Restriction map for
pF1KB6578
(Circular) (Six-base) MAPSORT of: pF1KB6578.seq Check: 6191 from: 1 to: 4319
pF1KB6578 4319 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1034 1034
Size: 4319
AatII G_ACGT'C
Cuts at: 380 380
Size: 4319
AccI GT'mk_AC
Cuts at: 1357 1357
Size: 4319
Acc65I G'GTAC_C
Cuts at: 1335 1335
Size: 4319
AclI AA'CG_TT
Cuts at: 4159 4159
Size: 4319
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 852 1001 2144 2576 3323 3591 852
Size: 149 1143 432 747 268 1580
Fragments arranged by size:
1580 1143 747 432 268 149
AfeI AGC'GCT
Cuts at: 910 910
Size: 4319
AflIII A'CryG_T
Cuts at: 1671 2775 3752 1671
Size: 1104 977 2238
Fragments arranged by size:
2238 1104 977
AgeI A'CCGG_T
Cuts at: 2707 2707
Size: 4319
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 422 454 422
Size: 32 4287
AlwNI CAG_nnn'CTG
Cuts at: 192 1052 1200 1624 3191 192
Size: 860 148 424 1567 1320
Fragments arranged by size:
1567 1320 860 424 148
ApaI G_GGCC'C
Cuts at: 155 525 1159 1265 155
Size: 370 634 106 3209
Fragments arranged by size:
3209 634 370 106
ApaLI G'TGCA_C
Cuts at: 550 729 3089 550
Size: 179 2360 1780
Fragments arranged by size:
2360 1780 179
ApoI r'AATT_y
Cuts at: 1323 3672 3928 1323
Size: 2349 256 1714
Fragments arranged by size:
2349 1714 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4319
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4319
AvaI C'yCGr_G
Cuts at: 154 1257 1339 154
Size: 1103 82 3134
Fragments arranged by size:
3134 1103 82
BamHI G'GATC_C
Cuts at: 690 1344 690
Size: 654 3665
BanI G'GyrC_C
Cuts at: 266 1335 1749 1997 2032 266
Size: 1069 414 248 35 2553
Fragments arranged by size:
2553 1069 414 248 35
BanII G_rGCy'C
Cuts at: 155 525 742 962 1139 1159 1265 1333 2363
Size: 370 217 220 177 20 106 68 1030
Cuts at: 2363 155
Size: 2111
Fragments arranged by size:
2111 1030 370 220 217 177 106 68
20
BbeI G_GCGC'C
Cuts at: 1753 2001 1753
Size: 248 4071
BbsI GAAGACnn'nnnn_
Cuts at: 1077 1077
Size: 4319
BciVI GTATCCnnnnn_n'
Cuts at: 2217 2978 4319 2217
Size: 761 1341 2217
Fragments arranged by size:
2217 1341 761
BclI T'GATC_A
Cuts at: 1098 1839 1098
Size: 741 3578
BglI GCCn_nnn'nGGC
Cuts at: 602 1061 1143 602
Size: 459 82 3778
Fragments arranged by size:
3778 459 82
BglII A'GATC_T
Cuts at: 1834 3871 1834
Size: 2037 2282
BlpI GC'TnA_GC
Cuts at: 1431 1431
Size: 4319
Bme1580I G_kGCm'C
Cuts at: 155 269 525 554 733 1159 1242 1265 1944
Size: 114 256 29 179 426 83 23 679
Cuts at: 1944 2037 3093 155
Size: 93 1056 1381
Fragments arranged by size:
1381 1056 679 426 256 179 114 93
83 29 23
BmrI ACTGGGnnnn_n'
Cuts at: 564 1267 1531 1612 1947 4091 564
Size: 703 264 81 335 2144 792
Fragments arranged by size:
2144 792 703 335 264 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1236 2686 1236
Size: 1450 2869
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 613
Size: 4319
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1495 2374 2866 3164 1495
Size: 879 492 298 2650
Fragments arranged by size:
2650 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 785 4023 785
Size: 3238 1081
BsaAI yAC'GTr
Cuts at: 1672 1672
Size: 4319
BsaBI GATnn'nnATC
Cuts at: 6 1833 3870 6
Size: 1827 2037 455
Fragments arranged by size:
2037 1827 455
BsaHI Gr'CG_yC
Cuts at: 377 1750 1998 2700 4196 377
Size: 1373 248 702 1496 500
Fragments arranged by size:
1496 1373 702 500 248
BsaWI w'CCGG_w
Cuts at: 1508 2029 2707 2981 3128 3539 1508
Size: 521 678 274 147 411 2288
Fragments arranged by size:
2288 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 472 502 472
Size: 30 4289
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 301 313 474 493 529 301
Size: 12 161 19 36 4091
Fragments arranged by size:
4091 161 36 19 12
