Restriction map for pF1KB5892
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp718I     AspI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BcgI        BclI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaI        BsgI        BsiEI       
BsiWI       Bsp1407I    BspDI       BspHI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     CelII       Cfr10I      Cfr13I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        EarI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HpaI        HphI        
KasI        KpnI        Ksp632I     KspI        MfeI        
MluI        MluNI       MscI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        StyI        
SwaI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI           73  GTCTAC               GT^[AC][GT]AC
AccII         145  CGCG                 CG^CG
AccIII        774  TCCGGA               T^CCGGA
AcyI          130  GACGCC               G[GA]^CG[CT]C
AfaI         1024  GTAC                 GT^AC
AflIII        391  ACATGT               A^C[GA][CT]GT
AhdI         1165  GACAACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1298  GTGCAC               G^TGCAC
AocI          637  CCTCAGG              CC^T[ACGT]AGG
ApaLI        1298  GTGCAC               G^TGCAC
AspEI        1165  GACAACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1298  GTGCAC               G[AT]GC[AT]^C
BanII         581  GAGCCC               G[GA]GC[CT]^C
BbvCI        1249  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1046  GGATAC               GTATCC(6/5)
BcnI          104  CCCGG                CC^[GC]GG
BsaAI        1025  TACGTG               [CT]AC^GT[GA]
BsaBI         613  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         130  GACGCC               G[GA]^CG[CT]C
BsaWI         774  TCCGGA               [AT]^CCGG[AT]
BseAI         774  TCCGGA               T^CCGGA
BsiHKAI      1298  GTGCAC               G[AT]GC[AT]^C
BsmBI        1411  GAGACG               CGTCTC(1/5)
BspEI         774  TCCGGA               T^CCGGA
BspLU11I      391  ACATGT               A^CATGT
BstUI         145  CGCG                 CG^CG
Bsu36I        637  CCTCAGG              CC^T[ACGT]AGG
CfoI           57  GCGC                 GCG^C
Eam1105I     1165  GACAACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        637  CCTCAGG              CC^T[ACGT]AGG
FauI          995  GCGGG                CCCGC(4/6)
HaeII          56  AGCGCC               [GA]GCGC^[CT]
HaeIII        721  GGCC                 GG^CC
HgaI          130  GACGC                GACGC(5/10)
HhaI           57  GCGC                 GCG^C
Hin1I         130  GACGCC               G[GA]^CG[CT]C
HinP1I         57  GCGC                 G^CGC
HincII       1354  GTCAAC               GT[CT]^[GA]AC
HindII       1354  GTCAAC               GT[CT]^[GA]AC
MamI          613  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          774  TCCGGA               T^CCGGA
MslI         1563  CACTTCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          145  CGCG                 CG^CG
NciI          104  CCCGG                CC^[GC]GG
NspI          391  ACATGT               [GA]CATG^[CT]
RsaI         1024  GTAC                 GT^AC
SspI          436  AATATT               AAT^ATT


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwI         1371  GATCC                GGATC(4/5)
              819  GGATC               
AlwNI         898  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1177  CAGCCCCTG           
AvaI           82  CCCGAG               C^[CT]CG[GA]G
              154  CCCGAG              
BbsI          521  GAAGAC               GAAGAC(2/6)
             1402  GAAGAC              
BglII          89  AGATCT               A^GATCT
              600  AGATCT              
BmyI          581  GAGCCC               G[AGT]GC[ACT]^C
             1298  GTGCAC              
BpuAI         521  GAAGAC               GAAGAC(2/6)
             1402  GAAGAC              
BseRI          85  GAGGAG               GAGGAG(10/8)
              115  GAGGAG              
BsmFI          79  GTCCC                GGGAC(10/14)
             1173  GTCCC               
BsmI          951  GAATGC               GAATGC(1/-1)
             1500  GAATGC              
BsoBI          82  CCCGAG               C^[CT]CG[GA]G
              154  CCCGAG              
Bsp1286I      581  GAGCCC               G[AGT]GC[ACT]^C
             1298  GTGCAC              
BstYI          89  AGATCT               [GA]^GATC[CT]
              600  AGATCT              
HapII         105  CCGG                 C^CGG
              775  CCGG                
HindIII       187  AAGCTT               A^AGCTT
              366  AAGCTT              
HpaII         105  CCGG                 C^CGG
              775  CCGG                
MaeII          77  ACGT                 A^CGT
             1026  ACGT                
MflI           89  AGATCT               [GA]^GATC[CT]
              600  AGATCT              
MspI          105  CCGG                 C^CGG
              775  CCGG                
TaqI           50  TCGA                 T^CGA
              270  TCGA                
TthHB8I        50  TCGA                 T^CGA
              270  TCGA                
XhoII          89  AGATCT               [GA]^GATC[CT]
              600  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        Asp700I     
BbvI        BfaI        BpmI        BsaJI       BsiYI       
BslI        BsmAI       BspMI       BsrI        BssKI       
BstF5I      BstNI       Cac8I       DdeI        DpnII       
EcoRII      Fnu4HI      FokI        HinfI       ItaI        
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MspA1I      MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        PstI        PvuII       
Sau3AI      ScrFI       SfaNI       SfcI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
XmnI        
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