Restriction map for pF1KB6672
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp700I     Asp718I     AspEI       AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BclI        
BglI        BlnI        BlpI        Bpu1102I    BsaHI       
BsiEI       BsiWI       BsmBI       BsoBI       Bsp1407I    
BspDI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     FauI        FbaI        FseI        FspI        
HaeII       HhaI        Hin1I       HinP1I      HpaI        
KasI        KpnI        KspI        MfeI        MluI        
MluNI       MscI        MunI        MvnI        NaeI        
NarI        NcoI        NgoMIV      NheI        NotI        
NruI        NspI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
Psp1406I    PstI        PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccIII        593  TCCGGA               T^CCGGA
AciI          975  CCGC                 CCGC(-3/-1)
AflII          22  CTTAAG               C^TTAAG
AseI          781  ATTAAT               AT^TAAT
AsnI          781  ATTAAT               AT^TAAT
AspHI        1256  GTGCTC               G[AT]GC[AT]^C
AspI          463  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BcnI         1086  CCGGG                CC^[GC]GG
BfrI           22  CTTAAG               C^TTAAG
BglII         690  AGATCT               A^GATCT
BmyI         1256  GTGCTC               G[AGT]GC[ACT]^C
BsaAI        1237  CACGTA               [CT]AC^GT[GA]
BsaBI         685  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          322  GAGACC               GGTCTC(1/5)
BsaJI         311  CCTTGG               C^C[ACGT][ACGT]GG
BseAI         593  TCCGGA               T^CCGGA
BsiHKAI      1256  GTGCTC               G[AT]GC[AT]^C
BsiYI        1086  CCGGGATCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI         1086  CCGGGATCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         876  GGGAC                GGGAC(10/14)
BsmI         1182  GAATGC               GAATGC(1/-1)
Bsp1286I     1256  GTGCTC               G[AGT]GC[ACT]^C
BspEI         593  TCCGGA               T^CCGGA
BspHI         470  TCATGA               T^CATGA
BstNI         208  CCTGG                CC^[AT]GG
Cac8I         427  GCCAGC               GC[ACGT]^[ACGT]GC
DraII         876  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO109I      876  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI         589  GAATTC               G^AATTC
EcoRII        208  CCTGG                ^CC[AT]GG
EcoRV         751  GATATC               GAT^ATC
EcoT14I       311  CCTTGG               C^C[AT][AT]GG
EcoT22I         1  ATGCAT               ATGCA^T
HincII        481  GTCAAC               GT[CT]^[GA]AC
HindII        481  GTCAAC               GT[CT]^[GA]AC
HindIII       722  AAGCTT               A^AGCTT
MaeII        1238  ACGT                 A^CGT
MamI          685  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          593  TCCGGA               T^CCGGA
MspA1I        584  CAGCTG               C[AC]G^C[GT]G
MvaI          208  CCTGG                CC^[AT]GG
NciI         1086  CCGGG                CC^[GC]GG
NdeI         1177  CATATG               CA^TATG
NsiI            1  ATGCAT               ATGCA^T
PflFI         463  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         876  GGGACCT              [GA]G^G[AT]CC[CT]
PshAI         878  GACCTCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         781  ATTAAT               AT^TAAT
PvuII         584  CAGCTG               CAG^CTG
RcaI          470  TCATGA               T^CATGA
ScaI          293  AGTACT               AGT^ACT
SfcI         1199  CTATAG               C^T[GA][CT]AG
SmlI           22  CTTAAG               C^T[CT][GA]AG
StyI          311  CCTTGG               C^C[AT][AT]GG
Tth111I       463  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          555  CCAAATTCCTCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwNI         581  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1207  CAGACTCTG           
AvaII         606  GGACC                G^G[AT]CC
              877  GGACC               
BbsI          122  GAAGAC               GAAGAC(2/6)
              869  GAAGAC              
BfaI           61  CTAG                 C^TAG
              424  CTAG                
BpuAI         122  GAAGAC               GAAGAC(2/6)
              869  GAAGAC              
BsaWI         593  TCCGGA               [AT]^CCGG[AT]
              608  ACCGGA              
BsgI          227  CTGCAC               GTGCAG(16/14)
              815  GTGCAG              
BssKI         208  CCTGG                ^CC[ACGT]GG
             1086  CCGGG               
EarI          283  GAAGAG               CTCTTC(1/4)
              319  GAAGAG              
HaeIII        717  GGCC                 GG^CC
              906  GGCC                
HgaI          833  GACGC                GACGC(5/10)
              220  GCGTC               
Ksp632I       283  GAAGAG               CTCTTC(1/4)
              319  GAAGAG              
MaeI           61  CTAG                 C^TAG
              424  CTAG                
MaeIII        577  GTGAC                ^GT[ACGT]AC
              760  GTGAC               
MslI          465  CATGGTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1177  CATATGAATG          
NlaIV         131  GGAACC               GG[ACGT]^[ACGT]CC
              876  GGGACC              
ScrFI         208  CCTGG                CC^[ACGT]GG
             1086  CCGGG               
TaqI          540  TCGA                 T^CGA
              936  TCGA                
Tsp45I        577  GTGAC                ^GT[GC]AC
              760  GTGAC               
TthHB8I       540  TCGA                 T^CGA
              936  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
BbvI        BciVI       BpmI        BseRI       BsmAI       
BsrI        BstF5I      BstYI       Cfr13I      DdeI        
DpnII       Fnu4HI      FokI        HapII       HinfI       
HpaII       HphI        ItaI        MboI        MboII       
MflI        MnlI        MseI        MspI        MwoI        
NdeII       NlaIII      PleI        RsaI        Sau3AI      
Sau96I      SfaNI       Tru9I       TseI        Tsp509I     
TspRI       XhoII       
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