BseYI C'CCAG_C
Cuts at: 1158 1163 1167 1744 2502 3079 3575 1158
Size: 5 4 577 758 577 496 1902
Fragments arranged by size:
1902 758 577 577 496 5 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 440 914 440
Size: 474 3845
BsiEI CG_ry'CG
Cuts at: 104 255 1861 1907 3115 104
Size: 151 1606 46 1208 1308
Fragments arranged by size:
1606 1308 1208 151 46
BsiHKAI G_wGCw'C
Cuts at: 554 733 1235 1333 2111 2301 3093 554
Size: 179 502 98 778 190 792 1780
Fragments arranged by size:
1780 792 778 502 190 179 98
Bsp1286I G_dGCh'C
Cuts at: 155 269 525 554 733 742 962 1139 1159
Size: 114 256 29 179 9 220 177 20
Cuts at: 1159 1235 1242 1265 1333 1944 2037 2111 2301
Size: 76 7 23 68 611 93 74 190
Cuts at: 2301 2363 3093 155
Size: 62 730 1381
Fragments arranged by size:
1381 730 611 256 220 190 179 177
114 93 76 74 68 62 29 23
20 9 7
BspEI T'CCGG_A
Cuts at: 3539 3539
Size: 4319
BspHI T'CATG_A
Cuts at: 3495 3495
Size: 4319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1034 1179 1369 1583 1885 2266 1034
Size: 145 190 214 302 381 3087
Fragments arranged by size:
3087 381 302 214 190 145
BsrBI CCG'CTC
Cuts at: 50 519 2611 2665 4149 4314 50
Size: 469 2092 54 1484 165 55
Fragments arranged by size:
2092 1484 469 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2231 2231
Size: 4319
BsrFI r'CCGG_y
Cuts at: 2317 2498 2707 2317
Size: 181 209 3929
Fragments arranged by size:
3929 209 181
BssHII G'CGCG_C
Cuts at: 291 291
Size: 4319
BssSI C'ACGA_G
Cuts at: 1132 2590 2948 1132
Size: 1458 358 2503
Fragments arranged by size:
2503 1458 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1052 1696 1052
Size: 644 3675
BstBI TT'CG_AA
Cuts at: 2680 2680
Size: 4319
BstXI CCAn_nnnn'nTGG
Cuts at: 369 933 369
Size: 564 3755
BstYI r'GATC_y
Cuts at: 1 690 1344 1811 1834 2169 2415 3427 3513
Size: 689 654 467 23 335 246 1012 86
Cuts at: 3513 3525 3871 1
Size: 12 346 449
Fragments arranged by size:
1012 689 654 467 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2269 2450 2613 2269
Size: 181 163 3975
Fragments arranged by size:
3975 181 163
BtsI GCAGTG_nn'
Cuts at: 410 1053 1562 410
Size: 643 509 3167
Fragments arranged by size:
3167 643 509
ClaI AT'CG_AT
Cuts at: 809 809
Size: 4319
DraI TTT'AAA
Cuts at: 1318 1318
Size: 4319
DrdI GACnn_nn'nnGTC
Cuts at: 2025 2883 2025
Size: 858 3461
EaeI y'GGCC_r
Cuts at: 183 413 1286 1904 2078 2469 2496 3841 183
Size: 230 873 618 174 391 27 1345 661
Fragments arranged by size:
1345 873 661 618 391 230 174 27
EagI C'GGCC_G
Cuts at: 1904 1904
Size: 4319
EarI CTCTTCn'nnn_
Cuts at: 506 1210 1273 2342 2552 506
Size: 704 63 1069 210 2273
Fragments arranged by size:
2273 1069 704 210 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 102 265 298 388 2527 2837 2983 4129 102
Size: 163 33 90 2139 310 146 1146 292
Fragments arranged by size:
2139 1146 310 292 163 146 90 33
EcoICRI GAG'CTC
Cuts at: 1331 1331
Size: 4319
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 852 1001 2144 2576 3323 3591 613
Size: 239 149 1143 432 747 268 1341
Fragments arranged by size:
1341 1143 747 432 268 239 149
EcoO109I rG'GnC_Cy
Cuts at: 1155 1261 1262 1458 1155
Size: 106 1 196 4016
Fragments arranged by size:
4016 196 106 1
EcoRI G'AATT_C
Cuts at: 1323 1323
Size: 4319
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2338 2338
Size: 4319
FspI TGC'GCA
Cuts at: 2100 2100
Size: 4319
HaeII r_GCGC'y
Cuts at: 568 912 1753 2001 3023 4002 568
Size: 344 841 248 1022 979 885
Fragments arranged by size:
1022 979 885 841 344 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2161 2191 2193 2223 2161
Size: 30 2 30 4257
Fragments arranged by size:
4257 30 30 2
HincII GTy'rAC
Cuts at: 1358 1358
Size: 4319
KasI G'GCGC_C
Cuts at: 1749 1997 1749
