Restriction map for pF1KB6623
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BbvCI       BcgI        BclI        BcnI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspHI       BspLU11I    
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        Hin1I       HpaI        KasI        KpnI        
KspI        MamI        MflI        MluI        MspA1I      
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccII          10  CGCG                 CG^CG
AccIII       1199  TCCGGA               T^CCGGA
AfaI           83  GTAC                 GT^AC
Asp700I       109  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          218  TGGCCA               TGG^CCA
BfaI          691  CTAG                 C^TAG
BglI          220  GCCACCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          778  CTGGAG               CTGGAG(16/14)
BsaWI        1199  TCCGGA               [AT]^CCGG[AT]
BseAI        1199  TCCGGA               T^CCGGA
BseRI        1102  GAGGAG               GAGGAG(10/8)
BspEI        1199  TCCGGA               T^CCGGA
BspMI         602  ACCTGC               ACCTGC(4/8)
BsrDI         513  CATTGC               GCAATG(2/0)
BstUI          10  CGCG                 CG^CG
CfoI          909  GCGC                 GCG^C
DsaI         1016  CCATGG               C^C[GA][CT]GG
EaeI          218  TGGCCA               [CT]^GGCC[GA]
EarI          901  GAAGAG               CTCTTC(1/4)
FauI          156  CCCGC                CCCGC(4/6)
HaeII         908  AGCGCC               [GA]GCGC^[CT]
HapII        1200  CCGG                 C^CGG
HgaI          430  GCGTC                GACGC(5/10)
HhaI          909  GCGC                 GCG^C
HinP1I        909  GCGC                 G^CGC
HincII        367  GTCAAC               GT[CT]^[GA]AC
HindII        367  GTCAAC               GT[CT]^[GA]AC
HindIII       253  AAGCTT               A^AGCTT
HpaII        1200  CCGG                 C^CGG
Ksp632I       901  GAAGAG               CTCTTC(1/4)
MaeI          691  CTAG                 C^TAG
MaeII         164  ACGT                 A^CGT
MfeI          203  CAATTG               C^AATTG
MluNI         218  TGGCCA               TGG^CCA
MroI         1199  TCCGGA               T^CCGGA
MscI          218  TGGCCA               TGG^CCA
MseI          197  TTAA                 T^TAA
MspI         1200  CCGG                 C^CGG
MunI          203  CAATTG               C^AATTG
MvnI           10  CGCG                 CG^CG
NcoI         1016  CCATGG               C^CATGG
NlaIV        1196  GGGTCC               GG[ACGT]^[ACGT]CC
PflMI         210  CCAGTTCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          700  CTGCAG               CTGCA^G
RsaI           83  GTAC                 GT^AC
SfcI          700  CTGCAG               C^T[GA][CT]AG
TfiI          967  GAATC                G^A[AT]TC
Tru9I         197  TTAA                 T^TAA
Tsp45I       1226  GTGAC                ^GT[GC]AC
TspRI         975  CAGTG                CA[GC]TG[ACGT][ACGT]^
Van91I        210  CCAGTTCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          109  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AcsI           75  AAATTT               [GA]^AATT[CT]
              784  AAATTT              
ApoI           75  AAATTT               [GA]^AATT[CT]
              784  AAATTT              
AvaII         180  GGACC                G^G[AT]CC
             1197  GGTCC               
BciVI          49  GGATAC               GTATCC(6/5)
              168  GGATAC              
BsrI          669  ACTGG                ACTGG(1/-1)
              210  CCAGT               
BstXI         329  CCAAGGGGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              518  CCAGACCTCTGG        
Cac8I         426  GCTTGC               GC[ACGT]^[ACGT]GC
              911  GCCTGC              
Cfr13I        180  GGACC                G^G[ACGT]CC
             1197  GGTCC               
DdeI          248  CTAAG                C^T[ACGT]AG
              743  CTGAG               
DpnII         297  GATC                 ^GATC
              385  GATC                
HphI          440  TCACC                GGTGA(8/7)
             1174  TCACC               
MaeIII        929  GTAAC                ^GT[ACGT]AC
             1226  GTGAC               
MboI          297  GATC                 ^GATC
              385  GATC                
MslI           27  CACAGAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1017  CATGGAGATG          
NdeII         297  GATC                 ^GATC
              385  GATC                
Sau3AI        297  GATC                 ^GATC
              385  GATC                
Sau96I        180  GGACC                G^G[ACGT]CC
             1197  GGTCC               
SmlI          892  CTCAAG               C^T[CT][GA]AG
              916  CTCAAG              


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AciI        AluI        BanII       BbsI        BbvI        
BmyI        BpuAI       BsaJI       BsiYI       BslI        
BsmAI       Bsp1286I    BssKI       BstF5I      BstNI       
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HinfI       ItaI        MboII       MnlI        MvaI        
MwoI        NlaIII      PleI        ScrFI       SfaNI       
StyI        TaqI        TseI        Tsp509I     TthHB8I     
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