Restriction map for pF1KB6117
 (Linear) (Six-base) MAPSORT of: KIBB6117.seq  Check: 7635  from: 1  to: 1863



KIBB6117.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     305     794    1863 
   Size:        305     489    1069

  Fragments arranged by size: 

                1069     489     305
 
AlwNI CAG_nnn'CTG

Cuts at:      0     991    1369    1863 
   Size:        991     378     494

  Fragments arranged by size: 

                 991     494     378
 
ApoI r'AATT_y

Cuts at:      0     827    1214    1249    1863 
   Size:        827     387      35     614

  Fragments arranged by size: 

                 827     614     387      35
 
BanI G'GyrC_C

Cuts at:      0    1539    1863 
   Size:       1539     324
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1451    1863 
   Size:       1451     412
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1035    1317    1863 
   Size:       1035     282     546

  Fragments arranged by size: 

                1035     546     282
 
BclI T'GATC_A

Cuts at:      0     948    1863 
   Size:        948     915
 
BfrBI ATG'CAT

Cuts at:      0     575    1469    1863 
   Size:        575     894     394

  Fragments arranged by size: 

                 894     575     394
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1381    1863 
   Size:       1381     482
 
Bme1580I G_kGCm'C

Cuts at:      0    1039    1544    1863 
   Size:       1039     505     319

  Fragments arranged by size: 

                1039     505     319
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1538    1677    1863 
   Size:       1538     139     186

  Fragments arranged by size: 

                1538     186     139
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     647    1863 
   Size:        647    1216
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1275    1863 
   Size:       1275     588
 
BsaBI GATnn'nnATC

Cuts at:      0     166     674    1863 
   Size:        166     508    1189

  Fragments arranged by size: 

                1189     508     166
 
BsaHI Gr'CG_yC

Cuts at:      0     186     774    1863 
   Size:        186     588    1089

  Fragments arranged by size: 

                1089     588     186
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     457    1094    1863 
   Size:        457     637     769

  Fragments arranged by size: 

                 769     637     457
 
BsiHKAI G_wGCw'C

Cuts at:      0     963    1863 
   Size:        963     900
 
Bsp1286I G_dGCh'C

Cuts at:      0     963    1039    1544    1863 
   Size:        963      76     505     319

  Fragments arranged by size: 

                 963     505     319      76
 
BsrBI CCG'CTC

Cuts at:      0     140    1863 
   Size:        140    1723
 
BsrGI T'GTAC_A

Cuts at:      0    1084    1321    1863 
   Size:       1084     237     542

  Fragments arranged by size: 

                1084     542     237
 
BssSI C'ACGA_G

Cuts at:      0     489    1863 
   Size:        489    1374
 
BstBI TT'CG_AA

Cuts at:      0      11    1863 
   Size:         11    1852
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1261    1863 
   Size:       1261     602
 
BstYI r'GATC_y

Cuts at:      0     987    1863 
   Size:        987     876
 
BtgI C'CryG_G

Cuts at:      0     290    1863 
   Size:        290    1573
 
BtsI GCAGTG_nn'

Cuts at:      0    1082    1102    1833    1863 
   Size:       1082      20     731      30

  Fragments arranged by size: 

                1082     731      30      20
 
EaeI y'GGCC_r

Cuts at:      0     287    1863 
   Size:        287    1576
 
EarI CTCTTCn'nnn_

Cuts at:      0       8     130    1433    1434    1863 
   Size:          8     122    1303       1     429

  Fragments arranged by size: 

                1303     429     122       8       1
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19    1565    1863 
   Size:         19    1546     298

  Fragments arranged by size: 

                1546     298      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     305     794    1863 
   Size:        305     489    1069

  Fragments arranged by size: 

                1069     489     305
 
EcoRV GAT'ATC

Cuts at:      0     193    1863 
   Size:        193    1670
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     994    1863 
   Size:        994     869
 
HaeII r_GCGC'y

Cuts at:      0     124     768    1863 
   Size:        124     644    1095

  Fragments arranged by size: 

                1095     644     124
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     247     279    1837    1863 
   Size:        247      32    1558      26

  Fragments arranged by size: 

                1558     247      32      26
 
MslI CAynn'nnrTG

Cuts at:      0     431    1474    1863 
   Size:        431    1043     389

  Fragments arranged by size: 

                1043     431     389
 
MspA1I CmG'CkG

Cuts at:      0     814    1863 
   Size:        814    1049
 
NsiI A_TGCA'T

Cuts at:      0     577    1471    1863 
   Size:        577     894     392

  Fragments arranged by size: 

                 894     577     392
 
NspI r_CATG'y

Cuts at:      0    1632    1863 
   Size:       1632     231
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     839    1863 
   Size:        839    1024
 
PshAI GACnn'nnGTC

Cuts at:      0    1813    1863 
   Size:       1813      50
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1344    1376    1863 
   Size:       1344      32     487

  Fragments arranged by size: 

                1344     487      32
 
PstI C_TGCA'G

Cuts at:      0     148     987    1097    1863 
   Size:        148     839     110     766

  Fragments arranged by size: 

                 839     766     148     110
 
PvuII CAG'CTG

Cuts at:      0     814    1863 
   Size:        814    1049
 
SapI GCTCTTCn'nnn_

Cuts at:      0     130    1434    1863 
   Size:        130    1304     429

  Fragments arranged by size: 

                1304     429     130
 
ScaI AGT'ACT

Cuts at:      0    1687    1776    1863 
   Size:       1687      89      87

  Fragments arranged by size: 

                1687      89      87
 
SfcI C'TryA_G

Cuts at:      0     144     272     983    1093    1370    1863 
   Size:        144     128     711     110     277     493

  Fragments arranged by size: 

                 711     493     277     144     128     110
 
SmlI C'TyrA_G

Cuts at:      0     662    1863 
   Size:        662    1201
 
SspI AAT'ATT

Cuts at:      0     786    1193    1863 
   Size:        786     407     670

  Fragments arranged by size: 

                 786     670     407
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1299    1863 
   Size:       1299     564
 
TatI w'GTAC_w

Cuts at:      0    1084    1321    1685    1774    1863 
   Size:       1084     237     364      89      89

  Fragments arranged by size: 

                1084     364     237      89      89
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      75     107     325     357    1863 
   Size:         75      32     218      32    1506

  Fragments arranged by size: 

                1506     218      75      32      32
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     BanI     BbsI    BciVI     BclI    BfrBI
     BglI Bme1580I     BmrI    BpuEI     BsaI    BsaBI    BsaHI     BsgI
  BsiHKAI Bsp1286I    BsrBI    BsrGI    BssSI    BstBI    BstXI    BstYI
     BtgI     BtsI     EaeI     EarI     EciI  Eco57MI    EcoRV     FalI
    HaeII    Hin4I     MslI   MspA1I     NsiI     NspI    PflMI    PshAI
     PsrI     PstI    PvuII     SapI     ScaI     SfcI     SmlI     SspI
    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
    BbvCI     BcgI    BglII     BlpI    BmgBI     BmtI     BplI     BpmI
   Bpu10I    BsaAI    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrFI   BssHII
   BstAPI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI  EcoICRI    EcoNI EcoO109I    EcoRI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI
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