Restriction map for pF1KB5908
 (Circular) (Six-base) MAPSORT of: pF1KB5908.seq  Check: 548  from: 1  to: 4673



pF1KB5908  4673 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    429     429 
   Size:       4673
 
AccI GT'mk_AC

Cuts at:   1711    1711 
   Size:       4673
 
Acc65I G'GTAC_C

Cuts at:   1689    1689 
   Size:       4673
 
AclI AA'CG_TT

Cuts at:   4513    4513 
   Size:       4673
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    223    1013    1381    2498    2930    3677    3945     223 
   Size:        790     368    1117     432     747     268     951

  Fragments arranged by size: 

                1117     951     790     747     432     368     268
 
AflII C'TTAA_G

Cuts at:    457     457 
   Size:       4673
 
AflIII A'CryG_T

Cuts at:   2025    3129    4106    2025 
   Size:       1104     977    2592

  Fragments arranged by size: 

                2592    1104     977
 
AgeI A'CCGG_T

Cuts at:    618    3061     618 
   Size:       2443    2230
 
AleI CACnn'nnGTG

Cuts at:    439    1664     439 
   Size:       1225    3448
 
AlwNI CAG_nnn'CTG

Cuts at:    345     991    1978    3545     345 
   Size:        646     987    1567    1473

  Fragments arranged by size: 

                1567    1473     987     646
 
ApaLI G'TGCA_C

Cuts at:   3443    3443 
   Size:       4673
 
ApoI r'AATT_y

Cuts at:   1677    4026    4282    1677 
   Size:       2349     256    2068

  Fragments arranged by size: 

                2349    2068     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4673
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4673
 
AvaI C'yCGr_G

Cuts at:    879    1286    1693     879 
   Size:        407     407    3859

  Fragments arranged by size: 

                3859     407     407
 
BamHI G'GATC_C

Cuts at:    843    1522    1698     843 
   Size:        679     176    3818

  Fragments arranged by size: 

                3818     679     176
 
BanI G'GyrC_C

Cuts at:    441     621    1447    1689    2103    2351    2386     441 
   Size:        180     826     242     414     248      35    2728

  Fragments arranged by size: 

                2728     826     414     248     242     180      35
 
BanII G_rGCy'C

Cuts at:    170     983    1293    1687    2717     170 
   Size:        813     310     394    1030    2126

  Fragments arranged by size: 

                2126    1030     813     394     310
 
BbeI G_GCGC'C

Cuts at:   2107    2355    2107 
   Size:        248    4425
 
BbsI GAAGACnn'nnnn_

Cuts at:   1541    1541 
   Size:       4673
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    4639
 
BciVI GTATCCnnnnn_n'

Cuts at:   2571    3332    4673    2571 
   Size:        761    1341    2571

  Fragments arranged by size: 

                2571    1341     761
 
BclI T'GATC_A

Cuts at:   2193    2193 
   Size:       4673
 
BglII A'GATC_T

Cuts at:   2188    4225    2188 
   Size:       2037    2636
 
BlpI GC'TnA_GC

Cuts at:   1785    1785 
   Size:       4673
 
Bme1580I G_kGCm'C

Cuts at:    380     416     736    2298    2391    3447     380 
   Size:         36     320    1562      93    1056    1606

  Fragments arranged by size: 

                1606    1562    1056     320      93      36
 
BmrI ACTGGGnnnn_n'

Cuts at:    351    1487    1885    1966    2301    4445     351 
   Size:       1136     398      81     335    2144     579

  Fragments arranged by size: 

                2144    1136     579     398     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3040    3040 
   Size:       4673
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    243    1252    1316     243 
   Size:       1009      64    3600

  Fragments arranged by size: 

                3600    1009      64
 
Bpu10I CC'TnA_GC

Cuts at:    574     574 
   Size:       4673
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1393    1849    2728    3220    3518    1393 
   Size:        456     879     492     298    2548

  Fragments arranged by size: 

