Restriction map for pF1KB5926
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BalI        
BbrPI       BcgI        BciVI       BclI        BfrI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BseAI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspLU11I    BsrGI       BssHII      
BssSI       Bst1107I    BstEII      BstPI       BstZ17I     
CelII       ClaI        CpoI        DraI        DraIII      
Eam1105I    Eco47III    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        MaeII       
MamI        MfeI        MluI        MluNI       MroI        
MscI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspI        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RsaI        RsrII       SacI        
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     XbaI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AcyI           48  GGCGCC               G[GA]^CG[CT]C
ApaI         1602  GGGCCC               GGGCC^C
AvaI          190  CTCGAG               C^[CT]CG[GA]G
BamHI        1112  GGATCC               G^GATCC
BanI           48  GGCGCC               G^G[CT][GA]CC
BbeI           48  GGCGCC               GGCGC^C
BbsI          885  GAAGAC               GAAGAC(2/6)
BglI          515  GCCTGGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1319  AGATCT               A^GATCT
BpuAI         885  GAAGAC               GAAGAC(2/6)
BsaHI          48  GGCGCC               G[GA]^CG[CT]C
BsaWI           9  ACCGGA               [AT]^CCGG[AT]
BsoBI         190  CTCGAG               C^[CT]CG[GA]G
BspHI         684  TCATGA               T^CATGA
BspMI        1380  GCAGGT               ACCTGC(4/8)
BsrBI        1375  GAGCGG               CCGCTC(-3/-3)
BsrDI        1073  GCAATG               GCAATG(2/0)
BstBI         795  TTCGAA               TT^CGAA
DrdI          757  GACCAGCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          234  CGGCCG               C^GGCCG
EclXI         234  CGGCCG               C^GGCCG
Eco52I        234  CGGCCG               C^GGCCG
HgaI           13  GACGC                GACGC(5/10)
Hin1I          48  GGCGCC               G[GA]^CG[CT]C
HphI         1054  TCACC                GGTGA(8/7)
KasI           48  GGCGCC               G^GCGCC
KspI          156  CCGCGG               CCGC^GG
MslI         1452  CACCTCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI           45  GCCGGC               GCC^GGC
NarI           48  GGCGCC               GG^CGCC
NgoMIV         45  GCCGGC               G^CCGGC
NspV          795  TTCGAA               TT^CGAA
PaeR7I        190  CTCGAG               C^TCGAG
PflMI        1521  CCAGAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI          684  TCATGA               T^CATGA
SacII         156  CCGCGG               CCGC^GG
SfoI           48  GGCGCC               GGC^GCC
SfuI          795  TTCGAA               TT^CGAA
SmlI          190  CTCGAG               C^T[CT][GA]AG
Tsp509I       413  AATT                 ^AATT
Van91I       1521  CCAGAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1448  CCACCACCTCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          190  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AlwI         1113  GATCC                GGATC(4/5)
             1112  GGATC               
AspHI         136  GTGCTC               G[AT]GC[AT]^C
             1216  GTGCTC              
BsgI         1032  CTGCAC               GTGCAG(16/14)
              220  GTGCAG              
BsiEI         215  CGGTCG               CG[GA][CT]^CG
              234  CGGCCG              
BsiHKAI       136  GTGCTC               G[AT]GC[AT]^C
             1216  GTGCTC              
BsrFI          45  GCCGGC               [GA]^CCGG[CT]
              213  GCCGGT              
BstAPI         90  GCAGGCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              873  GCAGCAGCTGC         
BstF5I        804  GGATG                GGATG(2/0)
              918  GGATG               
BstYI        1112  GGATCC               [GA]^GATC[CT]
             1319  AGATCT              
Cfr10I         45  GCCGGC               [GA]^CCGG[CT]
              213  GCCGGT              
EcoNI          84  CCTGCTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1562  CCTTCCTGAGG         
FokI          804  GGATG                GGATG(9/13)
              918  GGATG               
MflI         1112  GGATCC               [GA]^GATC[CT]
             1319  AGATCT              
NcoI          446  CCATGG               C^CATGG
             1457  CCATGG              
TfiI          718  GAATC                G^A[AT]TC
              798  GAATC               
Tsp45I        711  GTCAC                ^GT[GC]AC
             1365  GTCAC               
XhoII        1112  GGATCC               [GA]^GATC[CT]
             1319  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AccII       AciI        AluI        AlwNI       AocI        
AvaII       BanII       BbvCI       BbvI        BcnI        
BfaI        BmyI        BpmI        BsaI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstNI       BstUI       
BstXI       Bsu36I      Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        Eco81I      EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MnlI        MseI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       PshAI       PstI        
PvuII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        Tru9I       TseI        
TspRI       TthHB8I     
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