Restriction map for pF1KB6620
 (Linear) (Six-base) MAPSORT of: KIBB6620.seq  Check: 7662  from: 1  to: 1305



KIBB6620.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

Acc65I G'GTAC_C

Cuts at:      0    1030    1305 
   Size:       1030     275
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     476    1305 
   Size:        476     829
 
AflIII A'CryG_T

Cuts at:      0     244    1305 
   Size:        244    1061
 
AleI CACnn'nnGTG

Cuts at:      0    1253    1305 
   Size:       1253      52
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1271    1305 
   Size:       1271      34
 
AlwNI CAG_nnn'CTG

Cuts at:      0     607     879    1305 
   Size:        607     272     426

  Fragments arranged by size: 

                 607     426     272
 
ApaI G_GGCC'C

Cuts at:      0     408    1279    1305 
   Size:        408     871      26

  Fragments arranged by size: 

                 871     408      26
 
ApoI r'AATT_y

Cuts at:      0     255    1305 
   Size:        255    1050
 
AvaI C'yCGr_G

Cuts at:      0     328     409    1003    1189    1305 
   Size:        328      81     594     186     116

  Fragments arranged by size: 

                 594     328     186     116      81
 
BanI G'GyrC_C

Cuts at:      0      96     193     951    1030    1229    1255    1305 
   Size:         96      97     758      79     199      26      50

  Fragments arranged by size: 

                 758     199      97      96      79      50      26
 
BanII G_rGCy'C

Cuts at:      0      71     158     408     467     923    1196    1279    1305 
   Size:         71      87     250      59     456     273      83      26

  Fragments arranged by size: 

                 456     273     250      87      83      71      59      26
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     367     720    1305 
   Size:        367     353     585

  Fragments arranged by size: 

                 585     367     353
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     319     353    1305 
   Size:        319      34     952

  Fragments arranged by size: 

                 952     319      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     921    1305 
   Size:        921     384
 
BglI GCCn_nnn'nGGC

Cuts at:      0     191     449    1305 
   Size:        191     258     856

  Fragments arranged by size: 

                 856     258     191
 
Bme1580I G_kGCm'C

Cuts at:      0     408     521    1279    1305 
   Size:        408     113     758      26

  Fragments arranged by size: 

                 758     408     113      26
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     310     360     606     781     901     926     928    1114 
   Size:        310      50     246     175     120      25       2     186

Cuts at:   1114    1305 
   Size:        191

  Fragments arranged by size: 

                 310     246     191     186     175     120      50      25
                   2
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     584     914    1145    1305 
   Size:        584     330     231     160

  Fragments arranged by size: 

                 584     330     231     160
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     769    1305 
   Size:        769     536
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1121    1305 
   Size:       1121     184
 
BsaHI Gr'CG_yC

Cuts at:      0     792    1305 
   Size:        792     513
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     979    1009    1305 
   Size:        979      30     296

  Fragments arranged by size: 

                 979     296      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     346     525    1305 
   Size:        346     179     780

  Fragments arranged by size: 

                 780     346     179
 
BseYI C'CCAG_C

Cuts at:      0      54     158     541     830    1134    1305 
   Size:         54     104     383     289     304     171

  Fragments arranged by size: 

                 383     304     289     171     104      54
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     330    1305 
   Size:        330     975
 
BsiHKAI G_wGCw'C

Cuts at:      0     272     467    1250    1305 
   Size:        272     195     783      55

  Fragments arranged by size: 

                 783     272     195      55
 
BsmI GAATG_Cn'

Cuts at:      0    1040    1305 
   Size:       1040     265
 
Bsp1286I G_dGCh'C

Cuts at:      0      71     158     272     408     467     521     923    1196 
   Size:         71      87     114     136      59      54     402     273

Cuts at:   1196    1250    1279    1305 
   Size:         54      29      26

  Fragments arranged by size: 

                 402     273     136     114      87      71      59      54
                  54      29      26
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     133     634    1305 
   Size:        133     501     671

  Fragments arranged by size: 

                 671     501     133
 
BsrFI r'CCGG_y

Cuts at:      0    1051    1305 
   Size:       1051     254
 
BsrGI T'GTAC_A

Cuts at:      0     415    1305 
   Size:        415     890
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     173    1305 
   Size:        173    1132
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1216    1305 
   Size:       1216      89
 
BtgI C'CryG_G

Cuts at:      0    1026    1305 
   Size:       1026     279
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      65    1305 
   Size:         65    1240
 
BtsI GCAGTG_nn'

Cuts at:      0     420     495     605     646     877    1305 
   Size:        420      75     110      41     231     428

  Fragments arranged by size: 

                 428     420     231     110      75      41
 
DraIII CAC_nnn'GTG

Cuts at:      0     569    1305 
   Size:        569     736
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1118    1305 
   Size:       1118     187
 
EaeI y'GGCC_r

Cuts at:      0     683    1305 
   Size:        683     622
 
EarI CTCTTCn'nnn_

Cuts at:      0      67     739    1163    1305 
   Size:         67     672     424     142

  Fragments arranged by size: 

                 672     424     142      67
 
EcoICRI GAG'CTC

Cuts at:      0     465    1305 
   Size:        465     840
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     476     584     914    1145    1305 
   Size:        476     108     330     231     160

  Fragments arranged by size: 

                 476     330     231     160     108
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1269    1305 
   Size:       1269      36
 
