Restriction map for pF1KB5982
 (Linear) (Six-base) MAPSORT of: KIBB5982.seq  Check: 3848  from: 1  to: 1704



KIBB5982.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     509     596    1704 
   Size:        322     187      87    1108

  Fragments arranged by size: 

                1108     322     187      87
 
AfeI AGC'GCT

Cuts at:      0    1192    1669    1704 
   Size:       1192     477      35

  Fragments arranged by size: 

                1192     477      35
 
AflIII A'CryG_T

Cuts at:      0     288    1704 
   Size:        288    1416
 
AleI CACnn'nnGTG

Cuts at:      0     437    1704 
   Size:        437    1267
 
ApoI r'AATT_y

Cuts at:      0     254     560    1320    1704 
   Size:        254     306     760     384

  Fragments arranged by size: 

                 760     384     306     254
 
AvaI C'yCGr_G

Cuts at:      0    1244    1704 
   Size:       1244     460
 
AvrII C'CTAG_G

Cuts at:      0     828    1704 
   Size:        828     876
 
BanI G'GyrC_C

Cuts at:      0    1252    1704 
   Size:       1252     452
 
BanII G_rGCy'C

Cuts at:      0     197     825    1704 
   Size:        197     628     879

  Fragments arranged by size: 

                 879     628     197
 
BbvCI CC'TCA_GC

Cuts at:      0     461    1704 
   Size:        461    1243
 
BlpI GC'TnA_GC

Cuts at:      0     713    1704 
   Size:        713     991
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     615    1704 
   Size:        615    1089
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1136    1172    1704 
   Size:       1136      36     532

  Fragments arranged by size: 

                1136     532      36
 
Bpu10I CC'TnA_GC

Cuts at:      0     461    1704 
   Size:        461    1243
 
BsaHI Gr'CG_yC

Cuts at:      0    1173    1704 
   Size:       1173     531
 
BsaWI w'CCGG_w

Cuts at:      0     986    1704 
   Size:        986     718
 
BseYI C'CCAG_C

Cuts at:      0    1224    1637    1664    1704 
   Size:       1224     413      27      40

  Fragments arranged by size: 

                1224     413      40      27
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1415    1704 
   Size:       1415     289
 
BsiHKAI G_wGCw'C

Cuts at:      0     197    1704 
   Size:        197    1507
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     948    1704 
   Size:        948     756
 
Bsp1286I G_dGCh'C

Cuts at:      0     197     825    1704 
   Size:        197     628     879

  Fragments arranged by size: 

                 879     628     197
 
BspHI T'CATG_A

Cuts at:      0     981    1704 
   Size:        981     723
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     659    1704 
   Size:        659    1045
 
BsrBI CCG'CTC

Cuts at:      0     630    1704 
   Size:        630    1074
 
BsrGI T'GTAC_A

Cuts at:      0     272     306    1704 
   Size:        272      34    1398

  Fragments arranged by size: 

                1398     272      34
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     891    1128    1704 
   Size:        891     237     576

  Fragments arranged by size: 

                 891     576     237
 
BstYI r'GATC_y

Cuts at:      0     747     989    1704 
   Size:        747     242     715

  Fragments arranged by size: 

                 747     715     242
 
Bsu36I CC'TnA_GG

Cuts at:      0      76    1704 
   Size:         76    1628
 
BtgI C'CryG_G

Cuts at:      0     248    1655    1704 
   Size:        248    1407      49

  Fragments arranged by size: 

                1407     248      49
 
EaeI y'GGCC_r

Cuts at:      0    1014    1268    1376    1704 
   Size:       1014     254     108     328

  Fragments arranged by size: 

                1014     328     254     108
 
EarI CTCTTCn'nnn_

Cuts at:      0      76     500     572    1704 
   Size:         76     424      72    1132

  Fragments arranged by size: 

                1132     424      76      72
 
EcoICRI GAG'CTC

Cuts at:      0     195    1704 
   Size:        195    1509
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     509     596    1136    1172    1704 
   Size:        322     187      87     540      36     532

  Fragments arranged by size: 

                 540     532     322     187      87      36
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1346    1704 
   Size:       1346     358
 
EcoO109I rG'GnC_Cy

Cuts at:      0     808    1704 
   Size:        808     896
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     334    1704 
   Size:        334    1370
 
HaeII r_GCGC'y

Cuts at:      0    1194    1671    1704 
   Size:       1194     477      33

  Fragments arranged by size: 

                1194     477      33
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      24      56     807     839    1124    1156    1272    1304 
   Size:         24      32     751      32     285      32     116      32

Cuts at:   1304    1704 
   Size:        400

  Fragments arranged by size: 

                 751     400     285     116      32      32      32      32
                  24
 
HincII GTy'rAC

Cuts at:      0     222     444    1704 
   Size:        222     222    1260

  Fragments arranged by size: 

