Restriction map for
pF1KB5855
(Circular) (Six-base) MAPSORT of: pF1KB5855.seq Check: 2842 from: 1 to: 4490
pF1KB5855 4490 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 1528 1528
Size: 4490
Acc65I G'GTAC_C
Cuts at: 1201 1506 1201
Size: 305 4185
AclI AA'CG_TT
Cuts at: 4330 4330
Size: 4490
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 295 646 886 2315 2747 3494 3762 182
Size: 113 351 240 1429 432 747 268 910
Fragments arranged by size:
1429 910 747 432 351 268 240 113
AfeI AGC'GCT
Cuts at: 1372 1372
Size: 4490
AflIII A'CryG_T
Cuts at: 1842 2946 3923 1842
Size: 1104 977 2409
Fragments arranged by size:
2409 1104 977
AgeI A'CCGG_T
Cuts at: 2878 2878
Size: 4490
AleI CACnn'nnGTG
Cuts at: 304 1052 304
Size: 748 3742
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 460 929 460
Size: 469 4021
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1377 1409 1377
Size: 32 4458
AlwNI CAG_nnn'CTG
Cuts at: 408 754 1221 1795 3362 408
Size: 346 467 574 1567 1536
Fragments arranged by size:
1567 1536 574 467 346
ApaI G_GGCC'C
Cuts at: 1241 1334 1241
Size: 93 4397
ApaLI G'TGCA_C
Cuts at: 3260 3260
Size: 4490
ApoI r'AATT_y
Cuts at: 476 1494 3843 4099 476
Size: 1018 2349 256 867
Fragments arranged by size:
2349 1018 867 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4490
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4490
AvaI C'yCGr_G
Cuts at: 365 1349 1415 1510 365
Size: 984 66 95 3345
Fragments arranged by size:
3345 984 95 66
BamHI G'GATC_C
Cuts at: 955 1515 955
Size: 560 3930
BanI G'GyrC_C
Cuts at: 339 1166 1201 1506 1920 2168 2203 339
Size: 827 35 305 414 248 35 2626
Fragments arranged by size:
2626 827 414 305 248 35 35
BanII G_rGCy'C
Cuts at: 719 1241 1253 1334 1504 2534 719
Size: 522 12 81 170 1030 2675
Fragments arranged by size:
2675 1030 522 170 81 12
BbeI G_GCGC'C
Cuts at: 1924 2172 1924
Size: 248 4242
BciVI GTATCCnnnnn_n'
Cuts at: 338 2388 3149 4490 338
Size: 2050 761 1341 338
Fragments arranged by size:
2050 1341 761 338
BclI T'GATC_A
Cuts at: 2010 2010
Size: 4490
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4490
BglII A'GATC_T
Cuts at: 239 347 2005 4042 239
Size: 108 1658 2037 687
Fragments arranged by size:
2037 1658 687 108
BlpI GC'TnA_GC
Cuts at: 875 1602 875
Size: 727 3763
Bme1580I G_kGCm'C
Cuts at: 439 1241 1334 2115 2208 3264 439
Size: 802 93 781 93 1056 1665
Fragments arranged by size:
1665 1056 802 781 93 93
BmrI ACTGGGnnnn_n'
Cuts at: 156 782 1127 1702 1783 2118 4262 156
Size: 626 345 575 81 335 2144 384
Fragments arranged by size:
2144 626 575 384 345 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 736 2857 736
Size: 2121 2369
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 889 1228 1666 2545 3037 3335 889
Size: 339 438 879 492 298 2044
Fragments arranged by size:
2044 879 492 438 339 298
BsaI GGTCTCn'nnnn_
Cuts at: 4194 4194
Size: 4490
BsaAI yAC'GTr
Cuts at: 417 1843 417
Size: 1426 3064
BsaBI GATnn'nnATC
Cuts at: 6 244 352 2004 4041 6
Size: 238 108 1652 2037 455
Fragments arranged by size:
2037 1652 455 238 108
BsaHI Gr'CG_yC
Cuts at: 115 1921 2169 2871 4367 115
Size: 1806 248 702 1496 238
Fragments arranged by size:
1806 1496 702 248 238
BsaWI w'CCGG_w
Cuts at: 1679 2200 2878 3152 3299 3710 1679
Size: 521 678 274 147 411 2459
Fragments arranged by size:
2459 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 1016 154
Size: 862 3628
BseYI C'CCAG_C
Cuts at: 318 408 427 432 967 1043 1364 1915 2673
Size: 90 19 5 535 76 321 551 758
Cuts at: 2673 3250 3746 318
Size: 577 496 1062
Fragments arranged by size:
1062 758 577 551 535 496 321 90
76 19 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 729 812 715
Size: 14 83 4393
Fragments arranged by size:
4393 83 14
BsiEI CG_ry'CG