EcoO109I rG'GnC_Cy

Cuts at:      0      43     405     808    1120    1176    1275    1305 
   Size:         43     362     403     312      56      99      30

  Fragments arranged by size: 

                 403     362     312      99      56      43      30
 
EcoRI G'AATT_C

Cuts at:      0     255    1305 
   Size:        255    1050
 
HaeII r_GCGC'y

Cuts at:      0     548    1305 
   Size:        548     757
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     767     799    1305 
   Size:        767      32     506

  Fragments arranged by size: 

                 767     506      32
 
KpnI G_GTAC'C

Cuts at:      0    1034    1305 
   Size:       1034     271
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     641     977    1305 
   Size:         92     549     336     328

  Fragments arranged by size: 

                 549     336     328      92
 
MslI CAynn'nnrTG

Cuts at:      0     676    1253    1305 
   Size:        676     577      52

  Fragments arranged by size: 

                 676     577      52
 
MspA1I CmG'CkG

Cuts at:      0     182     297     374     551    1305 
   Size:        182     115      77     177     754

  Fragments arranged by size: 

                 754     182     177     115      77
 
NdeI CA'TA_TG

Cuts at:      0     105    1305 
   Size:        105    1200
 
NspI r_CATG'y

Cuts at:      0     248    1305 
   Size:        248    1057
 
PciI A'CATG_T

Cuts at:      0     244    1305 
   Size:        244    1061
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     585    1305 
   Size:        585     720
 
PfoI T'CCnGG_A

Cuts at:      0     224    1122    1305 
   Size:        224     898     183

  Fragments arranged by size: 

                 898     224     183
 
PpuMI rG'GwC_Cy

Cuts at:      0     808    1120    1176    1305 
   Size:        808     312      56     129

  Fragments arranged by size: 

                 808     312     129      56
 
PspOMI G'GGCC_C

Cuts at:      0     404    1275    1305 
   Size:        404     871      30

  Fragments arranged by size: 

                 871     404      30
 
PspXI vC'TCGA_Gb

Cuts at:      0     328     409    1305 
   Size:        328      81     896

  Fragments arranged by size: 

                 896     328      81
 
PstI C_TGCA'G

Cuts at:      0     130     766    1305 
   Size:        130     636     539

  Fragments arranged by size: 

                 636     539     130
 
PvuII CAG'CTG

Cuts at:      0     297    1305 
   Size:        297    1008
 
SacI G_AGCT'C

Cuts at:      0     467    1305 
   Size:        467     838
 
SanDI GG'GwC_CC

Cuts at:      0     808    1176    1305 
   Size:        808     368     129

  Fragments arranged by size: 

                 808     368     129
 
SapI GCTCTTCn'nnn_

Cuts at:      0     739    1305 
   Size:        739     566
 
SbfI CC_TGCA'GG

Cuts at:      0     130    1305 
   Size:        130    1175
 
ScaI AGT'ACT

Cuts at:      0    1103    1305 
   Size:       1103     202
 
SexAI A'CCwGG_T

Cuts at:      0     565    1305 
   Size:        565     740
 
SfcI C'TryA_G

Cuts at:      0     126     762     818    1305 
   Size:        126     636      56     487

  Fragments arranged by size: 

                 636     487     126      56
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1051    1305 
   Size:       1051     254
 
SmaI CCC'GGG

Cuts at:      0    1005    1191    1305 
   Size:       1005     186     114

  Fragments arranged by size: 

                1005     186     114
 
SmlI C'TyrA_G

Cuts at:      0     328     409     784    1305 
   Size:        328      81     375     521

  Fragments arranged by size: 

                 521     375     328      81
 
StyI C'CwwG_G

Cuts at:      0      10     579    1279    1305 
   Size:         10     569     700      26

  Fragments arranged by size: 

                 700     569      26      10
 
TatI w'GTAC_w

Cuts at:      0     415    1101    1305 
   Size:        415     686     204

  Fragments arranged by size: 

                 686     415     204
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     678    1033    1213    1305 
   Size:        678     355     180      92

  Fragments arranged by size: 

                 678     355     180      92
 
XhoI C'TCGA_G

Cuts at:      0     328     409    1305 
   Size:        328      81     896

  Fragments arranged by size: 

                 896     328      81
 
XmaI C'CCGG_G

Cuts at:      0    1003    1189    1305 
   Size:       1003     186     116

  Fragments arranged by size: 

                1003     186     116
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AleI     AlfI    AlwNI     ApaI     ApoI
     AvaI     BanI    BanII     BbsI     BcgI    BciVI     BglI Bme1580I
     BmrI     BpmI    BpuEI     BsaI    BsaHI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrFI    BsrGI   BstAPI
    BstXI     BtgI    BtgZI     BtsI   DraIII     DrdI     EaeI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII    Hin4I     KpnI
     MmeI     MslI   MspA1I     NdeI     NspI     PciI    PflMI     PfoI
    PpuMI   PspOMI    PspXI     PstI    PvuII     SacI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI    SgrAI     SmaI     SmlI     StyI
     TatI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI    BbvCI     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI
     BplI   Bpu10I    BsaAI    BsaBI    BsaWI    BsiEI    BsiWI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI   BssHII    BssSI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I     ClaI    CspCI     DraI     EagI     EciI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     MfeI     MluI     MscI     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PmeI     PmlI
     PpiI    PshAI     PsiI     PsrI     PvuI    RsrII    SacII     SalI
     SfiI     SfoI     SgfI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XmnI     ZraI
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