Restriction map for pF1KB5908
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflIII      AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaII       AviII       AvrII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BseAI       BsiEI       
BsiWI       BsmI        BspEI       BspLU11I    BsrBI       
BsrDI       BssHII      Bst1107I    BstEII      BstPI       
BstZ17I     Bsu36I      CelII       CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       Hin1I       HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MroI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PflFI       PflMI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        Tsp509I     
Tth111I     Van91I      XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccII        1090  CGCG                 CG^CG
AflII         349  CTTAAG               C^TTAAG
AgeI          510  ACCGGT               A^CCGGT
Asp700I      1255  GAACCCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          899  TGGCCA               TGG^CCA
BbsI         1426  GAAGAC               GAAGAC(2/6)
BfrI          349  CTTAAG               C^TTAAG
BpuAI        1426  GAAGAC               GAAGAC(2/6)
BsaI         1213  GGTCTC               GGTCTC(1/5)
BsaWI         510  ACCGGT               [AT]^CCGG[AT]
BsgI          355  GTGCAG               GTGCAG(16/14)
BsmBI        1543  GAGACG               CGTCTC(1/5)
Bsp1407I     1312  TGTACA               T^GTACA
BspDI         679  ATCGAT               AT^CGAT
BspHI          51  TCATGA               T^CATGA
BsrGI        1312  TGTACA               T^GTACA
BssSI         647  CACGAG               CACGAG(-5/-1)
BstBI          31  TTCGAA               TT^CGAA
BstUI        1090  CGCG                 CG^CG
BstXI        1403  CCACAAAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI          679  ATCGAT               AT^CGAT
DraII         124  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EaeI          899  TGGCCA               [CT]^GGCC[GA]
EcoO109I      124  GGGGCCT              [GA]G^G[ACGT]CC[CT]
HgaI          652  GCGTC                GACGC(5/10)
HincII         86  GTCAAC               GT[CT]^[GA]AC
HindII         86  GTCAAC               GT[CT]^[GA]AC
MaeIII       1465  GTCAC                ^GT[ACGT]AC
MluNI         899  TGGCCA               TGG^CCA
MscI          899  TGGCCA               TGG^CCA
MspA1I         91  CCGCTG               C[AC]G^C[GT]G
MvnI         1090  CGCG                 CG^CG
NcoI          542  CCATGG               C^CATGG
NspV           31  TTCGAA               TT^CGAA
PaeR7I       1178  CTCGAG               C^TCGAG
PinAI         510  ACCGGT               A^CCGGT
RcaI           51  TCATGA               T^CATGA
SfuI           31  TTCGAA               TT^CGAA
SgrAI         509  CACCGGTG             C[GA]^CCGG[CT]G
SspBI        1312  TGTACA               T^GTACA
StuI          819  AGGCCT               AGG^CCT
Tsp45I       1465  GTCAC                ^GT[GC]AC
XhoI         1178  CTCGAG               C^TCGAG
XmnI         1255  GAACCCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AlwNI         232  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              878  CAGAGTCTG           
AvaI          771  CTCGGG               C^[CT]CG[GA]G
             1178  CTCGAG              
BamHI         735  GGATCC               G^GATCC
             1414  GGATCC              
BcnI          517  CCGGG                CC^[GC]GG
             1495  CCCGG               
BfaI          697  CTAG                 C^TAG
              982  CTAG                
BsoBI         771  CTCGGG               C^[CT]CG[GA]G
             1178  CTCGAG              
BspMI         330  GCAGGT               ACCTGC(4/8)
              411  GCAGGT              
BsrFI         510  ACCGGT               [GA]^CCGG[CT]
             1136  ACCGGC              
BstYI         735  GGATCC               [GA]^GATC[CT]
             1414  GGATCC              
Cfr10I        510  ACCGGT               [GA]^CCGG[CT]
             1136  ACCGGC              
Cfr13I        125  GGGCC                G^G[ACGT]CC
              246  GGCCC               
EcoT14I       542  CCATGG               C^C[AT][AT]GG
              801  CCAAGG              
MaeI          697  CTAG                 C^TAG
              982  CTAG                
MaeII         296  ACGT                 A^CGT
              760  ACGT                
MflI          735  GGATCC               [GA]^GATC[CT]
             1414  GGATCC              
NciI          517  CCGGG                CC^[GC]GG
             1495  CCCGG               
NspI          450  ACATGC               [GA]CATG^[CT]
             1016  ACATGC              
PleI          276  GAGTC                GAGTC(4/5)
              880  GAGTC               
PstI          238  CTGCAG               CTGCA^G
             1272  CTGCAG              
SacI          871  GAGCTC               GAGCT^C
             1181  GAGCTC              
Sau96I        125  GGGCC                G^G[ACGT]CC
              246  GGCCC               
StyI          542  CCATGG               C^C[AT][AT]GG
              801  CCAAGG              
XcmI          533  CCAACAGCCCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1012  CCAGACATGCGCTGG     
XhoII         735  GGATCC               [GA]^GATC[CT]
             1414  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
BanI        BanII       BbvI        BmyI        BpmI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstAPI      BstF5I      BstNI       Cac8I       CfoI        
DdeI        DpnII       DsaI        EarI        EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MboI        MboII       MnlI        MseI        
MslI        MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       RsaI        SapI        Sau3AI      
ScrFI       SfaNI       SfcI        SmlI        TaqI        
TfiI        Tru9I       TseI        TspRI       TthHB8I     
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