                2548     879     492     456     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1327    4377    1327 
   Size:       3050    1623
 
BsaAI yAC'GTr

Cuts at:   2026    2026 
   Size:       4673
 
BsaBI GATnn'nnATC

Cuts at:      6    2187    4224       6 
   Size:       2181    2037     455

  Fragments arranged by size: 

                2181    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   2104    2352    3054    4550    2104 
   Size:        248     702    1496    2227

  Fragments arranged by size: 

                2227    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    618    1862    2383    3061    3335    3482    3893     618 
   Size:       1244     521     678     274     147     411    1398

  Fragments arranged by size: 

                1398    1244     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1545    1575    1620    1650    1545 
   Size:         30      45      30    4568

  Fragments arranged by size: 

                4568      45      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    166     246    1644     166 
   Size:         80    1398    3195

  Fragments arranged by size: 

                3195    1398      80
 
BseYI C'CCAG_C

Cuts at:   2098    2856    3433    3929    2098 
   Size:        758     577     496    2842

  Fragments arranged by size: 

                2842     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    484     484 
   Size:       4673
 
BsiEI CG_ry'CG

Cuts at:    104    2215    2261    3469     104 
   Size:       2111      46    1208    1308

  Fragments arranged by size: 

                2111    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    983    1005    1238    1293    1661    1687    2465    2655    3447 
   Size:         22     233      55     368      26     778     190     792

Cuts at:   3447     983 
   Size:       2209

  Fragments arranged by size: 

                2209     792     778     368     233     190      55      26
                  22
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1645    1645 
   Size:       4673
 
Bsp1286I G_dGCh'C

Cuts at:    170     380     416     736     983    1005    1238    1293    1661 
   Size:        210      36     320     247      22     233      55     368

Cuts at:   1661    1687    2298    2391    2465    2655    2717    3447     170 
   Size:         26     611      93      74     190      62     730    1396

  Fragments arranged by size: 

                1396     730     611     368     320     247     233     210
                 190      93      74      62      55      36      26      22
            
 
BspEI T'CCGG_A

Cuts at:   3893    3893 
   Size:       4673
 
BspHI T'CATG_A

Cuts at:    159    3849     159 
   Size:       3690     983
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    429     510    1723    1937    2239    2620     429 
   Size:         81    1213     214     302     381    2482

  Fragments arranged by size: 

                2482    1213     381     302     214      81
 
BsrBI CCG'CTC

Cuts at:     50    2965    3019    4503    4668      50 
   Size:       2915      54    1484     165      55

  Fragments arranged by size: 

                2915    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2585    2585 
   Size:       4673
 
BsrFI r'CCGG_y

Cuts at:    618    1244    2671    2852    3061     618 
   Size:        626    1427     181     209    2230

  Fragments arranged by size: 

                2230    1427     626     209     181
 
BsrGI T'GTAC_A

Cuts at:   1420    1420 
   Size:       4673
 
BssSI C'ACGA_G

Cuts at:    755    2944    3302     755 
   Size:       2189     358    2126

  Fragments arranged by size: 

                2189    2126     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    297     345    1254    2050     297 
   Size:         48     909     796    2920

  Fragments arranged by size: 

                2920     909     796      48
 
BstBI TT'CG_AA

Cuts at:    140    3034     140 
   Size:       2894    1779
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1518    1518 
   Size:       4673
 
BstYI r'GATC_y

Cuts at:      1     843    1522    1698    2165    2188    2523    2769    3781 
   Size:        842     679     176     467      23     335     246    1012

Cuts at:   3781    3867    3879    4225       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     842     679     467     449     346     335     246
                 176      86      23      12
 
BtgI C'CryG_G

Cuts at:    650    1160    1316     650 
   Size:        510     156    4007

  Fragments arranged by size: 

                4007     510     156
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2623    2804    2967    2623 
   Size:        181     163    4329

  Fragments arranged by size: 

                4329     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1916    1916 
   Size:       4673
 
