Restriction map for
pF1KB6606
(Linear) (Six-base) MAPSORT of: KIBB6606.seq Check: 4340 from: 1 to: 1293
KIBB6606.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 67 1293
Size: 67 1226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 1293
Size: 619 674
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 302 1293
Size: 302 991
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 523 555 1293
Size: 523 32 738
Fragments arranged by size:
738 523 32
AlwNI CAG_nnn'CTG
Cuts at: 0 159 1128 1293
Size: 159 969 165
Fragments arranged by size:
969 165 159
ApaI G_GGCC'C
Cuts at: 0 868 1293
Size: 868 425
ApoI r'AATT_y
Cuts at: 0 283 1293
Size: 283 1010
AvaI C'yCGr_G
Cuts at: 0 29 113 647 1190 1237 1293
Size: 29 84 534 543 47 56
Fragments arranged by size:
543 534 84 56 47 29
BamHI G'GATC_C
Cuts at: 0 1118 1293
Size: 1118 175
BanI G'GyrC_C
Cuts at: 0 55 207 234 1293
Size: 55 152 27 1059
Fragments arranged by size:
1059 152 55 27
BanII G_rGCy'C
Cuts at: 0 868 1199 1293
Size: 868 331 94
Fragments arranged by size:
868 331 94
BbeI G_GCGC'C
Cuts at: 0 238 1293
Size: 238 1055
BbsI GAAGACnn'nnnn_
Cuts at: 0 411 1059 1161 1262 1293
Size: 411 648 102 101 31
Fragments arranged by size:
648 411 102 101 31
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 249 283 1293
Size: 249 34 1010
Fragments arranged by size:
1010 249 34
BglI GCCn_nnn'nGGC
Cuts at: 0 826 1293
Size: 826 467
BglII A'GATC_T
Cuts at: 0 554 1293
Size: 554 739
Bme1580I G_kGCm'C
Cuts at: 0 212 647 868 1293
Size: 212 435 221 425
Fragments arranged by size:
435 425 221 212
BmrI ACTGGGnnnn_n'
Cuts at: 0 634 1293
Size: 634 659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 1021 1183 1240 1293
Size: 514 507 162 57 53
Fragments arranged by size:
514 507 162 57 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 1293
Size: 568 725
BsaI GGTCTCn'nnnn_
Cuts at: 0 1129 1293
Size: 1129 164
BsaBI GATnn'nnATC
Cuts at: 0 553 1293
Size: 553 740
BsaHI Gr'CG_yC
Cuts at: 0 235 958 1293
Size: 235 723 335
Fragments arranged by size:
723 335 235
BsaWI w'CCGG_w
Cuts at: 0 174 1293
Size: 174 1119
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 523 553 1293
Size: 523 30 740
Fragments arranged by size:
740 523 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 952 979 1026 1237 1293
Size: 952 27 47 211 56
Fragments arranged by size:
952 211 56 47 27
BseYI C'CCAG_C
Cuts at: 0 5 166 779 1019 1293
Size: 5 161 613 240 274
Fragments arranged by size:
613 274 240 161 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 785 1293
Size: 298 487 508
Fragments arranged by size:
508 487 298
BsiHKAI G_wGCw'C
Cuts at: 0 296 1092 1199 1293
Size: 296 796 107 94
Fragments arranged by size:
796 296 107 94
BsmBI CGTCTCn'nnnn_
Cuts at: 0 526 949 1293
Size: 526 423 344
Fragments arranged by size:
526 423 344
Bsp1286I G_dGCh'C
Cuts at: 0 212 296 647 868 1092 1199 1293
Size: 212 84 351 221 224 107 94
Fragments arranged by size:
351 224 221 212 107 94 84
BspHI T'CATG_A
Cuts at: 0 620 1092 1293
Size: 620 472 201
Fragments arranged by size:
620 472 201
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 67 662 1274 1293
Size: 67 595 612 19
Fragments arranged by size:
612 595 67 19
BsrBI CCG'CTC
Cuts at: 0 369 1293
Size: 369 924
BsrFI r'CCGG_y
Cuts at: 0 104 1293
Size: 104 1189
BsrGI T'GTAC_A
Cuts at: 0 383 1293
Size: 383 910
BstAPI GCAn_nnn'nTGC
Cuts at: 0 276 300 1293
Size: 276 24 993
Fragments arranged by size:
993 276 24
BstEII G'GTnAC_C
Cuts at: 0 723 1293
Size: 723 570
BstXI CCAn_nnnn'nTGG
Cuts at: 0 24 204 1080 1293
Size: 24 180 876 213
Fragments arranged by size:
876 213 180 24
BstYI r'GATC_y
Cuts at: 0 554 771 1118 1157 1293
Size: 554 217 347 39 136
Fragments arranged by size:
554 347 217 136 39
BtgI C'CryG_G
Cuts at: 0 510 1163 1293
Size: 510 653 130
Fragments arranged by size:
653 510 130
BtsI GCAGTG_nn'
Cuts at: 0 123 1293
Size: 123 1170
DraIII CAC_nnn'GTG
Cuts at: 0 300 516 1293
Size: 300 216 777
Fragments arranged by size:
777 300 216
EaeI y'GGCC_r
Cuts at: 0 677 1293
Size: 677 616
EarI CTCTTCn'nnn_
Cuts at: 0 947 1293
Size: 947 346
