Restriction map for pF1KB5927
##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspHI       AspI        AvaI        AviII       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglII       BsaHI       
BsaWI       BseAI       BsiHKAI     BsiWI       BsmBI       
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrBI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
CpoI        DraI        DraIII      DrdI        Eco47III    
EcoO65I     EcoRI       FbaI        FspI        HaeII       
Hin1I       HindIII     HpaI        KasI        KpnI        
MfeI        MluI        MroI        MunI        NarI        
NheI        NotI        NruI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RsrII       SacI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AhdI         1685  GACCCACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         363  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1482  CCTAAGG              CC^T[ACGT]AGG
ApaI          740  GGGCCC               GGGCC^C
AspEI        1685  GACCCACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII         903  CCTAGG               C^CTAGG
BglI          908  GCCAAAATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          903  CCTAGG               C^CTAGG
BlpI         1620  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1620  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1441  TACGTG               [CT]AC^GT[GA]
BsaBI         592  GATATCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI          971  GCATTC               GAATGC(1/-1)
BstAPI        871  GCATTTGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I       1482  CCTAAGG              CC^T[ACGT]AGG
CelII        1620  GCTGAGC              GC^T[ACGT]AGC
Eam1105I     1685  GACCCACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1482  CCTAAGG              CC^T[ACGT]AGG
EcoNI          96  CCTCTTCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         592  GATATC               GAT^ATC
EcoT22I      1502  ATGCAT               ATGCA^T
FseI           59  GGCCGGCC             GGCCGG^CC
HgaI          682  GACGC                GACGC(5/10)
HincII        297  GTCGAC               GT[CT]^[GA]AC
HindII        297  GTCGAC               GT[CT]^[GA]AC
HphI         1697  TCACC                GGTGA(8/7)
MamI          592  GATATCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI          182  CCATGG               C^CATGG
NdeI          815  CATATG               CA^TATG
NsiI         1502  ATGCAT               ATGCA^T
SalI          297  GTCGAC               G^TCGAC
SfcI         1410  CTACAG               C^T[GA][CT]AG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccI          297  GTCGAC               GT^[AC][GT]AC
             1408  GTCTAC              
AfaI          207  GTAC                 GT^AC
             1563  GTAC                
BamHI        1314  GGATCC               G^GATCC
             1584  GGATCC              
BcnI           18  CCCGG                CC^[GC]GG
             1226  CCCGG               
BsaI         1075  GAGACC               GGTCTC(1/5)
              309  GGTCTC              
BseRI         848  GAGGAG               GAGGAG(10/8)
             1011  GAGGAG              
BspHI        1337  TCATGA               T^CATGA
             1662  TCATGA              
BstYI        1314  GGATCC               [GA]^GATC[CT]
             1584  GGATCC              
EagI           58  CGGCCG               C^GGCCG
               62  CGGCCG              
EclXI          58  CGGCCG               C^GGCCG
               62  CGGCCG              
Eco52I         58  CGGCCG               C^GGCCG
               62  CGGCCG              
EcoT14I       182  CCATGG               C^C[AT][AT]GG
              903  CCTAGG              
KspI          248  CCGCGG               CCGC^GG
              999  CCGCGG              
MaeII        1122  ACGT                 A^CGT
             1442  ACGT                
MaeIII       1360  GTCAC                ^GT[ACGT]AC
             1465  GTGAC               
MflI         1314  GGATCC               [GA]^GATC[CT]
             1584  GGATCC              
MseI          407  TTAA                 T^TAA
              602  TTAA                
NaeI           60  GCCGGC               GCC^GGC
               64  GCCGGC              
NciI           18  CCCGG                CC^[GC]GG
             1226  CCCGG               
NgoMIV         60  GCCGGC               G^CCGGC
               64  GCCGGC              
PvuII         338  CAGCTG               CAG^CTG
             1082  CAGCTG              
RcaI         1337  TCATGA               T^CATGA
             1662  TCATGA              
RsaI          207  GTAC                 GT^AC
             1563  GTAC                
SacII         248  CCGCGG               CCGC^GG
              999  CCGCGG              
StyI          182  CCATGG               C^C[AT][AT]GG
              903  CCTAGG              
Tru9I         407  TTAA                 T^TAA
              602  TTAA                
Tsp45I       1360  GTCAC                ^GT[GC]AC
             1465  GTGAC               
XcmI          224  CCATCGAGATCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1025  CCAGCATGGCCCTGG     
XhoII        1314  GGATCC               [GA]^GATC[CT]
             1584  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AccII       AciI        AcsI        AluI        AlwI        
ApoI        AvaII       BalI        BanII       BbsI        
BbvI        BfaI        BmyI        BpmI        BpuAI       
BsaJI       BsgI        BsiEI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrDI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       ItaI        Ksp632I     
MaeI        MboI        MboII       MluNI       MnlI        
MscI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NdeII       NlaIII      NlaIV       
PleI        PpuMI       Sau3AI      Sau96I      ScrFI       
SfaNI       TaqI        TfiI        TseI        Tsp509I     
TspRI       TthHB8I     
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