ClaI AT'CG_AT

Cuts at:    788     788 
   Size:       4673
 
DraI TTT'AAA

Cuts at:   1672    1672 
   Size:       4673
 
DrdI GACnn_nn'nnGTC

Cuts at:   2379    3237    2379 
   Size:        858    3815
 
EaeI y'GGCC_r

Cuts at:   1007    2258    2432    2823    2850    4195    1007 
   Size:       1251     174     391      27    1345    1485

  Fragments arranged by size: 

                1485    1345    1251     391     174      27
 
EagI C'GGCC_G

Cuts at:   2258    2258 
   Size:       4673
 
EarI CTCTTCn'nnn_

Cuts at:    158     199     701    1643    2696    2906     158 
   Size:         41     502     942    1053     210    1925

  Fragments arranged by size: 

                1925    1053     942     502     210      41
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    207    2881    3191    3337    4483     207 
   Size:       2674     310     146    1146     397

  Fragments arranged by size: 

                2674    1146     397     310     146
 
EcoICRI GAG'CTC

Cuts at:    981    1291    1685     981 
   Size:        310     394    3969

  Fragments arranged by size: 

                3969     394     310
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    223     243    1013    1252    1316    1381    2498    2930    3677 
   Size:         20     770     239      64      65    1117     432     747

Cuts at:   3677    3945     223 
   Size:        268     951

  Fragments arranged by size: 

                1117     951     770     747     432     268     239      65
                  64      20
 
EcoO109I rG'GnC_Cy

Cuts at:    233    1812     233 
   Size:       1579    3094
 
EcoRI G'AATT_C

Cuts at:   1677    1677 
   Size:       4673
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1411    2692    1411 
   Size:       1281    3392
 
FspI TGC'GCA

Cuts at:   2454    2454 
   Size:       4673
 
HaeII r_GCGC'y

Cuts at:   2107    2355    3377    4356    2107 
   Size:        248    1022     979    2424

  Fragments arranged by size: 

                2424    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2515    2545    2547    2577    2515 
   Size:         30       2      30    4611

  Fragments arranged by size: 

                4611      30      30       2
 
HincII GTy'rAC

Cuts at:    196    1712     196 
   Size:       1516    3157
 
KasI G'GCGC_C

Cuts at:   2103    2351    2103 
   Size:        248    4425
 
KpnI G_GTAC'C

Cuts at:   1693    1693 
   Size:       4673
 
MluI A'CGCG_T

Cuts at:   4106    4106 
   Size:       4673
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    698    3344    3528     698 
   Size:       2646     184    1843

  Fragments arranged by size: 

                2646    1843     184
 
MscI TGG'CCA

Cuts at:   1009    2434    1009 
   Size:       1425    3248
 
MslI CAynn'nnrTG

Cuts at:    439     655    1516    1543    1664    2662    2789     439 
   Size:        216     861      27     121     998     127    2323

  Fragments arranged by size: 

                2323     998     861     216     127     121      27
 
MspA1I CmG'CkG

Cuts at:    201    1784    2098    2458    3471    3716     201 
   Size:       1583     314     360    1013     245    1158

  Fragments arranged by size: 

                1583    1158    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2854    2854 
   Size:       4673
 
NarI GG'CG_CC

Cuts at:   2104    2352    2104 
   Size:        248    4425
 
NcoI C'CATG_G

Cuts at:    650     650 
   Size:       4673
 
NgoMIV G'CCGG_C

Cuts at:   2852    2852 
   Size:       4673
 
NspI r_CATG'y

Cuts at:    562    1128    1726    2226    3133     562 
   Size:        566     598     500     907    2102

  Fragments arranged by size: 

                2102     907     598     566     500
 
PciI A'CATG_T

Cuts at:   3129    3129 
   Size:       4673
 
PmeI GTTT'AAAC

Cuts at:   1672    1672 
   Size:       4673
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    198     230    3812    3844     198 
   Size:         32    3582      32    1027

  Fragments arranged by size: 