Size: 248 4071
KpnI G_GTAC'C
Cuts at: 1339 1339
Size: 4319
MfeI C'AATT_G
Cuts at: 838 838
Size: 4319
MluI A'CGCG_T
Cuts at: 3752 3752
Size: 4319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 683 2990 3174 683
Size: 2307 184 1828
Fragments arranged by size:
2307 1828 184
MscI TGG'CCA
Cuts at: 415 2080 415
Size: 1665 2654
MslI CAynn'nnrTG
Cuts at: 620 2308 2435 620
Size: 1688 127 2504
Fragments arranged by size:
2504 1688 127
MspA1I CmG'CkG
Cuts at: 444 486 546 633 1167 1430 1744 2104 3117
Size: 42 60 87 534 263 314 360 1013
Cuts at: 3117 3362 444
Size: 245 1401
Fragments arranged by size:
1401 1013 534 360 314 263 245 87
60 42
NaeI GCC'GGC
Cuts at: 2500 2500
Size: 4319
NarI GG'CG_CC
Cuts at: 1750 1998 1750
Size: 248 4071
NgoMIV G'CCGG_C
Cuts at: 2498 2498
Size: 4319
NspI r_CATG'y
Cuts at: 609 1372 1872 2779 609
Size: 763 500 907 2149
Fragments arranged by size:
2149 907 763 500
PasI CC'CwG_GG
Cuts at: 1140 1140
Size: 4319
PciI A'CATG_T
Cuts at: 2775 2775
Size: 4319
PmeI GTTT'AAAC
Cuts at: 1318 1318
Size: 4319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3458 3490 3458
Size: 32 4287
PshAI GACnn'nnGTC
Cuts at: 1055 1055
Size: 4319
PspOMI G'GGCC_C
Cuts at: 151 521 1155 1261 151
Size: 370 634 106 3209
Fragments arranged by size:
3209 634 370 106
PstI C_TGCA'G
Cuts at: 341 572 1366 2051 341
Size: 231 794 685 2609
Fragments arranged by size:
2609 794 685 231
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4319
PvuII CAG'CTG
Cuts at: 633 1167 1744 2104 633
Size: 534 577 360 2848
Fragments arranged by size:
2848 577 534 360
RsrII CG'GwC_CG
Cuts at: 2514 2514
Size: 4319
SacI G_AGCT'C
Cuts at: 1333 1333
Size: 4319
SalI G'TCGA_C
Cuts at: 1356 1356
Size: 4319
SapI GCTCTTCn'nnn_
Cuts at: 1210 2342 2552 1210
Size: 1132 210 2977
Fragments arranged by size:
2977 1132 210
SbfI CC_TGCA'GG
Cuts at: 1366 1366
Size: 4319
ScaI AGT'ACT
Cuts at: 3836 3836
Size: 4319
SfcI C'TryA_G
Cuts at: 33 337 568 1362 2047 3040 3231 33
Size: 304 231 794 685 993 191 1121
Fragments arranged by size:
1121 993 794 685 304 231 191
SfoI GGC'GCC
Cuts at: 1751 1999 1751
Size: 248 4071
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4319
SmaI CCC'GGG
Cuts at: 156 1259 1341 156
Size: 1103 82 3134
Fragments arranged by size:
3134 1103 82
SmlI C'TyrA_G
Cuts at: 1474 2389 2881 3143 1474
Size: 915 492 262 2650
Fragments arranged by size:
2650 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4319
SphI G_CATG'C
Cuts at: 609 1372 1872 609
Size: 763 500 3056
Fragments arranged by size:
3056 763 500
SrfI GCCC'GGGC
Cuts at: 156 156
Size: 4319
StuI AGG'CCT
Cuts at: 889 889
Size: 4319
StyI C'CwwG_G
Cuts at: 1453 1807 1453
Size: 354 3965
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 682 1159 1902 2035 2494 2703 682
Size: 477 743 133 459 209 2298
Fragments arranged by size:
2298 743 477 459 209 133
TatI w'GTAC_w
Cuts at: 3834 3834
Size: 4319
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3836 3868 3836
Size: 32 4287
Tth111I GACn'n_nGTC
Cuts at: 2116 2116
Size: 4319
XbaI T'CTAG_A
Cuts at: 1350 1350
Size: 4319
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1814 1814
Size: 4319
XmaI C'CCGG_G
Cuts at: 154 1257 1339 154
Size: 1103 82 3134
Fragments arranged by size:
3134 1103 82
ZraI GAC'GTC
Cuts at: 378 378
Size: 4319
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PmeI PpiI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AleI AlfI AscI AvrII BaeI BbvCI
BcgI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI BsmBI
BsrGI BstEII BstZ17I Bsu36I BtgI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI NcoI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PmlI PpuMI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SspI SwaI XhoI XmnI