Cuts at: 104 2032 2078 3286 104
Size: 1928 46 1208 1308
Fragments arranged by size:
1928 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1504 2282 2472 3264 1504
Size: 778 190 792 2730
Fragments arranged by size:
2730 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 439 719 1241 1253 1334 1504 2115 2208 2282
Size: 280 522 12 81 170 611 93 74
Cuts at: 2282 2472 2534 3264 439
Size: 190 62 730 1665
Fragments arranged by size:
1665 730 611 522 280 190 170 93
81 74 62 12
BspEI T'CCGG_A
Cuts at: 3710 3710
Size: 4490
BspHI T'CATG_A
Cuts at: 3666 3666
Size: 4490
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1540 1754 2056 2437 1540
Size: 214 302 381 3593
Fragments arranged by size:
3593 381 302 214
BsrBI CCG'CTC
Cuts at: 50 849 2782 2836 4320 4485 50
Size: 799 1933 54 1484 165 55
Fragments arranged by size:
1933 1484 799 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2402 2402
Size: 4490
BsrFI r'CCGG_y
Cuts at: 1433 2488 2669 2878 1433
Size: 1055 181 209 3045
Fragments arranged by size:
3045 1055 209 181
BssSI C'ACGA_G
Cuts at: 721 2761 3119 721
Size: 2040 358 2092
Fragments arranged by size:
2092 2040 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1867 1867
Size: 4490
BstBI TT'CG_AA
Cuts at: 2851 2851
Size: 4490
BstYI r'GATC_y
Cuts at: 1 239 347 386 955 1119 1515 1982 2005
Size: 238 108 39 569 164 396 467 23
Cuts at: 2005 2340 2586 3598 3684 3696 4042 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 569 467 449 396 346 335 246
238 164 108 86 39 23 12
Bsu36I CC'TnA_GG
Cuts at: 1232 1232
Size: 4490
BtgI C'CryG_G
Cuts at: 107 232 770 1253 107
Size: 125 538 483 3344
Fragments arranged by size:
3344 538 483 125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2440 2621 2784 2440
Size: 181 163 4146
Fragments arranged by size:
4146 181 163
BtsI GCAGTG_nn'
Cuts at: 263 437 579 585 703 1733 263
Size: 174 142 6 118 1030 3020
Fragments arranged by size:
3020 1030 174 142 118 6
DraI TTT'AAA
Cuts at: 1489 1489
Size: 4490
DrdI GACnn_nn'nnGTC
Cuts at: 2196 3054 2196
Size: 858 3632
EaeI y'GGCC_r
Cuts at: 419 504 2075 2249 2640 2667 4012 419
Size: 85 1571 174 391 27 1345 897
Fragments arranged by size:
1571 1345 897 391 174 85 27
EagI C'GGCC_G
Cuts at: 2075 2075
Size: 4490
EarI CTCTTCn'nnn_
Cuts at: 2513 2723 2513
Size: 210 4280
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2698 3008 3154 4300 2698
Size: 310 146 1146 2888
Fragments arranged by size:
2888 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1502 1502
Size: 4490
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 182 295 646 886 2315 2747 3494 3762 182
Size: 113 351 240 1429 432 747 268 910
Fragments arranged by size:
1429 910 747 432 351 268 240 113
EcoO109I rG'GnC_Cy
Cuts at: 150 315 1141 1237 1238 1294 1312 1330 1331
Size: 165 826 96 1 56 18 18 1
Cuts at: 1331 1629 150
Size: 298 3011
Fragments arranged by size:
3011 826 298 165 96 56 18 18
1 1
EcoRI G'AATT_C
Cuts at: 1494 1494
Size: 4490
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 564 2509 564
Size: 1945 2545
FspI TGC'GCA
Cuts at: 2271 2271
Size: 4490
HaeII r_GCGC'y
Cuts at: 1374 1924 2172 3194 4173 1374
Size: 550 248 1022 979 1691
Fragments arranged by size:
1691 1022 979 550 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 721 753 878 910 2332 2362 2364 2394 721
Size: 32 125 32 1422 30 2 30 2817
Fragments arranged by size:
2817 1422 125 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1529 1529
Size: 4490
KasI G'GCGC_C
Cuts at: 1920 2168 1920
Size: 248 4242
KpnI G_GTAC'C
Cuts at: 1205 1510 1205
Size: 305 4185
MluI A'CGCG_T
Cuts at: 3923 3923
Size: 4490
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3161 3345 3161
Size: 184 4306
MscI TGG'CCA
Cuts at: 421 506 2251 421
