Restriction map for pF1KB5824
 (Linear) (Six-base) MAPSORT of: KIBB5824.seq  Check: 1338  from: 1  to: 1428



KIBB5824.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AccI GT'mk_AC

Cuts at:      0     137    1428 
   Size:        137    1291
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     556     821    1387    1428 
   Size:        113     443     265     566      41

  Fragments arranged by size: 

                 566     443     265     113      41
 
AflIII A'CryG_T

Cuts at:      0    1042    1428 
   Size:       1042     386
 
ApoI r'AATT_y

Cuts at:      0     205     547    1284    1428 
   Size:        205     342     737     144

  Fragments arranged by size: 

                 737     342     205     144
 
AvaI C'yCGr_G

Cuts at:      0     827    1428 
   Size:        827     601
 
BamHI G'GATC_C

Cuts at:      0    1090    1428 
   Size:       1090     338
 
BanI G'GyrC_C

Cuts at:      0      52    1081    1428 
   Size:         52    1029     347

  Fragments arranged by size: 

                1029     347      52
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      26    1428 
   Size:         26    1402
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     978    1428 
   Size:        978     450
 
BclI T'GATC_A

Cuts at:      0    1050    1428 
   Size:       1050     378
 
BglII A'GATC_T

Cuts at:      0     164    1428 
   Size:        164    1264
 
Bme1580I G_kGCm'C

Cuts at:      0      11    1428 
   Size:         11    1417
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      33     626     854    1428 
   Size:         33     593     228     574

  Fragments arranged by size: 

                 593     574     228      33
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     509     629     887     927    1428 
   Size:        271     238     120     258      40     501

  Fragments arranged by size: 

                 501     271     258     238     120      40
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     790    1428 
   Size:        790     638
 
BsaBI GATnn'nnATC

Cuts at:      0     169    1428 
   Size:        169    1259
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     281     701    1428 
   Size:        281     420     727

  Fragments arranged by size: 

                 727     420     281
 
BseYI C'CCAG_C

Cuts at:      0    1034    1428 
   Size:       1034     394
 
Bsp1286I G_dGCh'C

Cuts at:      0      11    1428 
   Size:         11    1417
 
BspHI T'CATG_A

Cuts at:      0     189    1428 
   Size:        189    1239
 
BsrDI GCAATG_nn'

Cuts at:      0     605    1428 
   Size:        605     823
 
BstYI r'GATC_y

Cuts at:      0     164    1090    1428 
   Size:        164     926     338

  Fragments arranged by size: 

                 926     338     164
 
BtgI C'CryG_G

Cuts at:      0     619    1428 
   Size:        619     809
 
BtsI GCAGTG_nn'

Cuts at:      0      11     113    1428 
   Size:         11     102    1315

  Fragments arranged by size: 

                1315     102      11
 
ClaI AT'CG_AT

Cuts at:      0     701    1428 
   Size:        701     727
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     483     518    1428 
   Size:        483      35     910

  Fragments arranged by size: 

                 910     483      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     532     537    1428 
   Size:        532       5     891

  Fragments arranged by size: 

                 891     532       5
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     271     509     556     629     821     887     927 
   Size:        113     158     238      47      73     192      66      40

Cuts at:    927    1387    1428 
   Size:        460      41

  Fragments arranged by size: 

                 460     238     192     158     113      73      66      47
                  41      40
 
EcoO109I rG'GnC_Cy

Cuts at:      0     824    1428 
   Size:        824     604
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      88    1377    1428 
   Size:         88    1289      51

  Fragments arranged by size: 

                1289      88      51
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     559     591     733     765    1428 
   Size:        559      32     142      32     663

  Fragments arranged by size: 

                 663     559     142      32      32
 
HincII GTy'rAC

Cuts at:      0    1317    1428 
   Size:       1317     111
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     392    1428 
   Size:        392    1036
 
MslI CAynn'nnrTG

Cuts at:      0     194     301    1428 
   Size:        194     107    1127

  Fragments arranged by size: 

                1127     194     107
 
MspA1I CmG'CkG

Cuts at:      0     641    1113    1428 
   Size:        641     472     315

  Fragments arranged by size: 

                 641     472     315
 
NcoI C'CATG_G

Cuts at:      0     619    1428 
   Size:        619     809
 
NdeI CA'TA_TG

Cuts at:      0     516    1055    1428 
   Size:        516     539     373

  Fragments arranged by size: 

                 539     516     373
 
NspI r_CATG'y

Cuts at:      0     306    1046    1428 
   Size:        306     740     382

  Fragments arranged by size: 

                 740     382     306
 
PasI CC'CwG_GG

Cuts at:      0      42    1428 
   Size:         42    1386
 
PciI A'CATG_T

Cuts at:      0    1042    1428 
   Size:       1042     386
 
PshAI GACnn'nnGTC

Cuts at:      0     641    1428 
   Size:        641     787
 
PvuII CAG'CTG

Cuts at:      0     641    1428 
   Size:        641     787
 
ScaI AGT'ACT

Cuts at:      0     256     590     940    1428 
   Size:        256     334     350     488

  Fragments arranged by size: 

                 488     350     334     256
 
SfcI C'TryA_G

Cuts at:      0     258    1428 
   Size:        258    1170
 
SmlI C'TyrA_G

Cuts at:      0     805    1428 
   Size:        805     623
 
SphI G_CATG'C

Cuts at:      0     306    1428 
   Size:        306    1122
 
SspI AAT'ATT

Cuts at:      0     153     241    1310    1428 
   Size:        153      88    1069     118

  Fragments arranged by size: 

                1069     153     118      88
 
StyI C'CwwG_G

Cuts at:      0     619     688    1428 
   Size:        619      69     740

  Fragments arranged by size: 

                 740     619      69
 
TatI w'GTAC_w

Cuts at:      0     254     588     938    1428 
   Size:        254     334     350     490

  Fragments arranged by size: 

                 490     350     334     254
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     868     900    1428 
   Size:        868      32     528

  Fragments arranged by size: 

                 868     528      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     569    1428 
   Size:        569     859
 
XmnI GAAnn'nnTTC

Cuts at:      0     404    1428 
   Size:        404    1024
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     ApoI     AvaI    BamHI     BanI     BbsI
    BciVI     BclI    BglII Bme1580I     BmrI     BpmI    BpuEI    BsaBI
    BseRI    BseYI Bsp1286I    BspHI    BsrDI    BstYI     BtgI     BtsI
     ClaI    CspCI     EarI  Eco57MI EcoO109I     FalI    Hin4I   HincII
     MmeI     MslI   MspA1I     NcoI     NdeI     NspI     PasI     PciI
    PshAI    PvuII     ScaI     SfcI     SmlI     SphI     SspI     StyI
     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BanII     BbeI    BbvCI     BcgI    BfrBI
     BglI     BlpI    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     StuI     SwaI    TaqII  Tth111I     XbaI     XhoI     XmaI     ZraI
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