                3582    1027      32      32
 
PsiI TTA'TAA

Cuts at:    698     818     698 
   Size:        120    4553
 
PspXI vC'TCGA_Gb

Cuts at:   1286    1286 
   Size:       4673
 
PstI C_TGCA'G

Cuts at:    350    1384    1720    2405     350 
   Size:       1034     336     685    2618

  Fragments arranged by size: 

                2618    1034     685     336
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4673
 
PvuII CAG'CTG

Cuts at:   2098    2458    2098 
   Size:        360    4313
 
RsrII CG'GwC_CG

Cuts at:   2868    2868 
   Size:       4673
 
SacI G_AGCT'C

Cuts at:    983    1293    1687     983 
   Size:        310     394    3969

  Fragments arranged by size: 

                3969     394     310
 
SalI G'TCGA_C

Cuts at:   1710    1710 
   Size:       4673
 
SapI GCTCTTCn'nnn_

Cuts at:    158     199     701    2696    2906     158 
   Size:         41     502    1995     210    1925

  Fragments arranged by size: 

                1995    1925     502     210      41
 
SbfI CC_TGCA'GG

Cuts at:   1720    1720 
   Size:       4673
 
ScaI AGT'ACT

Cuts at:   4190    4190 
   Size:       4673
 
SfcI C'TryA_G

Cuts at:     33     346    1380    1500    1716    2401    3394    3585      33 
   Size:        313    1034     120     216     685     993     191    1121

  Fragments arranged by size: 

                1121    1034     993     685     313     216     191     120
            
 
SfoI GGC'GCC

Cuts at:   2105    2353    2105 
   Size:        248    4425
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4673
 
SgrAI Cr'CCGG_yG

Cuts at:    618     618 
   Size:       4673
 
SmaI CCC'GGG

Cuts at:   1695    1695 
   Size:       4673
 
SmlI C'TyrA_G

Cuts at:    457    1286    1372    1828    2743    3235    3497     457 
   Size:        829      86     456     915     492     262    1633

  Fragments arranged by size: 

                1633     915     829     492     456     262      86
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4673
 
SphI G_CATG'C

Cuts at:   1726    2226    1726 
   Size:        500    4173
 
StuI AGG'CCT

Cuts at:    929     929 
   Size:       4673
 
StyI C'CwwG_G

Cuts at:    650     909    1807    2161     650 
   Size:        259     898     354    3162

  Fragments arranged by size: 

                3162     898     354     259
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    654    2256    2389    2848    3057     654 
   Size:       1602     133     459     209    2270

  Fragments arranged by size: 

                2270    1602     459     209     133
 
TatI w'GTAC_w

Cuts at:   1420    4188    1420 
   Size:       2768    1905
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    408     440    1545    1577    4190    4222     408 
   Size:         32    1105      32    2613      32     859

  Fragments arranged by size: 

                2613    1105     859      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2470    2470 
   Size:       4673
 
XbaI T'CTAG_A

Cuts at:   1704    1704 
   Size:       4673
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    648    1127    2168     648 
   Size:        479    1041    3153

  Fragments arranged by size: 

                3153    1041     479
 
XhoI C'TCGA_G

Cuts at:   1286    1286 
   Size:       4673
 
XmaI C'CCGG_G

Cuts at:   1693    1693 
   Size:       4673
 
XmnI GAAnn'nnTTC

Cuts at:   1367    1367 
   Size:       4673
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AleI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI    BstYI
     BtgI    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI
     PmeI     PpiI     PsiI    PspXI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
    SgrAI     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI     AhdI     AlfI     AloI     ApaI     AscI    AvrII
     BaeI    BbvCI    BfrBI     BglI    BmgBI     BmtI    BsiWI     BsmI
   BssHII   BstEII  BstZ17I   Bsu36I    CspCI   DraIII    EcoNI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PpuMI
    PshAI   PspOMI     PsrI    SacII    SanDI    SexAI     SfiI    SnaBI
     SrfI     SspI     SwaI     ZraI
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