Size: 85 1745 2660
Fragments arranged by size:
2660 1745 85
MslI CAynn'nnrTG
Cuts at: 304 442 1052 2479 2606 304
Size: 138 610 1427 127 2188
Fragments arranged by size:
2188 1427 610 138 127
MspA1I CmG'CkG
Cuts at: 130 937 1322 1601 1915 2275 3288 3533 130
Size: 807 385 279 314 360 1013 245 1087
Fragments arranged by size:
1087 1013 807 385 360 314 279 245
NaeI GCC'GGC
Cuts at: 2671 2671
Size: 4490
NarI GG'CG_CC
Cuts at: 1921 2169 1921
Size: 248 4242
NcoI C'CATG_G
Cuts at: 107 232 770 1253 107
Size: 125 538 483 3344
Fragments arranged by size:
3344 538 483 125
NdeI CA'TA_TG
Cuts at: 1051 1051
Size: 4490
NgoMIV G'CCGG_C
Cuts at: 2669 2669
Size: 4490
NspI r_CATG'y
Cuts at: 667 1543 2043 2950 667
Size: 876 500 907 2207
Fragments arranged by size:
2207 907 876 500
PasI CC'CwG_GG
Cuts at: 1458 1458
Size: 4490
PciI A'CATG_T
Cuts at: 2946 2946
Size: 4490
PflMI CCAn_nnn'nTGG
Cuts at: 612 612
Size: 4490
PfoI T'CCnGG_A
Cuts at: 533 533
Size: 4490
PmeI GTTT'AAAC
Cuts at: 1489 1489
Size: 4490
PmlI CAC'GTG
Cuts at: 417 417
Size: 4490
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3629 3661 3629
Size: 32 4458
PpuMI rG'GwC_Cy
Cuts at: 150 315 1141 1294 150
Size: 165 826 153 3346
Fragments arranged by size:
3346 826 165 153
PspOMI G'GGCC_C
Cuts at: 1237 1330 1237
Size: 93 4397
PstI C_TGCA'G
Cuts at: 129 135 270 1537 2222 129
Size: 6 135 1267 685 2397
Fragments arranged by size:
2397 1267 685 135 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4490
PvuII CAG'CTG
Cuts at: 130 937 1322 1915 2275 130
Size: 807 385 593 360 2345
Fragments arranged by size:
2345 807 593 385 360
RsrII CG'GwC_CG
Cuts at: 2685 2685
Size: 4490
SacI G_AGCT'C
Cuts at: 1504 1504
Size: 4490
SalI G'TCGA_C
Cuts at: 1527 1527
Size: 4490
SapI GCTCTTCn'nnn_
Cuts at: 2513 2723 2513
Size: 210 4280
SbfI CC_TGCA'GG
Cuts at: 1537 1537
Size: 4490
ScaI AGT'ACT
Cuts at: 4007 4007
Size: 4490
SexAI A'CCwGG_T
Cuts at: 1072 1072
Size: 4490
SfcI C'TryA_G
Cuts at: 33 125 131 199 266 938 1533 2218 3211
Size: 92 6 68 67 672 595 685 993
Cuts at: 3211 3402 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 672 595 191 92 68
67 6
SfoI GGC'GCC
Cuts at: 1922 2170 1922
Size: 248 4242
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4490
SmaI CCC'GGG
Cuts at: 1512 1512
Size: 4490
SmlI C'TyrA_G
Cuts at: 868 1243 1645 2560 3052 3314 868
Size: 375 402 915 492 262 2044
Fragments arranged by size:
2044 915 492 402 375 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4490
SphI G_CATG'C
Cuts at: 667 1543 2043 667
Size: 876 500 3114
Fragments arranged by size:
3114 876 500
StuI AGG'CCT
Cuts at: 547 547
Size: 4490
StyI C'CwwG_G
Cuts at: 107 232 422 770 1253 1281 1624 1978 107
Size: 125 190 348 483 28 343 354 2619
Fragments arranged by size:
2619 483 354 348 343 190 125 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 981 1184 1228 2073 2206 2665 2874 981
Size: 203 44 845 133 459 209 2597
Fragments arranged by size:
2597 845 459 209 203 133 44
TatI w'GTAC_w
Cuts at: 4005 4005
Size: 4490
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 240 272 989 1021 4007 4039 240
Size: 32 717 32 2986 32 691
Fragments arranged by size:
2986 717 691 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 148 1074 2287 148
Size: 926 1213 2351
Fragments arranged by size:
2351 1213 926
XbaI T'CTAG_A
Cuts at: 189 1521 189
Size: 1332 3158
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 429 1985 429
Size: 1556 2934
XmaI C'CCGG_G
Cuts at: 1510 1510
Size: 4490
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AscI AvrII BaeI BbsI
BbvCI BcgI BfrBI BmgBI BmtI BpmI Bpu10I BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NheI NotI NruI NsiI PacI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI