Restriction map for pF1KB6631
 (Linear) (Six-base) MAPSORT of: KIBB6631.seq  Check: 8396  from: 1  to: 1212



KIBB6631.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

AclI AA'CG_TT

Cuts at:      0    1049    1212 
   Size:       1049     163
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340     409     478     547     616     685     754    1212 
   Size:        340      69      69      69      69      69      69     458

  Fragments arranged by size: 

                 458     340      69      69      69      69      69      69
            
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1164    1212 
   Size:       1164      48
 
AlwNI CAG_nnn'CTG

Cuts at:      0      33     240     309     378     447     516     585     654 
   Size:         33     207      69      69      69      69      69      69

Cuts at:    654     723    1186    1212 
   Size:         69     463      26

  Fragments arranged by size: 

                 463     207      69      69      69      69      69      69
                  69      33      26
 
ApaI G_GGCC'C

Cuts at:      0     867    1212 
   Size:        867     345
 
ApoI r'AATT_y

Cuts at:      0    1072    1117    1212 
   Size:       1072      45      95

  Fragments arranged by size: 

                1072      95      45
 
AvaI C'yCGr_G

Cuts at:      0      83    1212 
   Size:         83    1129
 
BanII G_rGCy'C

Cuts at:      0     167     272     867    1043    1212 
   Size:        167     105     595     176     169

  Fragments arranged by size: 

                 595     176     169     167     105
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     911    1067    1212 
   Size:        911     156     145

  Fragments arranged by size: 

                 911     156     145
 
BbvCI CC'TCA_GC

Cuts at:      0     890    1212 
   Size:        890     322
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      82    1212 
   Size:         82    1130
 
BglII A'GATC_T

Cuts at:      0     296     365     434     503     572     641     710     957 
   Size:        296      69      69      69      69      69      69     247

Cuts at:    957    1212 
   Size:        255

  Fragments arranged by size: 

                 296     255     247      69      69      69      69      69
                  69
 
BlpI GC'TnA_GC

Cuts at:      0      99    1212 
   Size:         99    1113
 
Bme1580I G_kGCm'C

Cuts at:      0     867    1212 
   Size:        867     345
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64    1137    1163    1212 
   Size:         64    1073      26      49

  Fragments arranged by size: 

                1073      64      49      26
 
Bpu10I CC'TnA_GC

Cuts at:      0     890    1212 
   Size:        890     322
 
BsaAI yAC'GTr

Cuts at:      0     977    1212 
   Size:        977     235
 
BsaBI GATnn'nnATC

Cuts at:      0     962    1212 
   Size:        962     250
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      61     130     907    1049    1212 
   Size:         61      69     777     142     163

  Fragments arranged by size: 

                 777     163     142      69      61
 
BseYI C'CCAG_C

Cuts at:      0      33     160     190     245     314     728     818     866 
   Size:         33     127      30      55      69     414      90      48

Cuts at:    866    1009    1212 
   Size:        143     203

  Fragments arranged by size: 

                 414     203     143     127      90      69      55      48
                  33      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     272    1212 
   Size:        272     940
 
Bsp1286I G_dGCh'C

Cuts at:      0     167     272     867    1043    1212 
   Size:        167     105     595     176     169

  Fragments arranged by size: 

                 595     176     169     167     105
 
BsrDI GCAATG_nn'

Cuts at:      0     988    1089    1212 
   Size:        988     101     123

  Fragments arranged by size: 

                 988     123     101
 
BstYI r'GATC_y

Cuts at:      0     296     365     434     503     572     641     710     957 
   Size:        296      69      69      69      69      69      69     247

Cuts at:    957    1124    1212 
   Size:        167      88

  Fragments arranged by size: 

                 296     247     167      88      69      69      69      69
                  69      69
 
BtgI C'CryG_G

Cuts at:      0     117    1212 
   Size:        117    1095
 
BtsI GCAGTG_nn'

Cuts at:      0     266     335     404     473     542     611     680     749 
   Size:        266      69      69      69      69      69      69      69

Cuts at:    749    1212 
   Size:        463

  Fragments arranged by size: 

                 463     266      69      69      69      69      69      69
                  69
 
EaeI y'GGCC_r

Cuts at:      0     114    1212 
   Size:        114    1098
 
EcoICRI GAG'CTC

Cuts at:      0     270    1212 
   Size:        270     942
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     340     409     478     547     616     685     754 
   Size:         64     276      69      69      69      69      69      69

Cuts at:    754    1137    1163    1212 
   Size:        383      26      49

  Fragments arranged by size: 