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1162 1293
Size: 1162 131
EcoICRI GAG'CTC
Cuts at: 0 1197 1293
Size: 1197 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 619 1021 1183 1240 1293
Size: 514 105 402 162 57 53
Fragments arranged by size:
514 402 162 105 57 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 658 1237 1293
Size: 658 579 56
Fragments arranged by size:
658 579 56
EcoO109I rG'GnC_Cy
Cuts at: 0 44 96 117 219 372 651 663 864
Size: 44 52 21 102 153 279 12 201
Cuts at: 864 865 1060 1125 1293
Size: 1 195 65 168
Fragments arranged by size:
279 201 195 168 153 102 65 52
44 21 12 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 279 435 1293
Size: 279 156 858
Fragments arranged by size:
858 279 156
FspI TGC'GCA
Cuts at: 0 343 1293
Size: 343 950
HaeII r_GCGC'y
Cuts at: 0 238 1293
Size: 238 1055
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 795 827 1293
Size: 795 32 466
Fragments arranged by size:
795 466 32
HincII GTy'rAC
Cuts at: 0 630 1293
Size: 630 663
KasI G'GCGC_C
Cuts at: 0 234 1293
Size: 234 1059
MscI TGG'CCA
Cuts at: 0 679 1293
Size: 679 614
MslI CAynn'nnrTG
Cuts at: 0 1078 1293
Size: 1078 215
MspA1I CmG'CkG
Cuts at: 0 136 393 993 1293
Size: 136 257 600 300
Fragments arranged by size:
600 300 257 136
NaeI GCC'GGC
Cuts at: 0 106 1293
Size: 106 1187
NarI GG'CG_CC
Cuts at: 0 235 1293
Size: 235 1058
NgoMIV G'CCGG_C
Cuts at: 0 104 1293
Size: 104 1189
PfoI T'CCnGG_A
Cuts at: 0 491 1293
Size: 491 802
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 523 555 1293
Size: 523 32 738
Fragments arranged by size:
738 523 32
PpuMI rG'GwC_Cy
Cuts at: 0 96 219 372 651 663 1293
Size: 96 123 153 279 12 630
Fragments arranged by size:
630 279 153 123 96 12
PspOMI G'GGCC_C
Cuts at: 0 864 1293
Size: 864 429
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 964 996 1293
Size: 964 32 297
Fragments arranged by size:
964 297 32
PstI C_TGCA'G
Cuts at: 0 130 272 1133 1215 1271 1293
Size: 130 142 861 82 56 22
Fragments arranged by size:
861 142 130 82 56 22
PvuII CAG'CTG
Cuts at: 0 136 393 993 1293
Size: 136 257 600 300
Fragments arranged by size:
600 300 257 136
SacI G_AGCT'C
Cuts at: 0 1199 1293
Size: 1199 94
SbfI CC_TGCA'GG
Cuts at: 0 1133 1293
Size: 1133 160
SexAI A'CCwGG_T
Cuts at: 0 734 1293
Size: 734 559
SfcI C'TryA_G
Cuts at: 0 39 126 268 1129 1211 1267 1293
Size: 39 87 142 861 82 56 26
Fragments arranged by size:
861 142 87 82 56 39 26
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 826 1293
Size: 826 467
SfoI GGC'GCC
Cuts at: 0 236 1293
Size: 236 1057
SmaI CCC'GGG
Cuts at: 0 649 1192 1293
Size: 649 543 101
Fragments arranged by size:
649 543 101
SmlI C'TyrA_G
Cuts at: 0 583 1293
Size: 583 710
StuI AGG'CCT
Cuts at: 0 35 1293
Size: 35 1258
StyI C'CwwG_G
Cuts at: 0 197 410 1293
Size: 197 213 883
Fragments arranged by size:
883 213 197
TatI w'GTAC_w
Cuts at: 0 383 1293
Size: 383 910
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 201 585 1146 1293
Size: 201 384 561 147
Fragments arranged by size:
561 384 201 147
XmaI C'CCGG_G
Cuts at: 0 647 1190 1293
Size: 647 543 103
Fragments arranged by size:
647 543 103
Enzymes that do cut:
AarI AcuI AlfI AloI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbeI BbsI BcgI BglI BglII
Bme1580I BmrI BpmI BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrFI BsrGI BstAPI BstEII BstXI BstYI
BtgI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FspI HaeII Hin4I HincII KasI
MscI MslI MspA1I NaeI NarI NgoMIV PfoI PpiI
PpuMI PspOMI PsrI PstI PvuII SacI SbfI SexAI
SfcI SfiI SfoI SmaI SmlI StuI StyI TatI
XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI ApaLI AscI AseI AsiSI AvrII BaeI
BbvCI BciVI BclI BfrBI BlpI BmgBI BmtI BplI
Bpu10I BsaAI BsiEI BsiWI BsmI BspEI BsrDI BssHII
BssSI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EagI EcoRI EcoRV FseI FspAI HindIII HpaI
KpnI MfeI MluI MmeI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PshAI PsiI PspXI PvuI RsrII SacII SalI
SanDI SapI ScaI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TstI Tth111I XbaI XhoI
XmnI ZraI