################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AviII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BsaAI BsaHI BsaI BseAI BseRI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CfoI ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeII MluI MluNI MroI MscI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AciI 1455 GCGG CCGC(-3/-1) AcsI 1066 AAATTT [GA]^AATT[CT] AflIII 1196 ACATGT A^C[GA][CT]GT AgeI 1364 ACCGGT A^CCGGT AlwI 565 GGATC GGATC(4/5) ApoI 1066 AAATTT [GA]^AATT[CT] Asp700I 690 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 552 GAGCTC G[AT]GC[AT]^C AvaI 1109 CTCGAG C^[CT]CG[GA]G AvrII 213 CCTAGG C^CTAGG BbsI 118 GAAGAC GAAGAC(2/6) BglII 993 AGATCT A^GATCT BlnI 213 CCTAGG C^CTAGG BlpI 601 GCTAAGC GC^T[ACGT]AGC Bpu1102I 601 GCTAAGC GC^T[ACGT]AGC BpuAI 118 GAAGAC GAAGAC(2/6) BsaBI 1238 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 552 GAGCTC G[AT]GC[AT]^C BsoBI 1109 CTCGAG C^[CT]CG[GA]G BspLU11I 1196 ACATGT A^CATGT BsrDI 783 CATTGC GCAATG(2/0) BsrFI 1364 ACCGGT [GA]^CCGG[CT] BstXI 1360 CCAAACCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 993 AGATCT [GA]^GATC[CT] Cac8I 715 GCCAGC GC[ACGT]^[ACGT]GC CelII 601 GCTAAGC GC^T[ACGT]AGC Cfr10I 1364 ACCGGT [GA]^CCGG[CT] DraII 808 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoNI 1218 CCTTTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 808 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoRV 793 GATATC GAT^ATC EcoT14I 213 CCTAGG C^C[AT][AT]GG FauI 1455 GCGGG CCCGC(4/6) HgaI 889 GACGC GACGC(5/10) MamI 1238 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 993 AGATCT [GA]^GATC[CT] MspA1I 717 CAGCTG C[AC]G^C[GT]G MwoI 1118 GCACCAAACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NspI 1196 ACATGT [GA]CATG^[CT] PaeR7I 1109 CTCGAG C^TCGAG PinAI 1364 ACCGGT A^CCGGT PpuMI 808 GGGTCCT [GA]G^G[AT]CC[CT] PstI 788 CTGCAG CTGCA^G PvuII 717 CAGCTG CAG^CTG SacI 552 GAGCTC GAGCT^C ScaI 961 AGTACT AGT^ACT SspI 133 AATATT AAT^ATT StuI 570 AGGCCT AGG^CCT StyI 213 CCTAGG C^C[AT][AT]GG XhoI 1109 CTCGAG C^TCGAG XhoII 993 AGATCT [GA]^GATC[CT] XmnI 690 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AspI 121 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 804 GACAGGGTC BanI 34 GGCACC G^G[CT][GA]CC 1117 GGCACC BanII 552 GAGCTC G[GA]GC[CT]^C 1421 GGGCTC BpmI 1136 CTCCAG CTGGAG(16/14) 515 CTGGAG BsaWI 443 TCCGGT [AT]^CCGG[AT] 1364 ACCGGT BsgI 285 CTGCAC GTGCAG(16/14) 14 GTGCAG BsmI 280 GAATGC GAATGC(1/-1) 1414 GAATGC EarI 918 CTCTTC CTCTTC(1/4) 172 GAAGAG HapII 444 CCGG C^CGG 1365 CCGG HincII 127 GTCAAC GT[CT]^[GA]AC 1090 GTTGAC HindII 127 GTCAAC GT[CT]^[GA]AC 1090 GTTGAC HpaII 444 CCGG C^CGG 1365 CCGG Ksp632I 918 CTCTTC CTCTTC(1/4) 172 GAAGAG MfeI 275 CAATTG C^AATTG 980 CAATTG MslI 844 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1011 CATTCTGGTG MspI 444 CCGG C^CGG 1365 CCGG MunI 275 CAATTG C^AATTG 980 CAATTG PflFI 121 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 804 GACAGGGTC SfaNI 1334 GCATC GCATC(5/9) 1435 GCATC SmlI 965 CTCAAG C^T[CT][GA]AG 1109 CTCGAG TfiI 520 GAATC G^A[AT]TC 609 GATTC Tth111I 121 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 804 GACAGGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AfaI AluI AvaII BbvI BfaI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MvaI NdeII NlaIII NlaIV PleI RcaI RsaI Sau3AI Sau96I ScrFI SfcI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I