Restriction map for pF1KB6561
 (Linear) (Six-base) MAPSORT of: KIBB6561.seq  Check: 1692  from: 1  to: 1122



KIBB6561.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

AflII C'TTAA_G

Cuts at:      0     808    1122 
   Size:        808     314
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     721    1122 
   Size:        721     401
 
AlwNI CAG_nnn'CTG

Cuts at:      0     308    1122 
   Size:        308     814
 
ApaLI G'TGCA_C

Cuts at:      0    1093    1122 
   Size:       1093      29
 
ApoI r'AATT_y

Cuts at:      0     447    1122 
   Size:        447     675
 
BanI G'GyrC_C

Cuts at:      0     251     490    1122 
   Size:        251     239     632

  Fragments arranged by size: 

                 632     251     239
 
BanII G_rGCy'C

Cuts at:      0    1109    1122 
   Size:       1109      13
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     330     764    1034    1122 
   Size:        330     434     270      88

  Fragments arranged by size: 

                 434     330     270      88
 
BclI T'GATC_A

Cuts at:      0      68    1122 
   Size:         68    1054
 
BfrBI ATG'CAT

Cuts at:      0     586    1122 
   Size:        586     536
 
Bme1580I G_kGCm'C

Cuts at:      0    1097    1122 
   Size:       1097      25
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     312     557     628     832    1122 
   Size:        312     245      71     204     290

  Fragments arranged by size: 

                 312     290     245     204      71
 
Bpu10I CC'TnA_GC

Cuts at:      0     504     628    1122 
   Size:        504     124     494

  Fragments arranged by size: 

                 504     494     124
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     384    1122 
   Size:        384     738
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     268     330    1122 
   Size:        268      62     792

  Fragments arranged by size: 

                 792     268      62
 
BsaAI yAC'GTr

Cuts at:      0     940    1122 
   Size:        940     182
 
BsaWI w'CCGG_w

Cuts at:      0    1097    1122 
   Size:       1097      25
 
BseYI C'CCAG_C

Cuts at:      0     202    1122 
   Size:        202     920
 
BsiHKAI G_wGCw'C

Cuts at:      0     137    1097    1122 
   Size:        137     960      25

  Fragments arranged by size: 

                 960     137      25
 
BsmI GAATG_Cn'

Cuts at:      0     588    1122 
   Size:        588     534
 
Bsp1286I G_dGCh'C

Cuts at:      0     137    1097    1109    1122 
   Size:        137     960      12      13

  Fragments arranged by size: 

                 960     137      13      12
 
BspHI T'CATG_A

Cuts at:      0     996    1122 
   Size:        996     126
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     312    1122 
   Size:        312     810
 
BsrDI GCAATG_nn'

Cuts at:      0     498    1122 
   Size:        498     624
 
BsrGI T'GTAC_A

Cuts at:      0     395    1122 
   Size:        395     727
 
BstEII G'GTnAC_C

Cuts at:      0     621    1122 
   Size:        621     501
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     145     291    1122 
   Size:        145     146     831

  Fragments arranged by size: 

                 831     146     145
 
BtgI C'CryG_G

Cuts at:      0      85    1122 
   Size:         85    1037
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     505    1122 
   Size:        505     617
 
BtsI GCAGTG_nn'

Cuts at:      0      71     899    1122 
   Size:         71     828     223

  Fragments arranged by size: 

                 828     223      71
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     219     254    1122 
   Size:        219      35     868

  Fragments arranged by size: 

                 868     219      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     747    1040    1122 
   Size:        747     293      82

  Fragments arranged by size: 

                 747     293      82
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     312     557     628     832    1122 
   Size:        312     245      71     204     290

  Fragments arranged by size: 

                 312     290     245     204      71
 
EcoRI G'AATT_C

Cuts at:      0     447    1122 
   Size:        447     675
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     666    1122 
   Size:        666     456
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     806    1122 
   Size:        806     316
 
MspA1I CmG'CkG

Cuts at:      0     291    1080    1122 
   Size:        291     789      42

  Fragments arranged by size: 

                 789     291      42
 
NcoI C'CATG_G

Cuts at:      0      85    1122 
   Size:         85    1037
 
NsiI A_TGCA'T

Cuts at:      0     588    1122 
   Size:        588     534
 
PasI CC'CwG_GG

Cuts at:      0    1110    1122 
   Size:       1110      12
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      40    1122 
   Size:         40    1082
 
PfoI T'CCnGG_A

Cuts at:      0     605    1122 
   Size:        605     517
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     709     741    1122 
   Size:        709      32     381

  Fragments arranged by size: 

                 709     381      32
 
PstI C_TGCA'G

Cuts at:      0     323    1085    1122 
   Size:        323     762      37

  Fragments arranged by size: 

                 762     323      37
 
PvuII CAG'CTG

Cuts at:      0     291    1080    1122 
   Size:        291     789      42

  Fragments arranged by size: 

                 789     291      42
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1040    1122 
   Size:       1040      82
 
SfcI C'TryA_G

Cuts at:      0     207     319    1081    1122 
   Size:        207     112     762      41

  Fragments arranged by size: 

                 762     207     112      41
 
SmlI C'TyrA_G

Cuts at:      0     363     808    1122 
   Size:        363     445     314

  Fragments arranged by size: 

                 445     363     314
 
SnaBI TAC'GTA

Cuts at:      0     940    1122 
   Size:        940     182
 
StuI AGG'CCT

Cuts at:      0     660    1122 
   Size:        660     462
 
StyI C'CwwG_G

Cuts at:      0      85    1122 
   Size:         85    1037
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     918    1122 
   Size:        918     204
 
TatI w'GTAC_w

Cuts at:      0     395    1122 
   Size:        395     727
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     863    1122 
   Size:        863     259
 

 Enzymes that do cut:

    AflII     AlfI    AlwNI    ApaLI     ApoI     BanI    BanII     BbsI
     BclI    BfrBI Bme1580I     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaWI    BseYI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI
    BsrGI   BstEII    BstXI     BtgI    BtgZI     BtsI    CspCI     EarI
  Eco57MI    EcoRI     FalI     MmeI   MspA1I     NcoI     NsiI     PasI
    PflMI     PfoI     PpiI     PstI    PvuII     SapI     SfcI     SmlI
    SnaBI     StuI     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AloI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI    BsaBI
    BsaHI    BsaXI    BseRI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI    BstYI  BstZ17I
   Bsu36I     ClaI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     MslI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NspI     PacI     PciI     PmeI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SpeI     SphI     SrfI     SspI     SwaI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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