Restriction map for pF1KB5959
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

Acc65I      AccI        AccII       AclI        AcsI        
AgeI        AhdI        ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BglI        
BglII       BlnI        BpuAI       BsaAI       BsaBI       
BsiWI       BsmI        BspHI       BspLU11I    BsrDI       
BssHII      BssSI       Bst1107I    BstBI       BstUI       
BstZ17I     DraI        DraIII      EagI        Eam1105I    
EclXI       Eco52I      EcoRI       EcoRV       FseI        
FspI        HincII      HindII      HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MunI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        Tsp509I     
Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AatII         522  GACGTC               GACGT^C
AccIII       1328  TCCGGA               T^CCGGA
AflII         184  CTTAAG               C^TTAAG
AflIII        897  ACACGT               A^C[GA][CT]GT
AocI         1166  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      1526  AGCGCT               AGC^GCT
BalI          850  TGGCCA               TGG^CCA
BfaI          112  CTAG                 C^TAG
BfrI          184  CTTAAG               C^TTAAG
BlpI         1341  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     1341  GCTAAGC              GC^T[ACGT]AGC
BsaI          766  GAGACC               GGTCTC(1/5)
BseAI        1328  TCCGGA               T^CCGGA
BsiEI        1336  CGGTCG               CG[GA][CT]^CG
BsmBI         444  CGTCTC               CGTCTC(1/5)
BspDI         457  ATCGAT               AT^CGAT
BspEI        1328  TCCGGA               T^CCGGA
BsrBI         122  CCGCTC               CCGCTC(-3/-3)
BstEII        309  GGTGACC              G^GT[ACGT]ACC
BstPI         309  GGTGACC              G^GT[ACGT]ACC
BstXI         716  CCAAGTATTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1250  AGATCC               [GA]^GATC[CT]
Bsu36I       1166  CCTCAGG              CC^T[ACGT]AGG
CelII        1341  GCTAAGC              GC^T[ACGT]AGC
ClaI          457  ATCGAT               AT^CGAT
CpoI         1325  CGGTCCG              CG^G[AT]CCG
DrdI          522  GACGTCCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1097  CTCTTC               CTCTTC(1/4)
Eco47III     1526  AGCGCT               AGC^GCT
Eco81I       1166  CCTCAGG              CC^T[ACGT]AGG
EcoNI         207  CCTTCAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       309  GGTGACC              G^GT[ACGT]ACC
EcoT22I      1223  ATGCAT               ATGCA^T
FauI          948  GCGGG                CCCGC(4/6)
HaeII        1526  AGCGCT               [GA]GCGC^[CT]
HindIII      1344  AAGCTT               A^AGCTT
Ksp632I      1097  CTCTTC               CTCTTC(1/4)
MaeI          112  CTAG                 C^TAG
MflI         1250  AGATCC               [GA]^GATC[CT]
MluNI         850  TGGCCA               TGG^CCA
MroI         1328  TCCGGA               T^CCGGA
MscI          850  TGGCCA               TGG^CCA
MseI          185  TTAA                 T^TAA
NsiI         1223  ATGCAT               ATGCA^T
RsrII        1325  CGGTCCG              CG^G[AT]CCG
SapI         1096  GCTCTTC              GCTCTTC(1/4)
SmlI          184  CTTAAG               C^T[CT][GA]AG
StuI         1517  AGGCCT               AGG^CCT
TfiI          991  GAATC                G^A[AT]TC
Tru9I         185  TTAA                 T^TAA
XcmI          747  CCAAACTGATCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1250  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcyI           20  GACGCC               G[GA]^CG[CT]C
              522  GACGTC              
Alw44I        267  GTGCAC               G^TGCAC
             1494  GTGCAC              
AlwI          772  GATCC                GGATC(4/5)
             1251  GATCC               
ApaLI         267  GTGCAC               G^TGCAC
             1494  GTGCAC              
BclI          753  TGATCA               T^GATCA
              936  TGATCA              
BsaHI          20  GACGCC               G[GA]^CG[CT]C
              522  GACGTC              
BsaWI        1323  TCCGGT               [AT]^CCGG[AT]
             1328  TCCGGA              
BseRI         358  GAGGAG               GAGGAG(10/8)
              952  GAGGAG              
BsgI         1111  CTGCAC               GTGCAG(16/14)
              976  GTGCAG              
Bsp1407I      546  TGTACA               T^GTACA
              836  TGTACA              
BsrFI        1334  GCCGGT               [GA]^CCGG[CT]
             1505  GCCGGT              
BsrGI         546  TGTACA               T^GTACA
              836  TGTACA              
CfoI          873  GCGC                 GCG^C
             1527  GCGC                
Cfr10I       1334  GCCGGT               [GA]^CCGG[CT]
             1505  GCCGGT              
DsaI          512  CCATGG               C^C[GA][CT]GG
              604  CCATGG              
EaeI          509  CGGCCA               [CT]^GGCC[GA]
              850  TGGCCA              
FbaI          753  TGATCA               T^GATCA
              936  TGATCA              
HgaI           20  GACGC                GACGC(5/10)
              481  GACGC               
HhaI          873  GCGC                 GCG^C
             1527  GCGC                
Hin1I          20  GACGCC               G[GA]^CG[CT]C
              522  GACGTC              
HinP1I        873  GCGC                 G^CGC
             1527  GCGC                
MaeII         523  ACGT                 A^CGT
              899  ACGT                
MboII        1512  GAAGA                GAAGA(8/7)
             1098  TCTTC               
MslI          658  CACTGGAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              969  CACAGAGGTG          
NcoI          512  CCATGG               C^CATGG
              604  CCATGG              
PpuMI          60  GGGACCC              [GA]G^G[AT]CC[CT]
             1195  GGGTCCT             
SspBI         546  TGTACA               T^GTACA
              836  TGTACA              
TaqI          458  TCGA                 T^CGA
             1475  TCGA                
TthHB8I       458  TCGA                 T^CGA
             1475  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       ApaI        
AspHI       AvaI        AvaII       BanI        BanII       
BbvI        BcnI        BmyI        BpmI        BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MnlI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        StyI        TseI        Tsp45I      TspRI       
Van91I      XmaCI       XmaI        
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