Restriction map for pF1KB6566
 (Circular) (Six-base) MAPSORT of: pF1KB6566.seq  Check: 9528  from: 1  to: 4313



pF1KB6566  4313 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

AccI GT'mk_AC

Cuts at:   1351    1351 
   Size:       4313
 
Acc65I G'GTAC_C

Cuts at:    452    1329     452 
   Size:        877    3436
 
AclI AA'CG_TT

Cuts at:   4153    4153 
   Size:       4313
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    748     769     929     953    1000    2138    2570    3317    3585 
   Size:         21     160      24      47    1138     432     747     268

Cuts at:   3585     748 
   Size:       1476

  Fragments arranged by size: 

                1476    1138     747     432     268     160      47      24
                  21
 
AflIII A'CryG_T

Cuts at:   1665    2769    3746    1665 
   Size:       1104     977    2232

  Fragments arranged by size: 

                2232    1104     977
 
AgeI A'CCGG_T

Cuts at:   2701    2701 
   Size:       4313
 
AhdI GACnn_n'nnGTC

Cuts at:   1215    1215 
   Size:       4313
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1253    1253 
   Size:       4313
 
AlwNI CAG_nnn'CTG

Cuts at:    516     630    1128    1618    3185     516 
   Size:        114     498     490    1567    1644

  Fragments arranged by size: 

                1644    1567     498     490     114
 
ApaI G_GGCC'C

Cuts at:    476     476 
   Size:       4313
 
ApaLI G'TGCA_C

Cuts at:   3083    3083 
   Size:       4313
 
ApoI r'AATT_y

Cuts at:   1317    3666    3922    1317 
   Size:       2349     256    1708

  Fragments arranged by size: 

                2349    1708     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4313
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4313
 
AvaI C'yCGr_G

Cuts at:    279    1258    1333     279 
   Size:        979      75    3259

  Fragments arranged by size: 

                3259     979      75
 
BamHI G'GATC_C

Cuts at:   1338    1338 
   Size:       4313
 
BanI G'GyrC_C

Cuts at:    452     535     832    1329    1743    1991    2026     452 
   Size:         83     297     497     414     248      35    2739

  Fragments arranged by size: 

                2739     497     414     297     248      83      35
 
BanII G_rGCy'C

Cuts at:    226     476    1107    1327    2357     226 
   Size:        250     631     220    1030    2182

  Fragments arranged by size: 

                2182    1030     631     250     220
 
BbeI G_GCGC'C

Cuts at:   1747    1995    1747 
   Size:        248    4065
 
BbsI GAAGACnn'nnnn_

Cuts at:   1076    1076 
   Size:       4313
 
BbvCI CC'TCA_GC

Cuts at:    781     781 
   Size:       4313
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    822     856    1239    1273     822 
   Size:         34     383      34    3862

  Fragments arranged by size: 

                3862     383      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:    252    2211    2972    4313     252 
   Size:       1959     761    1341     252

  Fragments arranged by size: 

                1959    1341     761     252
 
BclI T'GATC_A

Cuts at:   1130    1833    1130 
   Size:        703    3610
 
BglI GCCn_nnn'nGGC

Cuts at:    164     412     586    1111     164 
   Size:        248     174     525    3366

  Fragments arranged by size: 

                3366     525     248     174
 
BglII A'GATC_T

Cuts at:   1828    3865    1828 
   Size:       2037    2276
 
BlpI GC'TnA_GC

Cuts at:   1048    1425    1048 
   Size:        377    3936
 
Bme1580I G_kGCm'C

Cuts at:    476     835    1938    2031    3087     476 
   Size:        359    1103      93    1056    1702

  Fragments arranged by size: 

                1702    1103    1056     359      93
 
BmgBI CAC'GTC

Cuts at:    143     143 
   Size:       4313
 
BmrI ACTGGGnnnn_n'

Cuts at:   1525    1606    1941    4085    1525 
   Size:         81     335    2144    1753

  Fragments arranged by size: 

                2144    1753     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2680    2680 
   Size:       4313
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    430     537     727     958    1246     430 
   Size:        107     190     231     288    3497

  Fragments arranged by size: 

