Restriction map for pF1KB6136
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AflII       
AflIII      AgeI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp700I     AspI        
AviII       AvrII       BbrPI       BbsI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaI        BseAI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      EagI        
EclXI       Eco47III    Eco52I      EcoO65I     EcoRV       
EcoT14I     EcoT22I     FseI        FspI        HpaI        
KspI        MfeI        MluI        MroI        MseI        
MslI        MunI        NaeI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    RsrII       SacII       SalI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SnaBI       
SpeI        SphI        SspBI       StyI        SwaI        
TfiI        Tru9I       Tth111I     Van91I      XbaI        
XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

BamHI        1092  GGATCC               G^GATCC
BcgI         1697  GCATCAACCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1370  GTATCC               GTATCC(6/5)
BclI          999  TGATCA               T^GATCA
BfaI         1582  CTAG                 C^TAG
BsaBI         556  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI         543  CGATCG               CG[GA][CT]^CG
BspHI          50  TCATGA               T^CATGA
BsrBI          46  CCGCTC               CCGCTC(-3/-3)
BstXI         152  CCAAGCCTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI          411  GACTGACTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1520  CCGTGG               C^C[GA][CT]GG
EarI         1420  CTCTTC               CTCTTC(1/4)
FbaI          999  TGATCA               T^GATCA
HgaI         1870  GACGC                GACGC(5/10)
HincII        772  GTTGAC               GT[CT]^[GA]AC
HindII        772  GTTGAC               GT[CT]^[GA]AC
HindIII      1109  AAGCTT               A^AGCTT
Ksp632I      1420  CTCTTC               CTCTTC(1/4)
MaeI         1582  CTAG                 C^TAG
MaeII         946  ACGT                 A^CGT
MamI          556  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspI         1751  ACATGC               [GA]CATG^[CT]
PaeR7I       1645  CTCGAG               C^TCGAG
PpuMI        1046  GGGACCT              [GA]G^G[AT]CC[CT]
PvuI          543  CGATCG               CGAT^CG
RcaI           50  TCATGA               T^CATGA
SapI         1419  GCTCTTC              GCTCTTC(1/4)
SbfI          198  CCTGCAGG             CCTGCA^GG
SmaI         1775  CCCGGG               CCC^GGG
SmlI         1645  CTCGAG               C^T[CT][GA]AG
Sse8387I      198  CCTGCAGG             CCTGCA^GG
SspI          485  AATATT               AAT^ATT
XhoI         1645  CTCGAG               C^TCGAG
XmaCI        1775  CCCGGG               C^CCGGG
XmaI         1775  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

Acc65I        968  GGTACC               G^GTACC
             1126  GGTACC              
AccII        1872  CGCG                 CG^CG
             1874  CGCG                
AcyI         1031  GGCGCC               G[GA]^CG[CT]C
             1585  GGCGCC              
AhdI          448  GACCCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              940  GACCTTACGTC         
AlwNI        1117  CAGTATCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1622  CAGTAGCTG           
AocI          526  CCTGAGG              CC^T[ACGT]AGG
              568  CCTGAGG             
Asp718I       968  GGTACC               G^GTACC
             1126  GGTACC              
AspEI         448  GACCCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              940  GACCTTACGTC         
BalI          605  TGGCCA               TGG^CCA
             1595  TGGCCA              
BbeI         1031  GGCGCC               GGCGC^C
             1585  GGCGCC              
BsaHI        1031  GGCGCC               G[GA]^CG[CT]C
             1585  GGCGCC              
BsaWI         691  TCCGGT               [AT]^CCGG[AT]
             1667  TCCGGT              
BsmAI         409  GAGAC                GTCTC(1/5)
             1280  GAGAC               
BsmI           28  GAATGC               GAATGC(1/-1)
              210  GAATGC              
BsrDI        1172  CATTGC               GCAATG(2/0)
                4  GCAATG              
BstAPI       1621  GCAGTAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1688  GCATCAGCTGC         
BstUI        1872  CGCG                 CG^CG
             1874  CGCG                
Bsu36I        526  CCTGAGG              CC^T[ACGT]AGG
              568  CCTGAGG             
Eam1105I      448  GACCCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              940  GACCTTACGTC         
Eco81I        526  CCTGAGG              CC^T[ACGT]AGG
              568  CCTGAGG             
EcoNI          87  CCTTCGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1035  CCTTCAGGAGG         
EcoRI         193  GAATTC               G^AATTC
              427  GAATTC              
Hin1I        1031  GGCGCC               G[GA]^CG[CT]C
             1585  GGCGCC              
KasI         1031  GGCGCC               G^GCGCC
             1585  GGCGCC              
KpnI          968  GGTACC               GGTAC^C
             1126  GGTACC              
MluNI         605  TGGCCA               TGG^CCA
             1595  TGGCCA              
MscI          605  TGGCCA               TGG^CCA
             1595  TGGCCA              
MvnI         1872  CGCG                 CG^CG
             1874  CGCG                
NarI         1031  GGCGCC               GG^CGCC
             1585  GGCGCC              
SfoI         1031  GGCGCC               GGC^GCC
             1585  GGCGCC              
StuI          106  AGGCCT               AGG^CCT
             1262  AGGCCT              
TaqI          854  TCGA                 T^CGA
             1646  TCGA                
Tsp45I        596  GTGAC                ^GT[GC]AC
             1318  GTGAC               
TthHB8I       854  TCGA                 T^CGA
             1646  TCGA                
XcmI          309  CCAGAGAGCCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              593  CCAGTGACTTTGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   81 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApaI        ApoI        AspHI       AvaI        AvaII       
BanI        BanII       BbvCI       BbvI        BcnI        
BglI        BmyI        BpmI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmFI       
BsoBI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EaeI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MflI        MnlI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PstI        PvuII       
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TseI        Tsp509I     TspRI       
XhoII       
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