Restriction map for pF1KB6676
 (Linear) (Six-base) MAPSORT of: KIBB6676.seq  Check: 9917  from: 1  to: 1362



KIBB6676.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     374    1362 
   Size:        374     988
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1111    1362 
   Size:       1111     251
 
AlwNI CAG_nnn'CTG

Cuts at:      0      15      78    1362 
   Size:         15      63    1284

  Fragments arranged by size: 

                1284      63      15
 
ApaI G_GGCC'C

Cuts at:      0     306     792    1362 
   Size:        306     486     570

  Fragments arranged by size: 

                 570     486     306
 
ApoI r'AATT_y

Cuts at:      0     358    1049    1362 
   Size:        358     691     313

  Fragments arranged by size: 

                 691     358     313
 
AvaI C'yCGr_G

Cuts at:      0     784    1362 
   Size:        784     578
 
BanII G_rGCy'C

Cuts at:      0      56     306     338     792    1304    1362 
   Size:         56     250      32     454     512      58

  Fragments arranged by size: 

                 512     454     250      58      56      32
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     689     770    1362 
   Size:        689      81     592

  Fragments arranged by size: 

                 689     592      81
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1177    1211    1362 
   Size:       1177      34     151

  Fragments arranged by size: 

                1177     151      34
 
BfrBI ATG'CAT

Cuts at:      0     583    1362 
   Size:        583     779
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1246    1362 
   Size:       1246     116
 
BlpI GC'TnA_GC

Cuts at:      0      55    1362 
   Size:         55    1307
 
Bme1580I G_kGCm'C

Cuts at:      0     306     792    1362 
   Size:        306     486     570

  Fragments arranged by size: 

                 570     486     306
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     484     504    1362 
   Size:        484      20     858

  Fragments arranged by size: 

                 858     484      20
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1045    1362 
   Size:       1045     317
 
BsaBI GATnn'nnATC

Cuts at:      0      38    1362 
   Size:         38    1324
 
BsaHI Gr'CG_yC

Cuts at:      0    1202    1362 
   Size:       1202     160
 
BsaWI w'CCGG_w

Cuts at:      0     408     968    1362 
   Size:        408     560     394

  Fragments arranged by size: 

                 560     408     394
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     997    1362 
   Size:        997     365
 
BsiHKAI G_wGCw'C

Cuts at:      0      56     278    1090    1304    1362 
   Size:         56     222     812     214      58

  Fragments arranged by size: 

                 812     222     214      58      56
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     729    1362 
   Size:        729     633
 
Bsp1286I G_dGCh'C

Cuts at:      0      56     278     306     338     792    1090    1304    1362 
   Size:         56     222      28      32     454     298     214      58

  Fragments arranged by size: 

                 454     298     222     214      58      56      32      28
            
 
BspEI T'CCGG_A

Cuts at:      0     408    1362 
   Size:        408     954
 
BsrBI CCG'CTC

Cuts at:      0    1003    1362 
   Size:       1003     359
 
BsrGI T'GTAC_A

Cuts at:      0      26    1362 
   Size:         26    1336
 
BssSI C'ACGA_G

Cuts at:      0     758    1362 
   Size:        758     604
 
BstYI r'GATC_y

Cuts at:      0      33      74     104    1362 
   Size:         33      41      30    1258

  Fragments arranged by size: 

                1258      41      33      30
 
BtgI C'CryG_G

Cuts at:      0    1044    1362 
   Size:       1044     318
 
ClaI AT'CG_AT

Cuts at:      0    1187    1362 
   Size:       1187     175
 
DraI TTT'AAA

Cuts at:      0     978    1362 
   Size:        978     384
 
EarI CTCTTCn'nnn_

Cuts at:      0     326     494     641     727     795    1362 
   Size:        326     168     147      86      68     567

  Fragments arranged by size: 

                 567     326     168     147      86      68
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1264    1362 
   Size:       1264      98
 
EcoICRI GAG'CTC

Cuts at:      0      54    1302    1362 
   Size:         54    1248      60

  Fragments arranged by size: 

                1248      60      54
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     374     484     504    1362 
   Size:        374     110      20     858

  Fragments arranged by size: 

                 858     374     110      20
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1294    1362 
   Size:       1294      68
 
EcoO109I rG'GnC_Cy

Cuts at:      0     302     788    1362 
   Size:        302     486     574

  Fragments arranged by size: 