                3497     288     231     190     107
 
Bpu10I CC'TnA_GC

Cuts at:    781     781 
   Size:       4313
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1045    1489    2368    2860    3158    1045 
   Size:        444     879     492     298    2200

  Fragments arranged by size: 

                2200     879     492     444     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4017    4017 
   Size:       4313
 
BsaAI yAC'GTr

Cuts at:   1666    1666 
   Size:       4313
 
BsaBI GATnn'nnATC

Cuts at:      6     715    1827    3864       6 
   Size:        709    1112    2037     455

  Fragments arranged by size: 

                2037    1112     709     455
 
BsaHI Gr'CG_yC

Cuts at:    308    1744    1992    2694    4190     308 
   Size:       1436     248     702    1496     431

  Fragments arranged by size: 

                1496    1436     702     431     248
 
BsaWI w'CCGG_w

Cuts at:    896    1502    2023    2701    2975    3122    3533     896 
   Size:        606     521     678     274     147     411    1676

  Fragments arranged by size: 

                1676     678     606     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    208     238     788     818     208 
   Size:         30     550      30    3703

  Fragments arranged by size: 

                3703     550      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    267     778     267 
   Size:        511    3802
 
BseYI C'CCAG_C

Cuts at:    208    1106    1148    1152    1738    2496    3073    3569     208 
   Size:        898      42       4     586     758     577     496     952

  Fragments arranged by size: 

                 952     898     758     586     577     496      42       4
            
 
BsiEI CG_ry'CG

Cuts at:    104     234     375    1855    1901    3109     104 
   Size:        130     141    1480      46    1208    1308

  Fragments arranged by size: 

                1480    1308    1208     141     130      46
 
BsiHKAI G_wGCw'C

Cuts at:   1327    2105    2295    3087    1327 
   Size:        778     190     792    2553

  Fragments arranged by size: 

                2553     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    212     212 
   Size:       4313
 
BsmBI CGTCTCn'nnnn_

Cuts at:    610     610 
   Size:       4313
 
Bsp1286I G_dGCh'C

Cuts at:    226     476     835    1107    1327    1938    2031    2105    2295 
   Size:        250     359     272     220     611      93      74     190

Cuts at:   2295    2357    3087     226 
   Size:         62     730    1452

  Fragments arranged by size: 

                1452     730     611     359     272     250     220     190
                  93      74      62
 
BspEI T'CCGG_A

Cuts at:   3533    3533 
   Size:       4313
 
BspHI T'CATG_A

Cuts at:   3489    3489 
   Size:       4313
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    524     974    1010    1363    1577    1879    2260     524 
   Size:        450      36     353     214     302     381    2577

  Fragments arranged by size: 

                2577     450     381     353     302     214      36
 
BsrBI CCG'CTC

Cuts at:     50     826    1179    2605    2659    4143    4308      50 
   Size:        776     353    1426      54    1484     165      55

  Fragments arranged by size: 

                1484    1426     776     353     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2225    2225 
   Size:       4313
 
BsrFI r'CCGG_y

Cuts at:   2311    2492    2701    2311 
   Size:        181     209    3923

  Fragments arranged by size: 

                3923     209     181
 
BssHII G'CGCG_C

Cuts at:    203     402    1093     203 
   Size:        199     691    3423

  Fragments arranged by size: 

                3423     691     199
 
BssSI C'ACGA_G

Cuts at:   2584    2942    2584 
   Size:        358    3955
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1690    1690 
   Size:       4313
 
BstBI TT'CG_AA

Cuts at:   2674    2674 
   Size:       4313
 
BstYI r'GATC_y

Cuts at:      1     440     848    1338    1805    1828    2163    2409    3421 
   Size:        439     408     490     467      23     335     246    1012

Cuts at:   3421    3507    3519    3865       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     490     467     449     439     408     346     335
                 246      86      23      12
 
BtgI C'CryG_G

Cuts at:    234     234 
   Size:       4313
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1172    2263    2444    2607    1172 
   Size:       1091     181     163    2878

  Fragments arranged by size: 

                2878    1091     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1556    1556 
   Size:       4313
 
