Restriction map for pF1KB6593
 (Linear) (Six-base) MAPSORT of: KIBB6593.seq  Check: 1131  from: 1  to: 1131



KIBB6593.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     779     802     988    1131 
   Size:        310     469      23     186     143

  Fragments arranged by size: 

                 469     310     186     143      23
 
AflIII A'CryG_T

Cuts at:      0     473    1131 
   Size:        473     658
 
AgeI A'CCGG_T

Cuts at:      0     496    1131 
   Size:        496     635
 
AleI CACnn'nnGTG

Cuts at:      0      19    1131 
   Size:         19    1112
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     982    1014    1131 
   Size:        982      32     117

  Fragments arranged by size: 

                 982     117      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     202     724    1131 
   Size:        202     522     407

  Fragments arranged by size: 

                 522     407     202
 
ApaI G_GGCC'C

Cuts at:      0     530    1131 
   Size:        530     601
 
ApoI r'AATT_y

Cuts at:      0     613    1131 
   Size:        613     518
 
AvaI C'yCGr_G

Cuts at:      0     994    1131 
   Size:        994     137
 
BamHI G'GATC_C

Cuts at:      0    1035    1131 
   Size:       1035      96
 
BanI G'GyrC_C

Cuts at:      0     580     598    1131 
   Size:        580      18     533

  Fragments arranged by size: 

                 580     533      18
 
BanII G_rGCy'C

Cuts at:      0     530    1072    1131 
   Size:        530     542      59

  Fragments arranged by size: 

                 542     530      59
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     767    1127    1131 
   Size:        767     360       4

  Fragments arranged by size: 

                 767     360       4
 
BbvCI CC'TCA_GC

Cuts at:      0     731    1131 
   Size:        731     400
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     220    1131 
   Size:        220     911
 
BglI GCCn_nnn'nGGC

Cuts at:      0     283     525     709    1084    1131 
   Size:        283     242     184     375      47

  Fragments arranged by size: 

                 375     283     242     184      47
 
BlpI GC'TnA_GC

Cuts at:      0     933    1131 
   Size:        933     198
 
Bme1580I G_kGCm'C

Cuts at:      0     530    1131 
   Size:        530     601
 
BmgBI CAC'GTC

Cuts at:      0     981    1131 
   Size:        981     150
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     340     524     623     761     827    1131 
   Size:        340     184      99     138      66     304

  Fragments arranged by size: 

                 340     304     184     138      99      66
 
Bpu10I CC'TnA_GC

Cuts at:      0     641     731     832    1131 
   Size:        641      90     101     299

  Fragments arranged by size: 

                 641     299     101      90
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     376    1131 
   Size:        376     755
 
BsaBI GATnn'nnATC

Cuts at:      0     322     790    1131 
   Size:        322     468     341

  Fragments arranged by size: 

                 468     341     322
 
BsaWI w'CCGG_w

Cuts at:      0     496    1131 
   Size:        496     635
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     504     534    1131 
   Size:        504      30     597

  Fragments arranged by size: 

                 597     504      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     451     709     712     979    1131 
   Size:        451     258       3     267     152

  Fragments arranged by size: 

                 451     267     258     152       3
 
BseYI C'CCAG_C

Cuts at:      0      83     588    1131 
   Size:         83     505     543

  Fragments arranged by size: 

                 543     505      83
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     243    1131 
   Size:        243     888
 
Bsp1286I G_dGCh'C

Cuts at:      0     530    1072    1131 
   Size:        530     542      59

  Fragments arranged by size: 

                 542     530      59
 
BsrDI GCAATG_nn'

Cuts at:      0     326    1131 
   Size:        326     805
 
BsrFI r'CCGG_y

Cuts at:      0     496    1131 
   Size:        496     635
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     724    1131 
   Size:        724     407
 
BstBI TT'CG_AA

Cuts at:      0    1056    1131 
   Size:       1056      75
 
BstYI r'GATC_y

Cuts at:      0     366     624    1035    1131 
   Size:        366     258     411      96

  Fragments arranged by size: 

                 411     366     258      96
 
EaeI y'GGCC_r

Cuts at:      0     204     810     900    1131 
   Size:        204     606      90     231

  Fragments arranged by size: 

                 606     231     204      90
 
EarI CTCTTCn'nnn_

Cuts at:      0     428     668     845    1131 
   Size:        428     240     177     286

  Fragments arranged by size: 

                 428     286     240     177
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     922    1131 
   Size:        922     209
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     779     802     988    1131 
   Size:        310     469      23     186     143

  Fragments arranged by size: 

                 469     310     186     143      23
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     394    1131 
   Size:        394     737
 
EcoO109I rG'GnC_Cy

Cuts at:      0     990    1131 
   Size:        990     141
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     270    1131 
   Size:        270     861
 
FspI TGC'GCA

Cuts at:      0     322    1131 
   Size:        322     809
 
FspAI rTGC'GCAy

Cuts at:      0     322    1131 
   Size:        322     809
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     504     536     916     948    1131 
   Size:        504      32     380      32     183

  Fragments arranged by size: 

                 504     380     183      32      32
 
HincII GTy'rAC

Cuts at:      0      99     414    1131 
   Size:         99     315     717

  Fragments arranged by size: 

                 717     315      99
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     258    1131 
   Size:        258     873
 
MscI TGG'CCA

Cuts at:      0     902    1131 
   Size:        902     229
 
MslI CAynn'nnrTG

Cuts at:      0      19     271     874    1131 
   Size:         19     252     603     257

  Fragments arranged by size: 

                 603     257     252      19
 
MspA1I CmG'CkG

Cuts at:      0     681     687     715    1131 
   Size:        681       6      28     416

  Fragments arranged by size: 

                 681     416      28       6
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     202    1131 
   Size:        202     929
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     486     518    1131 
   Size:        486      32     613

  Fragments arranged by size: 

                 613     486      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     990    1131 
   Size:        990     141
 
PspOMI G'GGCC_C

Cuts at:      0     526    1131 
   Size:        526     605
 
PstI C_TGCA'G

Cuts at:      0      80     720     887    1131 
   Size:         80     640     167     244

  Fragments arranged by size: 

                 640     244     167      80
 
PvuII CAG'CTG

Cuts at:      0     715    1131 
   Size:        715     416
 
SapI GCTCTTCn'nnn_

Cuts at:      0     668     845    1131 
   Size:        668     177     286

  Fragments arranged by size: 

                 668     286     177
 
SbfI CC_TGCA'GG

Cuts at:      0     887    1131 
   Size:        887     244
 
SfcI C'TryA_G

Cuts at:      0      76     716     883    1131 
   Size:         76     640     167     248

  Fragments arranged by size: 

                 640     248     167      76
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1084    1131 
   Size:       1084      47
 
SmlI C'TyrA_G

Cuts at:      0     391    1131 
   Size:        391     740
 
StyI C'CwwG_G

Cuts at:      0     239     650     959    1091    1131 
   Size:        239     411     309     132      40

  Fragments arranged by size: 

                 411     309     239     132      40
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     332     364     807     839    1131 
   Size:        332      32     443      32     292

  Fragments arranged by size: 

                 443     332     292      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     805     936    1131 
   Size:        805     131     195

  Fragments arranged by size: 

                 805     195     131
 
XmnI GAAnn'nnTTC

Cuts at:      0     498    1052    1131 
   Size:        498     554      79

  Fragments arranged by size: 

                 554     498      79
 

 Enzymes that do cut:

     AcuI   AflIII     AgeI     AleI     AloI    AlwNI     ApaI     ApoI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI    BciVI     BglI
     BlpI Bme1580I    BmgBI     BmrI   Bpu10I    BpuEI    BsaBI    BsaWI
    BsaXI    BseRI    BseYI     BsgI Bsp1286I    BsrDI    BsrFI   BstAPI
    BstBI    BstYI     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I
     FalI     FspI    FspAI    Hin4I   HincII     MmeI     MscI     MslI
   MspA1I    PflMI     PpiI    PpuMI   PspOMI     PstI    PvuII     SapI
     SbfI     SfcI     SfiI     SmlI     StyI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AhdI
     AlfI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
     BcgI     BclI    BfrBI    BglII     BmtI     BplI     BpmI     BsaI
    BsaAI    BsaHI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrGI   BssHII    BssSI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI  EcoICRI    EcoRI    EcoRV     FseI    HaeII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PmlI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     ScaI    SexAI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI  Tth111I
     XbaI     XhoI     XmaI     ZraI
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