Restriction map for pF1KB6302
##################################
# NO CUT ENZYMES (  175 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HhaI        Hin1I       HinP1I      
HincII      HindII      HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MunI        MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StyI        SwaI        XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AciI          451  GCGG                 CCGC(-3/-1)
AspHI         183  GAGCAC               G[AT]GC[AT]^C
AspI          721  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BcnI          234  CCGGG                CC^[GC]GG
BglII         545  AGATCT               A^GATCT
BmyI          183  GAGCAC               G[AGT]GC[ACT]^C
BpmI           21  CTGGAG               CTGGAG(16/14)
BsaJI         195  CCAGGG               C^C[ACGT][ACGT]GG
BsgI          413  GTGCAG               GTGCAG(16/14)
BsiHKAI       183  GAGCAC               G[AT]GC[AT]^C
Bsp1286I      183  GAGCAC               G[AGT]GC[ACT]^C
Cfr13I        192  GGGCC                G^G[ACGT]CC
EarI          147  GAAGAG               CTCTTC(1/4)
HapII         234  CCGG                 C^CGG
HgaI          408  GCGTC                GACGC(5/10)
HindIII       174  AAGCTT               A^AGCTT
HpaII         234  CCGG                 C^CGG
Ksp632I       147  GAAGAG               CTCTTC(1/4)
MaeII         583  ACGT                 A^CGT
MslI          553  CACCTCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          234  CCGG                 C^CGG
NciI          234  CCGGG                CC^[GC]GG
NlaIII        354  CATG                 CATG^
PflFI         721  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         630  CCAGCGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          755  CTGCAG               CTGCA^G
Sau96I        192  GGGCC                G^G[ACGT]CC
SexAI         701  ACCTGGT              A^CC[AT]GGT
SfaNI         589  GCATC                GCATC(5/9)
StuI          264  AGGCCT               AGG^CCT
TfiI           67  GAATC                G^A[AT]TC
Tth111I       721  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        630  CCAGCGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwI          350  GGATC                GGATC(4/5)
              690  GGATC               
AlwNI          15  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              631  CAGCGACTG           
BfaI          142  CTAG                 C^TAG
              448  CTAG                
BseRI         475  GAGGAG               GAGGAG(10/8)
              793  GAGGAG              
BstF5I        777  GGATG                GGATG(2/0)
              810  GGATG               
BstXI         249  CCACACTTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              650  CCAAAGGAATGG        
BstYI         545  AGATCT               [GA]^GATC[CT]
              690  GGATCT              
FokI          777  GGATG                GGATG(9/13)
              810  GGATG               
MaeI          142  CTAG                 C^TAG
              448  CTAG                
MaeIII        580  GTCAC                ^GT[ACGT]AC
              719  GTGAC               
MflI          545  AGATCT               [GA]^GATC[CT]
              690  GGATCT              
MseI          167  TTAA                 T^TAA
              461  TTAA                
MspA1I         15  CAGCTG               C[AC]G^C[GT]G
              784  CAGCTG              
MwoI          176  GCTTTGAGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              185  GCACTGTGGGC         
PvuII          15  CAGCTG               CAG^CTG
              784  CAGCTG              
SfcI           12  CTACAG               C^T[GA][CT]AG
              755  CTGCAG              
TaqI          438  TCGA                 T^CGA
              744  TCGA                
Tru9I         167  TTAA                 T^TAA
              461  TTAA                
Tsp45I        580  GTCAC                ^GT[GC]AC
              719  GTGAC               
Tsp509I       346  AATT                 ^AATT
              457  AATT                
TthHB8I       438  TCGA                 T^CGA
              744  TCGA                
XhoII         545  AGATCT               [GA]^GATC[CT]
              690  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AluI        BbvI        BsiYI       BslI        BsmAI       
BsrI        BssKI       BstNI       Cac8I       DdeI        
DpnII       EcoRII      Fnu4HI      HaeIII      HinfI       
HphI        ItaI        MboI        MboII       MnlI        
MvaI        NdeII       NlaIV       PleI        Sau3AI      
ScrFI       TseI        TspRI       
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