Restriction map for
pF1KB6631
(Circular) (Six-base) MAPSORT of: pF1KB6631.seq Check: 3414 from: 1 to: 4325
pF1KB6631 4325 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 1363 1363
Size: 4325
Acc65I G'GTAC_C
Cuts at: 1341 1341
Size: 4325
AclI AA'CG_TT
Cuts at: 1157 4165 1157
Size: 3008 1317
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 517 586 655 724 793 862 2150 2582
Size: 69 69 69 69 69 69 1288 432
Cuts at: 2582 3329 3597 448
Size: 747 268 1176
Fragments arranged by size:
1288 1176 747 432 268 69 69 69
69 69 69
AflIII A'CryG_T
Cuts at: 1677 2781 3758 1677
Size: 1104 977 2244
Fragments arranged by size:
2244 1104 977
AgeI A'CCGG_T
Cuts at: 2713 2713
Size: 4325
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1272 1272
Size: 4325
AlwNI CAG_nnn'CTG
Cuts at: 141 348 417 486 555 624 693 762 831
Size: 207 69 69 69 69 69 69 69
Cuts at: 831 1294 1630 3197 141
Size: 463 336 1567 1269
Fragments arranged by size:
1567 1269 463 336 207 69 69 69
69 69 69 69
ApaI G_GGCC'C
Cuts at: 975 975
Size: 4325
ApaLI G'TGCA_C
Cuts at: 3095 3095
Size: 4325
ApoI r'AATT_y
Cuts at: 1180 1225 1329 3678 3934 1180
Size: 45 104 2349 256 1571
Fragments arranged by size:
2349 1571 256 104 45
AseI AT'TA_AT
Cuts at: 20 20
Size: 4325
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4325
AvaI C'yCGr_G
Cuts at: 191 1345 191
Size: 1154 3171
BamHI G'GATC_C
Cuts at: 1350 1350
Size: 4325
BanI G'GyrC_C
Cuts at: 1341 1755 2003 2038 1341
Size: 414 248 35 3628
Fragments arranged by size:
3628 414 248 35
BanII G_rGCy'C
Cuts at: 275 380 975 1151 1339 2369 275
Size: 105 595 176 188 1030 2231
Fragments arranged by size:
2231 1030 595 188 176 105
BbeI G_GCGC'C
Cuts at: 1759 2007 1759
Size: 248 4077
BbsI GAAGACnn'nnnn_
Cuts at: 1019 1175 1019
Size: 156 4169
BbvCI CC'TCA_GC
Cuts at: 998 998
Size: 4325
BciVI GTATCCnnnnn_n'
Cuts at: 190 2223 2984 4325 190
Size: 2033 761 1341 190
Fragments arranged by size:
2033 1341 761 190
BclI T'GATC_A
Cuts at: 1845 1845
Size: 4325
BglII A'GATC_T
Cuts at: 404 473 542 611 680 749 818 1065 1840
Size: 69 69 69 69 69 69 247 775
Cuts at: 1840 3877 404
Size: 2037 852
Fragments arranged by size:
2037 852 775 247 69 69 69 69
69 69
BlpI GC'TnA_GC
Cuts at: 207 1437 207
Size: 1230 3095
Bme1580I G_kGCm'C
Cuts at: 975 1950 2043 3099 975
Size: 975 93 1056 2201
Fragments arranged by size:
2201 1056 975 93
BmrI ACTGGGnnnn_n'
Cuts at: 1537 1618 1953 4097 1537
Size: 81 335 2144 1765
Fragments arranged by size:
2144 1765 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2692 2692
Size: 4325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 1245 1271 172
Size: 1073 26 3226
Fragments arranged by size:
3226 1073 26
Bpu10I CC'TnA_GC
Cuts at: 998 998
Size: 4325
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1501 2380 2872 3170 1501
Size: 879 492 298 2656
Fragments arranged by size:
2656 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4029 4029
Size: 4325
BsaAI yAC'GTr
Cuts at: 1085 1678 1085
Size: 593 3732
BsaBI GATnn'nnATC
Cuts at: 6 1070 1839 3876 6
Size: 1064 769 2037 455
Fragments arranged by size:
2037 1064 769 455
BsaHI Gr'CG_yC
Cuts at: 1756 2004 2706 4202 1756
Size: 248 702 1496 1879
Fragments arranged by size:
1879 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1514 2035 2713 2987 3134 3545 1514
Size: 521 678 274 147 411 2294
Fragments arranged by size:
2294 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 238 1015 1157 169
Size: 69 777 142 3337
Fragments arranged by size:
3337 777 142 69
BseYI C'CCAG_C
Cuts at: 141 268 298 353 422 836 926 974 1117
Size: 127 30 55 69 414 90 48 143
Cuts at: 1117 1750 2508 3085 3581 141
Size: 633 758 577 496 885
Fragments arranged by size:
885 758 633 577 496 414 143 127
90 69 55 48 30
BsiEI CG_ry'CG
Cuts at: 104 1867 1913 3121 104
Size: 1763 46 1208 1308
Fragments arranged by size:
1763 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 380 1339 2117 2307 3099 380
Size: 959 778 190 792 1606
Fragments arranged by size:
1606 959 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 275 380 975 1151 1339 1950 2043 2117 2307
Size: 105 595 176 188 611 93 74 190
Cuts at: 2307 2369 3099 275
Size: 62 730 1501
Fragments arranged by size:
1501 730 611 595 190 188 176 105
93 74 62
BspEI T'CCGG_A
Cuts at: 3545 3545
Size: 4325
BspHI T'CATG_A
Cuts at: 3501 3501
Size: 4325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1375 1589 1891 2272 1375
Size: 214 302 381 3428
Fragments arranged by size:
3428 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2617 2671 4155 4320 50
Size: 2567 54 1484 165 55
Fragments arranged by size:
2567 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1096 1197 2237 1096
Size: 101 1040 3184
Fragments arranged by size:
3184 1040 101
BsrFI r'CCGG_y
Cuts at: 2323 2504 2713 2323
Size: 181 209 3935
Fragments arranged by size:
3935 209 181
BssSI C'ACGA_G
Cuts at: 2596 2954 2596
Size: 358 3967
BstAPI GCAn_nnn'nTGC
Cuts at: 1702 1702
Size: 4325
BstBI TT'CG_AA
Cuts at: 2686 2686
Size: 4325
BstYI r'GATC_y
Cuts at: 1 404 473 542 611 680 749 818 1065
Size: 403 69 69 69 69 69 69 247
Cuts at: 1065 1232 1350 1817 1840 2175 2421 3433 3519
Size: 167 118 467 23 335 246 1012 86
Cuts at: 3519 3531 3877 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 403 346 335 247 246
167 118 86 69 69 69 69 69
69 23 12
BtgI C'CryG_G
Cuts at: 225 225
Size: 4325
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2275 2456 2619 2275
Size: 181 163 3981
Fragments arranged by size:
3981 181 163
BtsI GCAGTG_nn'
Cuts at: 374 443 512 581 650 719 788 857 1568
Size: 69 69 69 69 69 69 69 711
Cuts at: 1568 374
Size: 3131
Fragments arranged by size:
3131 711 69 69 69 69 69 69
69
DraI TTT'AAA
Cuts at: 1324 1324
Size: 4325
DrdI GACnn_nn'nnGTC
Cuts at: 2031 2889 2031
Size: 858 3467
EaeI y'GGCC_r
Cuts at: 222 1910 2084 2475 2502 3847 222
Size: 1688 174 391 27 1345 700
Fragments arranged by size:
1688 1345 700 391 174 27
EagI C'GGCC_G
Cuts at: 1910 1910
Size: 4325
EarI CTCTTCn'nnn_
Cuts at: 2348 2558 2348
Size: 210 4115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2533 2843 2989 4135 2533
Size: 310 146 1146 2723
Fragments arranged by size:
2723 1146 310 146
EcoICRI GAG'CTC
Cuts at: 378 1337 378
Size: 959 3366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 448 517 586 655 724 793 862 1245
Size: 276 69 69 69 69 69 69 383
Cuts at: 1245 1271 2150 2582 3329 3597 172
Size: 26 879 432 747 268 900
Fragments arranged by size:
900 879 747 432 383 276 268 69
69 69 69 69 69 26
EcoNI CCTnn'n_nnAGG
Cuts at: 151 154 1260 151
Size: 3 1106 3216
Fragments arranged by size:
3216 1106 3
EcoO109I rG'GnC_Cy
Cuts at: 294 971 1464 294
Size: 677 493 3155
Fragments arranged by size:
3155 677 493
EcoRI G'AATT_C
Cuts at: 1329 1329
Size: 4325
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2344 2344
Size: 4325
FspI TGC'GCA
Cuts at: 2106 2106
Size: 4325
HaeII r_GCGC'y
Cuts at: 1759 2007 3029 4008 1759
Size: 248 1022 979 2076
Fragments arranged by size:
2076 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2167 2197 2199 2229 2167
Size: 30 2 30 4263
Fragments arranged by size:
4263 30 30 2
HincII GTy'rAC
Cuts at: 1364 1364
Size: 4325
KasI G'GCGC_C
Cuts at: 1755 2003 1755
Size: 248 4077
KpnI G_GTAC'C
Cuts at: 1345 1345
Size: 4325
MluI A'CGCG_T
Cuts at: 3758 3758
Size: 4325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 207 2996 3180 207
Size: 2789 184 1352
Fragments arranged by size:
2789 1352 184
MscI TGG'CCA
Cuts at: 224 2086 224
Size: 1862 2463
MslI CAynn'nnrTG
Cuts at: 1051 2314 2441 1051
Size: 1263 127 2935
Fragments arranged by size:
2935 1263 127
MspA1I CmG'CkG
Cuts at: 141 165 426 840 930 1020 1436 1750 2110
Size: 24 261 414 90 90 416 314 360
Cuts at: 2110 3123 3368 141
Size: 1013 245 1098
Fragments arranged by size:
1098 1013 416 414 360 314 261 245
90 90 24
NaeI GCC'GGC
Cuts at: 2506 2506
Size: 4325
NarI GG'CG_CC
Cuts at: 1756 2004 1756
Size: 248 4077
NcoI C'CATG_G
Cuts at: 225 225
Size: 4325
NgoMIV G'CCGG_C
Cuts at: 2504 2504
Size: 4325
NspI r_CATG'y
Cuts at: 1378 1878 2785 1378
Size: 500 907 2918
Fragments arranged by size:
2918 907 500
PasI CC'CwG_GG
Cuts at: 878 878
Size: 4325
PciI A'CATG_T
Cuts at: 2781 2781
Size: 4325
PflMI CCAn_nnn'nTGG
Cuts at: 1229 1229
Size: 4325
PfoI T'CCnGG_A
Cuts at: 149 149
Size: 4325
PmeI GTTT'AAAC
Cuts at: 1324 1324
Size: 4325
PmlI CAC'GTG
Cuts at: 1085 1085
Size: 4325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3464 3496 3464
Size: 32 4293
PpuMI rG'GwC_Cy
Cuts at: 294 294
Size: 4325
PspOMI G'GGCC_C
Cuts at: 971 971
Size: 4325
PspXI vC'TCGA_Gb
Cuts at: 191 191
Size: 4325
PstI C_TGCA'G
Cuts at: 137 369 401 438 470 507 539 576 645
Size: 232 32 37 32 37 32 37 69
Cuts at: 645 714 783 852 1025 1372 2057 137
Size: 69 69 69 173 347 685 2405
Fragments arranged by size:
2405 685 347 232 173 69 69 69
69 37 37 37 32 32 32
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4325
PvuII CAG'CTG
Cuts at: 141 165 426 840 1020 1750 2110 141
Size: 24 261 414 180 730 360 2356
Fragments arranged by size:
2356 730 414 360 261 180 24
RsrII CG'GwC_CG
Cuts at: 2520 2520
Size: 4325
SacI G_AGCT'C
Cuts at: 380 1339 380
Size: 959 3366
SalI G'TCGA_C
Cuts at: 1362 1362
Size: 4325
SapI GCTCTTCn'nnn_
Cuts at: 2348 2558 2348
Size: 210 4115
SbfI CC_TGCA'GG
Cuts at: 1372 1372
Size: 4325
ScaI AGT'ACT
Cuts at: 3842 3842
Size: 4325
SfcI C'TryA_G
Cuts at: 33 133 365 397 434 466 503 535 572
Size: 100 232 32 37 32 37 32 37
Cuts at: 572 641 710 779 848 1015 1021 1368 2053
Size: 69 69 69 69 167 6 347 685
Cuts at: 2053 3046 3237 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 347 232 191 167 100
69 69 69 69 37 37 37 32
32 32 6
SfoI GGC'GCC
Cuts at: 1757 2005 1757
Size: 248 4077
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4325
SmaI CCC'GGG
Cuts at: 1347 1347
Size: 4325
SmlI C'TyrA_G
Cuts at: 191 1480 2395 2887 3149 191
Size: 1289 915 492 262 1367
Fragments arranged by size:
1367 1289 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4325
SphI G_CATG'C
Cuts at: 1378 1878 1378
Size: 500 3825
StuI AGG'CCT
Cuts at: 173 173
Size: 4325
StyI C'CwwG_G
Cuts at: 119 174 225 290 897 1459 1813 119
Size: 55 51 65 607 562 354 2631
Fragments arranged by size:
2631 607 562 354 65 55 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 361 430 499 568 637 706 775 1083 1908
Size: 69 69 69 69 69 69 308 825
Cuts at: 1908 2041 2500 2709 361
Size: 133 459 209 1977
Fragments arranged by size:
1977 825 459 308 209 133 69 69
69 69 69 69
TatI w'GTAC_w
Cuts at: 3840 3840
Size: 4325
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 847 879 1069 1101 3842 3874 847
Size: 32 190 32 2741 32 1298
Fragments arranged by size:
2741 1298 190 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2122 2122
Size: 4325
XbaI T'CTAG_A
Cuts at: 1356 1356
Size: 4325
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1820 1820
Size: 4325
XhoI C'TCGA_G
Cuts at: 191 191
Size: 4325
XmaI C'CCGG_G
Cuts at: 1345 1345
Size: 4325
XmnI GAAnn'nnTTC
Cuts at: 1277 1277
Size: 4325
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PshAI PsiI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI ZraI