DraI TTT'AAA

Cuts at:   1312    1312 
   Size:       4313
 
DrdI GACnn_nn'nnGTC

Cuts at:   2019    2877    2019 
   Size:        858    3455
 
EaeI y'GGCC_r

Cuts at:    137     231     416     692     884     957    1898    2072    2463 
   Size:         94     185     276     192      73     941     174     391

Cuts at:   2463    2490    3835     137 
   Size:         27    1345     615

  Fragments arranged by size: 

                1345     941     615     391     276     192     185     174
                  94      73      27
 
EagI C'GGCC_G

Cuts at:    231    1898     231 
   Size:       1667    2646
 
EarI CTCTTCn'nnn_

Cuts at:    728    2336    2546     728 
   Size:       1608     210    2495

  Fragments arranged by size: 

                2495    1608     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    243    2521    2831    2977    4123     243 
   Size:       2278     310     146    1146     433

  Fragments arranged by size: 

                2278    1146     433     310     146
 
EcoICRI GAG'CTC

Cuts at:   1325    1325 
   Size:       4313
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    430     537     727     748     769     929     953     958    1000 
   Size:        107     190      21      21     160      24       5      42

Cuts at:   1000    1246    2138    2570    3317    3585     430 
   Size:        246     892     432     747     268    1158

  Fragments arranged by size: 

                1158     892     747     432     268     246     190     160
                 107      42      24      21      21       5
 
EcoNI CCTnn'n_nnAGG

Cuts at:    394    1258     394 
   Size:        864    3449
 
EcoO109I rG'GnC_Cy

Cuts at:    284     466     473     513    1302    1452     284 
   Size:        182       7      40     789     150    3145

  Fragments arranged by size: 

                3145     789     182     150      40       7
 
EcoRI G'AATT_C

Cuts at:   1317    1317 
   Size:       4313
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2332    2332 
   Size:       4313
 
FspI TGC'GCA

Cuts at:    634     667    2094     634 
   Size:         33    1427    2853

  Fragments arranged by size: 

                2853    1427      33
 
HaeII r_GCGC'y

Cuts at:   1129    1747    1995    3017    3996    1129 
   Size:        618     248    1022     979    1446

  Fragments arranged by size: 

                1446    1022     979     618     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    788     820    1024    1056    1201    1233    2155    2185    2187 
   Size:         32     204      32     145      32     922      30       2

Cuts at:   2187    2217     788 
   Size:         30    2884

  Fragments arranged by size: 

                2884     922     204     145      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   1352    1352 
   Size:       4313
 
KasI G'GCGC_C

Cuts at:   1743    1991    1743 
   Size:        248    4065
 
KpnI G_GTAC'C

Cuts at:    456    1333     456 
   Size:        877    3436
 
MluI A'CGCG_T

Cuts at:   3746    3746 
   Size:       4313
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    347    2984    3168     347 
   Size:       2637     184    1492

  Fragments arranged by size: 

                2637    1492     184
 
MscI TGG'CCA

Cuts at:    694     959    2074     694 
   Size:        265    1115    2933

  Fragments arranged by size: 

                2933    1115     265
 
MslI CAynn'nnrTG

Cuts at:    640    2302    2429     640 
   Size:       1662     127    2524

  Fragments arranged by size: 

                2524    1662     127
 
MspA1I CmG'CkG

Cuts at:    236    1047    1110    1136    1152    1200    1424    1738    2098 
   Size:        811      63      26      16      48     224     314     360

Cuts at:   2098    3111    3356     236 
   Size:       1013     245    1193

  Fragments arranged by size: 

                1193    1013     811     360     314     245     224      63
                  48      26      16
 
NaeI GCC'GGC

Cuts at:   2494    2494 
   Size:       4313
 
NarI GG'CG_CC

Cuts at:   1744    1992    1744 
   Size:        248    4065
 
NgoMIV G'CCGG_C

Cuts at:   2492    2492 
   Size:       4313
 
NotI GC'GGCC_GC

Cuts at:    231     231 
   Size:       4313
 
NspI r_CATG'y

Cuts at:    864    1366    1866    2773     864 
   Size:        502     500     907    2404

  Fragments arranged by size: 