                 383     276      69      69      69      69      69      69
                  64      49      26
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      43      46    1152    1212 
   Size:         43       3    1106      60

  Fragments arranged by size: 

                1106      60      43       3
 
EcoO109I rG'GnC_Cy

Cuts at:      0     186     863    1212 
   Size:        186     677     349

  Fragments arranged by size: 

                 677     349     186
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      99    1212 
   Size:         99    1113
 
MscI TGG'CCA

Cuts at:      0     116    1212 
   Size:        116    1096
 
MslI CAynn'nnrTG

Cuts at:      0     943    1212 
   Size:        943     269
 
MspA1I CmG'CkG

Cuts at:      0      33      57     318     732     822     912    1212 
   Size:         33      24     261     414      90      90     300

  Fragments arranged by size: 

                 414     300     261      90      90      33      24
 
NcoI C'CATG_G

Cuts at:      0     117    1212 
   Size:        117    1095
 
PasI CC'CwG_GG

Cuts at:      0     770    1212 
   Size:        770     442
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1121    1212 
   Size:       1121      91
 
PfoI T'CCnGG_A

Cuts at:      0      41    1212 
   Size:         41    1171
 
PmlI CAC'GTG

Cuts at:      0     977    1212 
   Size:        977     235
 
PpuMI rG'GwC_Cy

Cuts at:      0     186    1212 
   Size:        186    1026
 
PspOMI G'GGCC_C

Cuts at:      0     863    1212 
   Size:        863     349
 
PspXI vC'TCGA_Gb

Cuts at:      0      83    1212 
   Size:         83    1129
 
PstI C_TGCA'G

Cuts at:      0      29     261     293     330     362     399     431     468 
   Size:         29     232      32      37      32      37      32      37

Cuts at:    468     537     606     675     744     917    1212 
   Size:         69      69      69      69     173     295

  Fragments arranged by size: 

                 295     232     173      69      69      69      69      37
                  37      37      32      32      32      29
 
PvuII CAG'CTG

Cuts at:      0      33      57     318     732     912    1212 
   Size:         33      24     261     414     180     300

  Fragments arranged by size: 

                 414     300     261     180      33      24
 
SacI G_AGCT'C

Cuts at:      0     272    1212 
   Size:        272     940
 
SfcI C'TryA_G

Cuts at:      0      25     257     289     326     358     395     427     464 
   Size:         25     232      32      37      32      37      32      37

Cuts at:    464     533     602     671     740     907     913    1212 
   Size:         69      69      69      69     167       6     299

  Fragments arranged by size: 

                 299     232     167      69      69      69      69      37
                  37      37      32      32      32      25       6
 
SmlI C'TyrA_G

Cuts at:      0      83    1212 
   Size:         83    1129
 
StuI AGG'CCT

Cuts at:      0      65    1212 
   Size:         65    1147
 
StyI C'CwwG_G

Cuts at:      0      11      66     117     182     789    1212 
   Size:         11      55      51      65     607     423

  Fragments arranged by size: 

                 607     423      65      55      51      11
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     253     322     391     460     529     598     667     975 
   Size:        253      69      69      69      69      69      69     308

Cuts at:    975    1212 
   Size:        237

  Fragments arranged by size: 

                 308     253     237      69      69      69      69      69
                  69
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     739     771     961     993    1212 
   Size:        739      32     190      32     219

  Fragments arranged by size: 

                 739     219     190      32      32
 
XhoI C'TCGA_G

Cuts at:      0      83    1212 
   Size:         83    1129
 
XmnI GAAnn'nnTTC

Cuts at:      0    1169    1212 
   Size:       1169      43
 

 Enzymes that do cut:

     AclI     AcuI     AlfI    AlwNI     ApaI     ApoI     AvaI    BanII
     BbsI    BbvCI    BciVI    BglII     BlpI Bme1580I     BpmI   Bpu10I
    BsaAI    BsaBI    BseRI    BseYI  BsiHKAI Bsp1286I    BsrDI    BstYI
     BtgI     BtsI     EaeI  EcoICRI  Eco57MI    EcoNI EcoO109I     MmeI
     MscI     MslI   MspA1I     NcoI     PasI    PflMI     PfoI     PmlI
    PpuMI   PspOMI    PspXI     PstI    PvuII     SacI     SfcI     SmlI
     StuI     StyI    TaqII     TstI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BanI     BbeI     BcgI     BclI    BfrBI     BglI
    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaHI    BsaWI
    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI     PmeI
     PpiI    PshAI     PsiI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TatI  Tth111I     XbaI     XcmI     XmaI     ZraI
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