Restriction map for pF1KB6650
##################################
# NO CUT ENZYMES (  135 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbvCI       BcgI        BfrI        BglI        
BlnI        BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HaeII       HgaI        HpaI        
KasI        KpnI        KspI        MluI        MluNI       
MroI        MscI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspV        PacI        
PflFI       PinAI       PleI        PmeI        PpuMI       
PshAI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
XbaI        XcmI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

AatII         109  GACGTC               GACGT^C
AciI         1171  CCGC                 CCGC(-3/-1)
AcyI          109  GACGTC               G[GA]^CG[CT]C
AfaI         1078  GTAC                 GT^AC
AlwNI         469  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI          906  ATTAAT               AT^TAAT
AsnI          906  ATTAAT               AT^TAAT
AspHI         526  GAGCTC               G[AT]GC[AT]^C
AvaI          844  CTCGAG               C^[CT]CG[GA]G
BanII         526  GAGCTC               G[GA]GC[CT]^C
BbrPI        1318  CACGTG               CAC^GTG
BciVI        1145  GTATCC               GTATCC(6/5)
BclI         1218  TGATCA               T^GATCA
BcnI          348  CCGGG                CC^[GC]GG
BfaI          550  CTAG                 C^TAG
BlpI          523  GCTGAGC              GC^T[ACGT]AGC
BmyI          526  GAGCTC               G[AGT]GC[ACT]^C
Bpu1102I      523  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1318  CACGTG               [CT]AC^GT[GA]
BsaHI         109  GACGTC               G[GA]^CG[CT]C
BsaI            7  GAGACC               GGTCTC(1/5)
BsiHKAI       526  GAGCTC               G[AT]GC[AT]^C
BsmFI         107  GGGAC                GGGAC(10/14)
BsoBI         844  CTCGAG               C^[CT]CG[GA]G
Bsp1286I      526  GAGCTC               G[AGT]GC[ACT]^C
BspLU11I      115  ACATGT               A^CATGT
BspMI         290  ACCTGC               ACCTGC(4/8)
BsrBI        1171  CCGCTC               CCGCTC(-3/-3)
BsrDI         886  GCAATG               GCAATG(2/0)
BsrI          315  CCAGT                ACTGG(1/-1)
CelII         523  GCTGAGC              GC^T[ACGT]AGC
CfoI         1100  GCGC                 GCG^C
EcoNI         291  CCTGCCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI         1170  CCCGC                CCCGC(4/6)
FbaI         1218  TGATCA               T^GATCA
HaeIII        702  GGCC                 GG^CC
HapII         348  CCGG                 C^CGG
HhaI         1100  GCGC                 GCG^C
Hin1I         109  GACGTC               G[GA]^CG[CT]C
HinP1I       1100  GCGC                 G^CGC
HincII        711  GTCAAC               GT[CT]^[GA]AC
HindII        711  GTCAAC               GT[CT]^[GA]AC
HinfI         680  GAATC                G^A[ACGT]TC
HpaII         348  CCGG                 C^CGG
HphI          131  TCACC                GGTGA(8/7)
MaeI          550  CTAG                 C^TAG
MfeI         1260  CAATTG               C^AATTG
MslI          210  CACCTTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          348  CCGG                 C^CGG
MunI         1260  CAATTG               C^AATTG
NciI          348  CCGGG                CC^[GC]GG
PaeR7I        844  CTCGAG               C^TCGAG
PflMI        1135  CCATATTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1318  CACGTG               CAC^GTG
PmlI         1318  CACGTG               CAC^GTG
PshBI         906  ATTAAT               AT^TAAT
PstI          970  CTGCAG               CTGCA^G
RsaI         1078  GTAC                 GT^AC
SacI          526  GAGCTC               GAGCT^C
TfiI          680  GAATC                G^A[AT]TC
Van91I       1135  CCATATTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          844  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AflIII        115  ACATGT               A^C[GA][CT]GT
             1319  ACGTGT              
AvaII         345  GGTCC                G^G[AT]CC
              831  GGACC               
BglII        1280  AGATCT               A^GATCT
             1304  AGATCT              
BpmI          496  CTCCAG               CTGGAG(16/14)
              101  CTGGAG              
BsaBI         165  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              675  GATGGGAATC          
BseRI           4  GAGGAG               GAGGAG(10/8)
              544  GAGGAG              
Cac8I         519  GCAGGC               GC[ACGT]^[ACGT]GC
             1169  GCCCGC              
EarI          427  GAAGAG               CTCTTC(1/4)
             1014  GAAGAG              
EcoT14I       296  CCAAGG               C^C[AT][AT]GG
             1226  CCTTGG              
HindIII       281  AAGCTT               A^AGCTT
              736  AAGCTT              
Ksp632I       427  GAAGAG               CTCTTC(1/4)
             1014  GAAGAG              
MaeII         110  ACGT                 A^CGT
             1319  ACGT                
MamI          165  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              675  GATGGGAATC          
NspI          115  ACATGT               [GA]CATG^[CT]
              515  ACATGC              
SmlI          716  CTCAAG               C^T[CT][GA]AG
              844  CTCGAG              
StyI          296  CCAAGG               C^C[AT][AT]GG
             1226  CCTTGG              
TaqI          153  TCGA                 T^CGA
              845  TCGA                
Tsp45I        112  GTCAC                ^GT[GC]AC
             1274  GTGAC               
TthHB8I       153  TCGA                 T^CGA
              845  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AluI        AlwI        BbsI        BbvI        BpuAI       
BsaJI       BsiYI       BslI        BsmAI       BssKI       
BstF5I      BstNI       BstYI       Cfr13I      DdeI        
DpnII       EcoRII      Fnu4HI      FokI        ItaI        
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MspA1I      MvaI        MwoI        NdeII       
NlaIII      PvuII       Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        Tru9I       TseI        Tsp509I     
TspRI       XhoII       
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