                2404     907     502     500
 
PciI A'CATG_T

Cuts at:   2769    2769 
   Size:       4313
 
PfoI T'CCnGG_A

Cuts at:    509     553     509 
   Size:         44    4269
 
PmeI GTTT'AAAC

Cuts at:   1312    1312 
   Size:       4313
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3452    3484    3452 
   Size:         32    4281
 
PpuMI rG'GwC_Cy

Cuts at:    513    1302     513 
   Size:        789    3524
 
PspOMI G'GGCC_C

Cuts at:    472     472 
   Size:       4313
 
PspXI vC'TCGA_Gb

Cuts at:   1258    1258 
   Size:       4313
 
PstI C_TGCA'G

Cuts at:    569     594    1040    1360    2045     569 
   Size:         25     446     320     685    2837

  Fragments arranged by size: 

                2837     685     446     320      25
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4313
 
PvuII CAG'CTG

Cuts at:   1047    1110    1152    1738    2098    1047 
   Size:         63      42     586     360    3262

  Fragments arranged by size: 

                3262     586     360      63      42
 
RsrII CG'GwC_CG

Cuts at:   2508    2508 
   Size:       4313
 
SacI G_AGCT'C

Cuts at:   1327    1327 
   Size:       4313
 
SacII CC_GC'GG

Cuts at:    237     237 
   Size:       4313
 
SalI G'TCGA_C

Cuts at:   1350    1350 
   Size:       4313
 
SapI GCTCTTCn'nnn_

Cuts at:    728    2336    2546     728 
   Size:       1608     210    2495

  Fragments arranged by size: 

                2495    1608     210
 
SbfI CC_TGCA'GG

Cuts at:   1360    1360 
   Size:       4313
 
ScaI AGT'ACT

Cuts at:   3830    3830 
   Size:       4313
 
SfcI C'TryA_G

Cuts at:     33     117     486     565     590    1036    1356    2041    3034 
   Size:         84     369      79      25     446     320     685     993

Cuts at:   3034    3225      33 
   Size:        191    1121

  Fragments arranged by size: 

                1121     993     685     446     369     320     191      84
                  79      25
 
SfoI GGC'GCC

Cuts at:   1745    1993    1745 
   Size:        248    4065
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4313
 
SmaI CCC'GGG

Cuts at:   1335    1335 
   Size:       4313
 
SmlI C'TyrA_G

Cuts at:   1024    1258    1468    2383    2875    3137    1024 
   Size:        234     210     915     492     262    2200

  Fragments arranged by size: 

                2200     915     492     262     234     210
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4313
 
SphI G_CATG'C

Cuts at:   1366    1866    1366 
   Size:        500    3813
 
StuI AGG'CCT

Cuts at:    158     725     158 
   Size:        567    3746
 
StyI C'CwwG_G

Cuts at:   1064    1298    1447    1801    1064 
   Size:        234     149     354    3576

  Fragments arranged by size: 

                3576     354     234     149
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    715    1896    2029    2488    2697     715 
   Size:       1181     133     459     209    2331

  Fragments arranged by size: 

                2331    1181     459     209     133
 
TatI w'GTAC_w

Cuts at:   3828    3828 
   Size:       4313
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    208     240    3830    3862     208 
   Size:         32    3590      32     659

  Fragments arranged by size: 

                3590     659      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    318    2110     318 
   Size:       1792    2521
 
XbaI T'CTAG_A

Cuts at:   1344    1344 
   Size:       4313
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    702    1808     702 
   Size:       1106    3207
 
XhoI C'TCGA_G

Cuts at:   1258    1258 
   Size:       4313
 
XmaI C'CCGG_G

Cuts at:   1333    1333 
   Size:       4313
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NotI
     NspI     PciI     PfoI     PmeI     PpiI    PpuMI   PspOMI    PspXI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AleI     AloI     AscI    AvrII
     BaeI    BfrBI     BmtI     BsgI    BsiWI    BsrGI   BstEII    BstXI
  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NcoI     NdeI     NheI     NruI     NsiI
     PacI     PasI    PflMI     PmlI    PshAI     PsiI     PsrI    SanDI
    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI     XmnI
     ZraI
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