Restriction map for pF1KB6607
 (Linear) (Six-base) MAPSORT of: KIBB6607.seq  Check: 224  from: 1  to: 1227



KIBB6607.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     375     423    1227 
   Size:        375      48     804

  Fragments arranged by size: 

                 804     375      48
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     740     925    1157    1227 
   Size:         34     706     185     232      70

  Fragments arranged by size: 

                 706     232     185      70      34
 
AleI CACnn'nnGTG

Cuts at:      0     316    1227 
   Size:        316     911
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     638    1227 
   Size:        638     589
 
AlwNI CAG_nnn'CTG

Cuts at:      0     277     490     766    1054    1227 
   Size:        277     213     276     288     173

  Fragments arranged by size: 

                 288     277     276     213     173
 
ApaLI G'TGCA_C

Cuts at:      0     545    1227 
   Size:        545     682
 
ApoI r'AATT_y

Cuts at:      0     829    1182    1227 
   Size:        829     353      45

  Fragments arranged by size: 

                 829     353      45
 
AseI AT'TA_AT

Cuts at:      0     136    1227 
   Size:        136    1091
 
AvaI C'yCGr_G

Cuts at:      0      55    1227 
   Size:         55    1172
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1141    1174    1227 
   Size:       1141      33      53

  Fragments arranged by size: 

                1141      53      33
 
BanII G_rGCy'C

Cuts at:      0     747    1227 
   Size:        747     480
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      22     746    1227 
   Size:         22     724     481

  Fragments arranged by size: 

                 724     481      22
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     901     935    1227 
   Size:        901      34     292

  Fragments arranged by size: 

                 901     292      34
 
Bme1580I G_kGCm'C

Cuts at:      0     549    1227 
   Size:        549     678
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     749    1227 
   Size:        749     478
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       7     365     710     833    1227 
   Size:          7     358     345     123     394

  Fragments arranged by size: 

                 394     358     345     123       7
 
Bpu10I CC'TnA_GC

Cuts at:      0     323    1227 
   Size:        323     904
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     710    1227 
   Size:        710     517
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     729     759    1227 
   Size:        729      30     468

  Fragments arranged by size: 

                 729     468      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      73     737    1031    1034    1227 
   Size:         73     664     294       3     193

  Fragments arranged by size: 

                 664     294     193      73       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     395     443    1227 
   Size:        395      48     784

  Fragments arranged by size: 

                 784     395      48
 
BsiHKAI G_wGCw'C

Cuts at:      0     549    1227 
   Size:        549     678
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      55    1227 
   Size:         55    1172
 
Bsp1286I G_dGCh'C

Cuts at:      0     549     747    1227 
   Size:        549     198     480

  Fragments arranged by size: 

                 549     480     198
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     115     375     417     423    1227 
   Size:        115     260      42       6     804

  Fragments arranged by size: 

                 804     260     115      42       6
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     544     766    1227 
   Size:        544     222     461

  Fragments arranged by size: 

                 544     461     222
 
BstEII G'GTnAC_C

Cuts at:      0       6     219    1227 
   Size:          6     213    1008

  Fragments arranged by size: 

                1008     213       6
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     318    1227 
   Size:        318     909
 
Bsu36I CC'TnA_GG

Cuts at:      0    1091    1227 
   Size:       1091     136
 
BtsI GCAGTG_nn'

Cuts at:      0     242     668     767    1041    1227 
   Size:        242     426      99     274     186

  Fragments arranged by size: 

                 426     274     242     186      99
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     561    1227 
   Size:        561     666
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       7      34     365     710     740     833     925    1157 
   Size:          7      27     331     345      30      93      92     232

Cuts at:   1157    1227 
   Size:         70

  Fragments arranged by size: 

                 345     331     232      93      92      70      30      27
                   7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     484     516     577     609    1227 
   Size:        484      32      61      32     618

  Fragments arranged by size: 

                 618     484      61      32      32
 
HincII GTy'rAC

Cuts at:      0     717    1227 
   Size:        717     510
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     355    1227 
   Size:        355     872
 
MslI CAynn'nnrTG

Cuts at:      0     316     595    1227 
   Size:        316     279     632

  Fragments arranged by size: 

                 632     316     279
 
MspA1I CmG'CkG

Cuts at:      0     772    1227 
   Size:        772     455
 
NdeI CA'TA_TG

Cuts at:      0    1067    1227 
   Size:       1067     160
 
PstI C_TGCA'G

Cuts at:      0     447     771     777    1227 
   Size:        447     324       6     450

  Fragments arranged by size: 

                 450     447     324       6
 
PvuII CAG'CTG

Cuts at:      0     772    1227 
   Size:        772     455
 
ScaI AGT'ACT

Cuts at:      0     738    1227 
   Size:        738     489
 
SfcI C'TryA_G

Cuts at:      0     287     443     767     773     907    1106    1204    1227 
   Size:        287     156     324       6     134     199      98      23

  Fragments arranged by size: 

                 324     287     199     156     134      98      23       6
            
 
SmlI C'TyrA_G

Cuts at:      0     725    1227 
   Size:        725     502
 
StyI C'CwwG_G

Cuts at:      0     347    1227 
   Size:        347     880
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     506    1227 
   Size:        506     721
 
TatI w'GTAC_w

Cuts at:      0     496     736    1227 
   Size:        496     240     491

  Fragments arranged by size: 

                 496     491     240
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     315    1227 
   Size:        315     912
 
XmnI GAAnn'nnTTC

Cuts at:      0     992    1227 
   Size:        992     235
 

 Enzymes that do cut:

     AarI     AcuI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
     AvaI     BaeI    BanII     BbsI     BcgI Bme1580I     BmrI     BpmI
   Bpu10I    BpuEI    BsaXI    BseRI     BsgI  BsiHKAI    BsmBI Bsp1286I
    BspMI   BstAPI   BstEII    BstXI   Bsu36I     BtsI     DrdI  Eco57MI
    Hin4I   HincII     MmeI     MslI   MspA1I     NdeI     PstI    PvuII
     ScaI     SfcI     SmlI     StyI    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI     ApaI     AscI    AsiSI    AvrII    BamHI     BanI
     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI    BstYI  BstZ17I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XbaI     XhoI     XmaI     ZraI
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