                 574     486     302
 
EcoRI G'AATT_C

Cuts at:      0     358    1049    1362 
   Size:        358     691     313

  Fragments arranged by size: 

                 691     358     313
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     457     489    1362 
   Size:        457      32     873

  Fragments arranged by size: 

                 873     457      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     132    1139    1362 
   Size:        132    1007     223

  Fragments arranged by size: 

                1007     223     132
 
MslI CAynn'nnrTG

Cuts at:      0    1093    1362 
   Size:       1093     269
 
MspA1I CmG'CkG

Cuts at:      0     724    1335    1362 
   Size:        724     611      27

  Fragments arranged by size: 

                 724     611      27
 
NcoI C'CATG_G

Cuts at:      0    1044    1362 
   Size:       1044     318
 
NdeI CA'TA_TG

Cuts at:      0     579    1362 
   Size:        579     783
 
NsiI A_TGCA'T

Cuts at:      0     585    1362 
   Size:        585     777
 
NspI r_CATG'y

Cuts at:      0    1098    1240    1362 
   Size:       1098     142     122

  Fragments arranged by size: 

                1098     142     122
 
PasI CC'CwG_GG

Cuts at:      0    1290    1362 
   Size:       1290      72
 
PspOMI G'GGCC_C

Cuts at:      0     302     788    1362 
   Size:        302     486     574

  Fragments arranged by size: 

                 574     486     302
 
PstI C_TGCA'G

Cuts at:      0     404    1200    1362 
   Size:        404     796     162

  Fragments arranged by size: 

                 796     404     162
 
PvuII CAG'CTG

Cuts at:      0     724    1335    1362 
   Size:        724     611      27

  Fragments arranged by size: 

                 724     611      27
 
SacI G_AGCT'C

Cuts at:      0      56    1304    1362 
   Size:         56    1248      58

  Fragments arranged by size: 

                1248      58      56
 
SapI GCTCTTCn'nnn_

Cuts at:      0     326    1362 
   Size:        326    1036
 
SfcI C'TryA_G

Cuts at:      0     400    1196    1206    1231    1362 
   Size:        400     796      10      25     131

  Fragments arranged by size: 

                 796     400     131      25      10
 
SmlI C'TyrA_G

Cuts at:      0    1060    1362 
   Size:       1060     302
 
SphI G_CATG'C

Cuts at:      0    1098    1240    1362 
   Size:       1098     142     122

  Fragments arranged by size: 

                1098     142     122
 
SspI AAT'ATT

Cuts at:      0     537    1362 
   Size:        537     825
 
StyI C'CwwG_G

Cuts at:      0     743    1044    1244    1362 
   Size:        743     301     200     118

  Fragments arranged by size: 

                 743     301     200     118
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     225     882    1362 
   Size:        225     657     480

  Fragments arranged by size: 

                 657     480     225
 
TatI w'GTAC_w

Cuts at:      0      26    1362 
   Size:         26    1336
 
Tth111I GACn'n_nGTC

Cuts at:      0     939    1362 
   Size:        939     423
 
XbaI T'CTAG_A

Cuts at:      0     255    1362 
   Size:        255    1107
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1287    1362 
   Size:       1287      75
 
XmnI GAAnn'nnTTC

Cuts at:      0    1079    1362 
   Size:       1079     283
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI     ApaI     ApoI     AvaI    BanII     BbsI
     BcgI    BfrBI     BglI     BlpI Bme1580I     BpmI    BpuEI    BsaBI
    BsaHI    BsaWI    BseRI  BsiHKAI    BsmBI Bsp1286I    BspEI    BsrBI
    BsrGI    BssSI    BstYI     BtgI     ClaI     DraI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    Hin4I     MmeI     MslI
   MspA1I     NcoI     NdeI     NsiI     NspI     PasI   PspOMI     PstI
    PvuII     SacI     SapI     SfcI     SmlI     SphI     SspI     StyI
    TaqII     TatI  Tth111I     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI    BciVI     BclI
    BglII    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI
    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BspHI    BspMI
    BsrDI    BsrFI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     BtsI    CspCI   DraIII     DrdI     EaeI     EagI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI
   NgoMIV     NheI     NotI     NruI     PacI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     StuI     SwaI     TstI     XhoI     XmaI     ZraI
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