCAGACGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              294  GCACAGCGTGC         
DraIII        295  CACAGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
              511  CACGGTGTG           
DsaI          510  CCACGG               C^C[GA][CT]GG
             1163  CCGTGG              
EcoNI         654  CCTGCCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1233  CCTGCCCGAGG         
EcoT14I       197  CCAAGG               C^C[AT][AT]GG
              410  CCAAGG              
HgaI          563  GACGC                GACGC(5/10)
              957  GACGC               
Hin1I         234  GGCGCC               G[GA]^CG[CT]C
              957  GACGCC              
PleI          754  GACTC                GAGTC(4/5)
              804  GAGTC               
RcaI          620  TCATGA               T^CATGA
             1092  TCATGA              
SmaI          647  CCCGGG               CCC^GGG
             1190  CCCGGG              
StyI          197  CCAAGG               C^C[AT][AT]GG
              410  CCAAGG              
TaqI          365  TCGA                 T^CGA
              615  TCGA                
TspRI         124  CACTG                CA[GC]TG[ACGT][ACGT]^
              641  CAGTG               
TthHB8I       365  TCGA                 T^CGA
              615  TCGA                
XmaCI         647  CCCGGG               C^CCGGG
             1190  CCCGGG              
XmaI          647  CCCGGG               C^CCGGG
             1190  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AciI        AluI        AlwI        AspHI       AvaI        
AvaII       BanI        BbsI        BbvI        BcnI        
BmyI        BpmI        BpuAI       BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BssKI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
ItaI        MboI        MboII       MflI        MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PpuMI       PstI        
PvuII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TfiI        TseI        XcmI        XhoII       
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