                1260     222     222
 
MfeI C'AATT_G

Cuts at:      0     435    1571    1704 
   Size:        435    1136     133

  Fragments arranged by size: 

                1136     435     133
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     368    1125    1704 
   Size:        368     757     579

  Fragments arranged by size: 

                 757     579     368
 
MscI TGG'CCA

Cuts at:      0    1270    1378    1704 
   Size:       1270     108     326

  Fragments arranged by size: 

                1270     326     108
 
MslI CAynn'nnrTG

Cuts at:      0     437     889    1183    1424    1513    1704 
   Size:        437     452     294     241      89     191

  Fragments arranged by size: 

                 452     437     294     241     191      89
 
MspA1I CmG'CkG

Cuts at:      0     460     728    1637    1704 
   Size:        460     268     909      67

  Fragments arranged by size: 

                 909     460     268      67
 
NcoI C'CATG_G

Cuts at:      0     248    1655    1704 
   Size:        248    1407      49

  Fragments arranged by size: 

                1407     248      49
 
NspI r_CATG'y

Cuts at:      0     292     619    1224    1704 
   Size:        292     327     605     480

  Fragments arranged by size: 

                 605     480     327     292
 
PasI CC'CwG_GG

Cuts at:      0    1128    1704 
   Size:       1128     576
 
PciI A'CATG_T

Cuts at:      0     288    1704 
   Size:        288    1416
 
PfoI T'CCnGG_A

Cuts at:      0    1495    1704 
   Size:       1495     209
 
PpuMI rG'GwC_Cy

Cuts at:      0     808    1704 
   Size:        808     896
 
PshAI GACnn'nnGTC

Cuts at:      0    1504    1704 
   Size:       1504     200
 
PstI C_TGCA'G

Cuts at:      0     327    1704 
   Size:        327    1377
 
PvuII CAG'CTG

Cuts at:      0     460    1637    1704 
   Size:        460    1177      67

  Fragments arranged by size: 

                1177     460      67
 
SacI G_AGCT'C

Cuts at:      0     197    1704 
   Size:        197    1507
 
SanDI GG'GwC_CC

Cuts at:      0     808    1704 
   Size:        808     896
 
SapI GCTCTTCn'nnn_

Cuts at:      0      76     572    1704 
   Size:         76     496    1132

  Fragments arranged by size: 

                1132     496      76
 
SfcI C'TryA_G

Cuts at:      0     159     323     655     729    1704 
   Size:        159     164     332      74     975

  Fragments arranged by size: 

                 975     332     164     159      74
 
SmaI CCC'GGG

Cuts at:      0    1246    1704 
   Size:       1246     458
 
SpeI A'CTAG_T

Cuts at:      0     187    1704 
   Size:        187    1517
 
SphI G_CATG'C

Cuts at:      0     619    1224    1704 
   Size:        619     605     480

  Fragments arranged by size: 

                 619     605     480
 
StuI AGG'CCT

Cuts at:      0     783    1704 
   Size:        783     921
 
StyI C'CwwG_G

Cuts at:      0     228     248     828    1655    1704 
   Size:        228      20     580     827      49

  Fragments arranged by size: 

                 827     580     228      49      20
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     597    1704 
   Size:        597    1107
 
TatI w'GTAC_w

Cuts at:      0     272     306    1704 
   Size:        272      34    1398

  Fragments arranged by size: 

                1398     272      34
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1250    1282    1447    1479    1704 
   Size:       1250      32     165      32     225

  Fragments arranged by size: 

                1250     225     165      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1106    1704 
   Size:       1106     598
 
XbaI T'CTAG_A

Cuts at:      0    1044    1704 
   Size:       1044     660
 
XmaI C'CCGG_G

Cuts at:      0    1244    1704 
   Size:       1244     460
 
XmnI GAAnn'nnTTC

Cuts at:      0     127     317    1704 
   Size:        127     190    1387

  Fragments arranged by size: 

                1387     190     127
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII     AleI     ApoI     AvaI    AvrII     BanI
    BanII    BbvCI     BlpI     BmrI     BpmI   Bpu10I    BsaHI    BsaWI
    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI
    BsrGI    BstXI    BstYI   Bsu36I     BtgI     EaeI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I   HincII     MfeI
     MmeI     MscI     MslI   MspA1I     NcoI     NspI     PasI     PciI
     PfoI    PpuMI    PshAI     PstI    PvuII     SacI    SanDI     SapI
     SfcI     SmaI     SpeI     SphI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     BaeI    BamHI     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII Bme1580I    BmgBI     BmtI     BplI    BpuEI     BsaI
    BsaAI    BsaBI    BsaXI    BseRI    BsiEI    BsiWI     BsmI    BspEI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI
     KasI     KpnI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI    PflMI     PmeI     PmlI     PpiI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SrfI     SspI     SwaI     XcmI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com