Restriction map for pF1KB6612
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BbvCI       BcgI        BclI        BcnI        BfrI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaHI       
BsiEI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       HgaI        Hin1I       
HincII      HindII      HpaI        KasI        KpnI        
MfeI        MluI        MslI        MunI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SalI        SapI        SbfI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccIII       1008  TCCGGA               T^CCGGA
AcsI          741  AAATTC               [GA]^AATT[CT]
ApoI          741  AAATTC               [GA]^AATT[CT]
Asp700I       805  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI         1215  TGGCCA               TGG^CCA
BbsI          910  GAAGAC               GAAGAC(2/6)
BglII         935  AGATCT               A^GATCT
BpmI          586  CTGGAG               CTGGAG(16/14)
BpuAI         910  GAAGAC               GAAGAC(2/6)
BsaBI          36  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1225  GGTCTC               GGTCTC(1/5)
BsaWI        1008  TCCGGA               [AT]^CCGG[AT]
BseAI        1008  TCCGGA               T^CCGGA
BsgI          578  CTGCAC               GTGCAG(16/14)
BspEI        1008  TCCGGA               T^CCGGA
BspMI         298  ACCTGC               ACCTGC(4/8)
BsrI          860  CCAGT                ACTGG(1/-1)
BstAPI        220  GCAACAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1093  TTCGAA               TT^CGAA
BstEII       1049  GGTCACC              G^GT[ACGT]ACC
BstF5I        473  GGATG                GGATG(2/0)
BstPI        1049  GGTCACC              G^GT[ACGT]ACC
CfoI          419  GCGC                 GCG^C
DraIII         45  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         1215  TGGCCA               [CT]^GGCC[GA]
EcoO65I      1049  GGTCACC              G^GT[ACGT]ACC
EcoT14I       735  CCTTGG               C^C[AT][AT]GG
FokI          473  GGATG                GGATG(9/13)
HapII        1009  CCGG                 C^CGG
HhaI          419  GCGC                 GCG^C
HinP1I        419  GCGC                 G^CGC
HindIII       787  AAGCTT               A^AGCTT
HpaII        1009  CCGG                 C^CGG
KspI            8  CCGCGG               CCGC^GG
MaeII         173  ACGT                 A^CGT
MamI           36  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1215  TGGCCA               TGG^CCA
MroI         1008  TCCGGA               T^CCGGA
MscI         1215  TGGCCA               TGG^CCA
MspI         1009  CCGG                 C^CGG
NspI          302  GCATGC               [GA]CATG^[CT]
NspV         1093  TTCGAA               TT^CGAA
PflMI         462  CCAGGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1037  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          572  CTGCAG               CTGCA^G
PvuII         575  CAGCTG               CAG^CTG
SacI          634  GAGCTC               GAGCT^C
SacII           8  CCGCGG               CCGC^GG
ScaI          289  AGTACT               AGT^ACT
SfcI          572  CTGCAG               C^T[GA][CT]AG
SfuI         1093  TTCGAA               TT^CGAA
SmlI          496  CTCAAG               C^T[CT][GA]AG
SphI          302  GCATGC               GCATG^C
StyI          735  CCTTGG               C^C[AT][AT]GG
Tsp509I       742  AATT                 ^AATT
Van91I        462  CCAGGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          805  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII           9  CGCG                 CG^CG
              137  CGCG                
AfaI          290  GTAC                 GT^AC
              948  GTAC                
AlwI          751  GATCC                GGATC(4/5)
              339  GGATC               
AlwNI         463  CAGGGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1148  CAGAGCCTG           
AvaI          412  CCCGAG               C^[CT]CG[GA]G
              902  CTCGGG              
BciVI         426  GGATAC               GTATCC(6/5)
              731  GTATCC              
BfaI          113  CTAG                 C^TAG
              212  CTAG                
BglI           17  GCCTGCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              596  GCCACAGTGGC         
BsmAI         900  GTCTC                GTCTC(1/5)
             1226  GTCTC               
BsoBI         412  CCCGAG               C^[CT]CG[GA]G
              902  CTCGGG              
BstUI           9  CGCG                 CG^CG
              137  CGCG                
BstYI         339  GGATCT               [GA]^GATC[CT]
              935  AGATCT              
DdeI          665  CTGAG                C^T[ACGT]AG
              939  CTCAG               
DsaI            8  CCGCGG               C^C[GA][CT]GG
             1221  CCACGG              
FauI            7  CCCGC                CCCGC(4/6)
               10  GCGGG               
MaeI          113  CTAG                 C^TAG
              212  CTAG                
MflI          339  GGATCT               [GA]^GATC[CT]
              935  AGATCT              
MvnI            9  CGCG                 CG^CG
              137  CGCG                
RsaI          290  GTAC                 GT^AC
              948  GTAC                
SfaNI          36  GATGC                GCATC(5/9)
              226  GATGC               
TaqI          628  TCGA                 T^CGA
             1094  TCGA                
TfiI          349  GATTC                G^A[AT]TC
              404  GAATC               
TthHB8I       628  TCGA                 T^CGA
             1094  TCGA                
XhoII         339  GGATCT               [GA]^GATC[CT]
              935  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AciI        AluI        ApaI        AspHI       AvaII       
BanII       BbvI        BmyI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmFI       Bsp1286I    
BssKI       BstNI       Cac8I       Cfr13I      DpnII       
DraII       EarI        EcoO109I    EcoRII      Fnu4HI      
HaeIII      HinfI       HphI        ItaI        Ksp632I     
MaeIII      MboI        MboII       MnlI        MseI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        Sau3AI      Sau96I      ScrFI       
Tru9I       TseI        Tsp45I      TspRI       
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