Restriction map for pF1KB6660
 (Linear) (Six-base) MAPSORT of: KIBB6660.seq  Check: 3882  from: 1  to: 1353



KIBB6660.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      82    1353 
   Size:         82    1271
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212    1353 
   Size:        212    1141
 
AhdI GACnn_n'nnGTC

Cuts at:      0     205     927    1353 
   Size:        205     722     426

  Fragments arranged by size: 

                 722     426     205
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     584    1353 
   Size:        584     769
 
ApaI G_GGCC'C

Cuts at:      0     472    1353 
   Size:        472     881
 
ApoI r'AATT_y

Cuts at:      0     349    1069    1177    1345    1353 
   Size:        349     720     108     168       8

  Fragments arranged by size: 

                 720     349     168     108       8
 
AseI AT'TA_AT

Cuts at:      0     259    1353 
   Size:        259    1094
 
BanII G_rGCy'C

Cuts at:      0     472    1353 
   Size:        472     881
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     239     295     926    1353 
   Size:        239      56     631     427

  Fragments arranged by size: 

                 631     427     239      56
 
BfrBI ATG'CAT

Cuts at:      0     136     798    1353 
   Size:        136     662     555

  Fragments arranged by size: 

                 662     555     136
 
Bme1580I G_kGCm'C

Cuts at:      0     472    1353 
   Size:        472     881
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1204    1353 
   Size:       1204     149
 
Bpu10I CC'TnA_GC

Cuts at:      0     946    1353 
   Size:        946     407
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     811    1353 
   Size:        212     599     542

  Fragments arranged by size: 

                 599     542     212
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     811    1353 
   Size:        811     542
 
BseYI C'CCAG_C

Cuts at:      0     465    1100    1353 
   Size:        465     635     253

  Fragments arranged by size: 

                 635     465     253
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     449    1353 
   Size:        449     904
 
Bsp1286I G_dGCh'C

Cuts at:      0     472    1353 
   Size:        472     881
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      82    1353 
   Size:         82    1271
 
BsrDI GCAATG_nn'

Cuts at:      0     156    1353 
   Size:        156    1197
 
BstBI TT'CG_AA

Cuts at:      0    1185    1259    1353 
   Size:       1185      74      94

  Fragments arranged by size: 

                1185      94      74
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     163    1353 
   Size:        163    1190
 
Bsu36I CC'TnA_GG

Cuts at:      0    1027    1353 
   Size:       1027     326
 
BtgI C'CryG_G

Cuts at:      0      33    1353 
   Size:         33    1320
 
BtsI GCAGTG_nn'

Cuts at:      0      48     168    1040    1353 
   Size:         48     120     872     313

  Fragments arranged by size: 

                 872     313     120      48
 
EaeI y'GGCC_r

Cuts at:      0      96     887    1353 
   Size:         96     791     466

  Fragments arranged by size: 

                 791     466      96
 
EarI CTCTTCn'nnn_

Cuts at:      0     482     737    1353 
   Size:        482     255     616

  Fragments arranged by size: 

                 616     482     255
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212    1353 
   Size:        212    1141
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     214    1353 
   Size:        214    1139
 
EcoO109I rG'GnC_Cy

Cuts at:      0     469     950    1353 
   Size:        469     481     403

  Fragments arranged by size: 

                 481     469     403
 
EcoRI G'AATT_C

Cuts at:      0    1177    1345    1353 
   Size:       1177     168       8

  Fragments arranged by size: 

                1177     168       8
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     322    1353 
   Size:        322    1031
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     191     223     913     945    1353 
   Size:        191      32     690      32     408

  Fragments arranged by size: 

                 690     408     191      32      32
 
HindIII A'AGCT_T

Cuts at:      0     787     808    1353 
   Size:        787      21     545

  Fragments arranged by size: 

                 787     545      21
 
MfeI C'AATT_G

Cuts at:      0     140    1353 
   Size:        140    1213
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1078    1320    1353 
   Size:       1078     242      33

  Fragments arranged by size: 

                1078     242      33
 
MscI TGG'CCA

Cuts at:      0      98     889    1353 
   Size:         98     791     464

  Fragments arranged by size: 

                 791     464      98
 
MslI CAynn'nnrTG

Cuts at:      0     107     161    1118    1353 
   Size:        107      54     957     235

  Fragments arranged by size: 

                 957     235     107      54
 
MspA1I CmG'CkG

Cuts at:      0      35     433     465    1005    1353 
   Size:         35     398      32     540     348

  Fragments arranged by size: 

                 540     398     348      35      32
 
NdeI CA'TA_TG

Cuts at:      0     760    1353 
   Size:        760     593
 
NsiI A_TGCA'T

Cuts at:      0     138     800    1353 
   Size:        138     662     553

  Fragments arranged by size: 

                 662     553     138
 
NspI r_CATG'y

Cuts at:      0    1089    1353 
   Size:       1089     264
 
PspOMI G'GGCC_C

Cuts at:      0     468    1353 
   Size:        468     885
 
PstI C_TGCA'G

Cuts at:      0     438    1284    1353 
   Size:        438     846      69

  Fragments arranged by size: 

                 846     438      69
 
PvuII CAG'CTG

Cuts at:      0     433     465    1353 
   Size:        433      32     888

  Fragments arranged by size: 

                 888     433      32
 
SacII CC_GC'GG

Cuts at:      0      36    1353 
   Size:         36    1317
 
SfcI C'TryA_G

Cuts at:      0     434    1280    1353 
   Size:        434     846      73

  Fragments arranged by size: 

                 846     434      73
 
SmlI C'TyrA_G

Cuts at:      0     227     790    1353 
   Size:        227     563     563

  Fragments arranged by size: 

                 563     563     227
 
StyI C'CwwG_G

Cuts at:      0     953    1353 
   Size:        953     400
 
TatI w'GTAC_w

Cuts at:      0     242    1353 
   Size:        242    1111
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1196    1228    1353 
   Size:       1196      32     125

  Fragments arranged by size: 

                1196     125      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     353    1181    1353 
   Size:        353     828     172

  Fragments arranged by size: 

                 828     353     172
 

 Enzymes that do cut:

     AarI     AcuI     AhdI     AlfI     ApaI     ApoI     AseI    BanII
     BbsI    BfrBI Bme1580I     BmrI   Bpu10I    BpuEI    BseRI    BseYI
     BsgI Bsp1286I    BspMI    BsrDI    BstBI    BstXI   Bsu36I     BtgI
     BtsI     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
    Hin4I  HindIII     MfeI     MmeI     MscI     MslI   MspA1I     NdeI
     NsiI     NspI   PspOMI     PstI    PvuII    SacII     SfcI     SmlI
     StyI     TatI     TstI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AloI    AlwNI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI     BpmI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI   BstEII    BstYI  BstZ17I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoRV     FseI
     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MluI
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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