Restriction map for pF1KB5981
 (Linear) (Six-base) MAPSORT of: KIBB5981.seq  Check: 7711  from: 1  to: 1383



KIBB5981.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AccI GT'mk_AC

Cuts at:      0     633    1383 
   Size:        633     750
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      93     231     678    1098    1182    1266    1346    1383 
   Size:         93     138     447     420      84      84      80      37

  Fragments arranged by size: 

                 447     420     138      93      84      84      80      37
            
 
AflIII A'CryG_T

Cuts at:      0     418     554    1383 
   Size:        418     136     829

  Fragments arranged by size: 

                 829     418     136
 
ApoI r'AATT_y

Cuts at:      0     170    1148    1316    1383 
   Size:        170     978     168      67

  Fragments arranged by size: 

                 978     170     168      67
 
BamHI G'GATC_C

Cuts at:      0      63    1383 
   Size:         63    1320
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     149     164     555     975    1383 
   Size:        149      15     391     420     408

  Fragments arranged by size: 

                 420     408     391     149      15
 
BmgBI CAC'GTC

Cuts at:      0     376    1383 
   Size:        376    1007
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     614    1034    1366    1383 
   Size:        614     420     332      17

  Fragments arranged by size: 

                 614     420     332      17
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     308     506     674     758    1094    1178    1262 
   Size:         64     244     198     168      84     336      84      84

Cuts at:   1262    1346    1383 
   Size:         84      37

  Fragments arranged by size: 

                 336     244     198     168      84      84      84      84
                  64      37
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     462    1383 
   Size:        462     921
 
BsaAI yAC'GTr

Cuts at:      0     421     469    1383 
   Size:        421      48     914

  Fragments arranged by size: 

                 914     421      48
 
BsaWI w'CCGG_w

Cuts at:      0     904    1383 
   Size:        904     479
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      61    1383 
   Size:         61    1322
 
BsmI GAATG_Cn'

Cuts at:      0     542     676     760     937    1383 
   Size:        542     134      84     177     446

  Fragments arranged by size: 

                 542     446     177     134      84
 
BsrDI GCAATG_nn'

Cuts at:      0     685     999    1105    1273    1383 
   Size:        685     314     106     168     110

  Fragments arranged by size: 

                 685     314     168     110     106
 
BsrGI T'GTAC_A

Cuts at:      0    1177    1383 
   Size:       1177     206
 
BssSI C'ACGA_G

Cuts at:      0     889    1383 
   Size:        889     494
 
BstBI TT'CG_AA

Cuts at:      0     546     798     882    1050    1134    1383 
   Size:        546     252      84     168      84     249

  Fragments arranged by size: 

                 546     252     249     168      84      84
 
BstYI r'GATC_y

Cuts at:      0      63    1383 
   Size:         63    1320
 
BstZ17I GTA'TAC

Cuts at:      0     634    1383 
   Size:        634     749
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     436    1383 
   Size:        436     947
 
BtsI GCAGTG_nn'

Cuts at:      0     315     769    1383 
   Size:        315     454     614

  Fragments arranged by size: 

                 614     454     315
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     663     698     747     782    1383 
   Size:        663      35      49      35     601

  Fragments arranged by size: 

                 663     601      49      35      35
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64      93     231     308     506     674     678     758 
   Size:         64      29     138      77     198     168       4      80

Cuts at:    758    1094    1098    1178    1182    1262    1266    1346    1383 
   Size:        336       4      80       4      80       4      80      37

  Fragments arranged by size: 

                 336     198     168     138      80      80      80      80
                  77      64      37      29       4       4       4       4
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     334    1064    1383 
   Size:        334     730     319

  Fragments arranged by size: 

                 730     334     319
 
EcoRI G'AATT_C

Cuts at:      0    1148    1383 
   Size:       1148     235
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     435     467    1383 
   Size:        435      32     916

  Fragments arranged by size: 

                 916     435      32
 
HincII GTy'rAC

Cuts at:      0     560    1383 
   Size:        560     823
 
HindIII A'AGCT_T

Cuts at:      0     773    1383 
   Size:        773     610
 
HpaI GTT'AAC

Cuts at:      0     560    1383 
   Size:        560     823
 
NspI r_CATG'y

Cuts at:      0     558    1383 
   Size:        558     825
 
PciI A'CATG_T

Cuts at:      0     554    1383 
   Size:        554     829
 
PfoI T'CCnGG_A

Cuts at:      0     285     483    1383 
   Size:        285     198     900

  Fragments arranged by size: 

                 900     285     198
 
PpuMI rG'GwC_Cy

Cuts at:      0     334    1064    1383 
   Size:        334     730     319

  Fragments arranged by size: 

                 730     334     319
 
PsiI TTA'TAA

Cuts at:      0    1004    1383 
   Size:       1004     379
 
SfcI C'TryA_G

Cuts at:      0      84     125    1383 
   Size:         84      41    1258

  Fragments arranged by size: 

                1258      84      41
 
SmlI C'TyrA_G

Cuts at:      0     441    1383 
   Size:        441     942
 
TatI w'GTAC_w

Cuts at:      0     791    1177    1305    1383 
   Size:        791     386     128      78

  Fragments arranged by size: 

                 791     386     128      78
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     259    1383 
   Size:        259    1124
 
XmnI GAAnn'nnTTC

Cuts at:      0     691     775    1111    1148    1195    1279    1316    1383 
   Size:        691      84     336      37      47      84      37      67

  Fragments arranged by size: 

                 691     336      84      84      67      47      37      37
            
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     ApoI    BamHI     BbsI    BmgBI     BmrI
     BpmI    BpuEI    BsaAI    BsaWI    BseRI     BsmI    BsrDI    BsrGI
    BssSI    BstBI    BstYI  BstZ17I    BtgZI     BtsI    CspCI  Eco57MI
 EcoO109I    EcoRI    Hin4I   HincII  HindIII     HpaI     NspI     PciI
     PfoI    PpuMI     PsiI     SfcI     SmlI     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
     BmtI     BplI   Bpu10I     BsaI    BsaBI    BsaHI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrFI   BssHII   BstAPI   BstEII    BstXI   Bsu36I
     BtgI     ClaI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PmeI     PmlI     PpiI
    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I
     XbaI     XhoI     XmaI     ZraI
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